NOVEL TECHNIQUES FOR ANALYSIS OF BONE MINERAL BALANCE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：探讨钙、锶稳定同位素技术在骨矿平衡评价中的应用价值。 骨质疏松症是老年人最常见的疾病之一，其特征是低骨量和骨小梁结构紊乱，伴有骨骼脆弱。在世界范围内，骨质疏松症影响50岁以上的三分之一女性和四分之一男性。此外，随着人口规模和预期寿命的增加，预计在未来20年内，骨质疏松相关髋部骨折的发病率将翻一番。营养不良、遗传易感性、体力活动减少、“年龄相关”因素和雌激素缺乏都可能导致绝经后和年龄相关性骨质疏松症的病理生理学。绝经或卵巢切除导致循环雌激素水平降低后，骨转换和骨丢失迅速增加，突出了雌激素在女性骨质疏松症发生中的关键作用。骨转换的生化标志物具有边际临床价值，不适合检测骨矿物质动力学（骨骼对内分泌变化的反应的关键组成部分）的快速变化，并且不能用于测量净骨矿物质平衡。为了克服这些问题，我们建议采用钙（Ca）和锶（Sr）稳定同位素技术，在地球化学中得到很好的验证，但很少应用于生物医学。这些技术，这承诺提供前所未有的详细和精确的骨矿物质平衡的数据，将被验证和应用于一个独特的动物模型的低雌激素骨量维持。这些新的，非侵入性的技术将被证明是非常宝贵的骨质疏松症的研究，并为未来的研究和临床应用提供了很高的承诺。本研究使用动物服务和研究服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To explore the possible utility of calcium and strontium stable isotope techniques in the assessment of bone mineral balance. Osteoporosis, characterized by low bone mass and disordered trabecular architecture with attendant skeletal fragility, is among the most common diseases of older people. Worldwide, osteoporosis affects 1 in 3 women and 1 in 4 men over 50 years of age. Additionally, the incidence of osteoporosis-related hip fracture is expected to double in the next 20yrs with increasing population size and life expectancy. Nutritional insufficiencies, genetic predisposition, decreased physical activity, "age-related" factors, and estrogen deficiency all likely contribute to the pathophysiology of postmenopausal and age-related osteoporosis. Skeletal turnover and bone loss rapidly increase following reduction of circulating estrogen levels resulting from menopause or ovariectomy, highlighting the critical role of estrogen in the genesis of osteoporosis in women. Biochemical markers of bone turnover are of marginal clinical value, are poorly suited to detect rapid alterations in bone mineral kinetics (critical components of skeletal response to endocrine change), and cannot be used to measure net bone mineral balance. To overcome these problems, we propose employing calcium (Ca) and strontium (Sr) stable isotope techniques that are well validated in geochemistry, but rarely applied in biomedicine. These techniques, which promise to provide data on bone mineral balance of unprecedented detail and precision, will be validated and applied to a unique animal model of hypoestrogenic bone mass maintenance. These novel, noninvasive techniques will prove invaluable to osteoporosis research and afford high promise for future research and clinical applications. This research used Animal Services and Research Services.