MEASLES VIRUSES WITH ADDED VACCINE SPECIFICITIES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本发明的麻疹减毒活疫苗具有卓越的效果和安全记录，并能诱导持久免疫。我们假设，目前使用的麻疹病毒(MV)疫苗株是开发儿童疫苗的理想平台，该疫苗可激发对其他病原体的免疫。我们将检验这一假设的两个命题：第一，载体MVS可以预防另一种病原体的感染。其次，它们可以引起对其他人类病原体的强大免疫反应，同时保持针对麻疹的疫苗功能。表达不同水平的乙肝病毒表面抗原的重组MV将被用来确定最有效地诱导强烈体液免疫反应的表达策略。那些在转基因小鼠中诱导对乙肝免疫反应最强的病毒将被接种到幼猴体内。将测量它们在保护这些灵长类动物免受野生型麻疹感染和诱导对额外病原体的免疫反应方面的有效性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The live attenuated measles vaccine has an outstanding efficiency and safety record and induces enduring immunity. We hypothesize that a currently used measles virus (MV) vaccine strain is an ideal platform for the development of pediatric vaccines eliciting immunity against other pathogens. We will test two propositions of this hypothesis: first that vectored MVs can protect against infection with another pathogen. Second, that they can elicit a strong immune response against other human pathogens whilst maintaining vaccine function against measles. Recombinant MV expressing the surface antigen of the hepatitis B virus at different levels will be used to define the expression strategy most effective in inducing a strong humoral immune response. Those viruses inducing the strongest immune response against hepatitis B in genetically modified mice will be inoculated into juvenile macaques. Their efficacy in protecting these primates against wild type measles infection and in inducing an immune response against the additional pathogen will be measured.