Translation analysis of a novel intervention for diet-induced obesity

项目摘要 年龄越大，患2型糖尿病的风险越高，而年龄越大，患2型糖尿病的风险越高。 老年人肥胖症患病率。这些疾病是与年龄相关的疾病的危险因素 严重的发病率和死亡率，包括癌症、心血管疾病和阿尔茨海默病。新的 因此，迫切需要在老年人中维持血糖稳态的方法。该计划的目标是 拟议的研究是测试一种减少支链氨基酸的饮食，最近在小鼠身上显示了逆转饮食 诱导肥胖和胰岛素抵抗，在一种小型非人灵长类动物中，常见的绒猴。我们也将从中获益 从分子水平探讨膳食支链氨基酸减少的机制 绒猴血液和骨骼肌样品的比较代谢组学和蛋白质组学分析 来自幼年和老年小鼠。这些研究迫切需要了解是否操纵特定的饮食 氨基酸是一种可翻译的干预措施，可促进新陈代谢健康，延长健康寿命。

Project Summary Advancing age is associated with an increased risk of type 2 diabetes, which is exacerbated by the high prevalence of obesity in the aged. These disorders are risk factors for age-related diseases associated with significant morbidity and mortality, including cancer, cardiovascular disease, and Alzheimer’s disease. New approaches to maintain glucose homeostasis in the aged are therefore urgently needed. The objective of the proposed study is to test a reduced branched-chain amino acid diet, recently shown in mice to reverse diet induced obesity and insulin resistance, in a small nonhuman primate, the common marmoset. We will also gain insight into the molecular mechanisms induced by reduced dietary branched-chain amino acids through comparative metabolomic and proteomic analysis of blood and skeletal muscle samples from marmosets and from young and aged mice. These studies are urgently needed to understand if manipulation of specific dietary amino acids is a translatable intervention to promote metabolic health and increase healthspan.