GENES CONTROLLING PUBERTY ONSET

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：研究控制青春期发育的基因。 了解青春期在大脑中的机制很重要，因为一些疾病和行为障碍与青春期有关。在这项研究中，我们研究了Kispeptin在青春期LHRH释放增加中的作用。结果表明，用微透析法测量的Kispeptin-54在茎正中隆起的释放是脉动性的，大约75%的Kispeptin-54脉冲与LHRH脉冲相关。此外，Kispeptin-10以剂量依赖的方式刺激LHRH的释放。最后，在通过推拉灌注法收集的“存档样本”中测量了Kispeptin-54，在这种方法中，LHRH水平以前已经被测量过。结果表明，Kispeptin-54的释放与青春期LHRH-1的释放增加有关，并且在青春期前的猴子中已经观察到Kispeptin-54的释放在夜间增加，并持续到青春期。LHRH-1的释放在夜间增加，出现在青春期早期和青春期中期，但在青春期前的猴子中没有。总体而言，这些结果与Kispeptin在青春期起作用的假设是一致的。这项研究使用了WNPRC动物服务和化验服务。 了解青春期在大脑中的机制很重要，因为一些疾病和行为障碍与青春期有关。在这项研究中，我们研究了Kispeptin在青春期LHRH释放增加中的作用。结果表明，用微透析法测量的Kispeptin-54在茎正中隆起的释放是脉动性的，大约75%的Kispeptin-54脉冲与LHRH脉冲相关。此外，在通过推拉灌注法收集的“存档样本”中检测到了Kispeptin-54，在这种方法中，LHRH水平已经被测量过。结果表明，Kispeptin-54的释放与青春期LHRH-1的释放增加有关，并且在青春期前的猴子中已经观察到Kispeptin-54的释放在夜间增加，并持续到青春期。LHRH-1的释放在夜间增加，出现在青春期早期和青春期中期，但在青春期前的猴子中没有。最后，Kispeptin-10以剂量依赖的方式刺激青春期前和青春期猴子的LHRH释放。总体而言，这些结果与Kispeptin在青春期起作用的假设是一致的。这项研究使用了WNPRC动物服务和化验服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To investigate genes controlling the timing of puberty. Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. In this study the role of kisspeptin in the pubertal increase in LHRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile, and approximately 75% of kisspeptin-54 pulses were correlated with LHRH pulses. Moreover, kisspeptin-10 stimulated LHRH release in a dose-dependent manner. Finally, kisspeptin-54 was measured in "archived samples" collected by a push-pull perfusion method, in which LHRH levels had been previously measured. The results indicate that a significant increase in kisspeptin-54 release occurred in association with the pubertal increase in LHRH-1 release and that a nocturnal increase in kisspeptin-54 release was already observed in prepubertal monkeys and continued through the pubertal period. A nocturnal increase in LHRH-1 release occurred in early pubertal and midpubertal, but not in prepubertal monkeys. Collectively, the results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services. Understanding the mechanism of puberty in the brain is important, as some diseases and behavioral disturbances occur in association with puberty. In this study the role of kisspeptin in the pubertal increase in LHRH release was examined. The results suggest that kisspeptin-54 release in the stalk-median eminence measured by a microdialysis method was pulsatile, and approximately 75% of kisspeptin-54 pulses were correlated with LHRH pulses. Moreover, kisspeptin-54 was measured in "archived samples" collected by a push-pull perfusion method, in which LHRH levels had been previously measured. The results indicate that a significant increase in kisspeptin-54 release occurred in association with the pubertal increase in LHRH-1 release and that a nocturnal increase in kisspeptin-54 release was already observed in prepubertal monkeys and continued through the pubertal period. A nocturnal increase in LHRH-1 release occurred in early pubertal and midpubertal, but not in prepubertal monkeys. Finally, kisspeptin-10 stimulated LHRH release in a dose-dependent manner in both prepubertal and pubertal monkeys. Collectively, the results are consistent with the hypothesis that kisspeptin plays a role in puberty. This research used WNPRC Animal Services and Assay Services.