MECHANISM OF EXCESS GLUCOSE PRODUCTION IN TYPE 2 DIABETES

2 型糖尿病中葡萄糖产生过多的机制

• 批准号: 8363906
• 负责人: EUNSOOK JIN
• 金额: $ 1.61万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363906
• 关键词: Animals; Clinical; Data; Development; Diabetes Mellitus; Diagnosis; Diet; Disease; Epidemic; Fasting; Fatty acid glycerol esters; Funding; Gluconeogenesis; Glucose; Glycogenolysis Inhibition; Goals; Grant; Hepatic; Homeostasis; Human; Hydrolysis; Hyperglycemia; In Vitro; Individual; Insulin Resistance; Liver; Liver Failure; Liver Glycogen; Metabolic; Metabolism; Methodology; National Center for Research Resources; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Observational Study; Patients; Physiology; Principal Investigator; Regulation; Reporting; Research; Research Infrastructure; Resources; Rodent Model; Role; Source; United States National Institutes of Health; Work; carbohydrate metabolism; cost; feeding; glucose metabolism; glucose production; glycogen metabolism; glycogenolysis; in vivo; lipid metabolism; prevent; stable isotope

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the work is to undertake observational studies using in vitro and in vivo NMR and stable isotope methodologies to assess glycogen metabolism and its regulation in normal physiology and in obesity-associated diabetes in high-fat-fed and fasted conditions. Type 2 diabetes mellitus (T2DM) is getting epidemic threatening millions of people and its increase is tightly related with the rapid increase of obesity throughout the world. Insulin resistance may proceed decades before the onset of diabetes and it is common in obese individuals. My long term goal in research is finding primary cause(s) of insulin resistance and elucidating the interactions between glucose and lipid metabolism in T2DM, which are essential for strategy development to prevent the onset of diabetes and to fine a cure or better treatments for the disease. A key clinical observation for diagnosis of diabetes is fasting hyperglycemia. Hepatic glucose overproduction contributes fasting hyperglycemia, but controversial data have been reported about the roles of glycogenolysis and gluconeogenesis in T2DM. Recently I found that preserved liver glycogen in fasting resulted in excess glycogenolysis, and subsequently contributed fasting hyperglycemia in rodent models for obesityassociated T2DM. This observation could be important in understanding the failure of hepatic glucose autoregulation in obese T2DM patients because increased fasting hepatic glycogen was reported in obese humans and T2DM patients. Excess liver glycogen in fasting could be critical in fasting hyperglycemia in those individuals because endogenous glucose production is sensitive to the amount of hepatic glycogen available for hydrolysis. In this proposal, liver metabolic changes in obesity and in obesity-associated T2DM will be evaluated in the connection with systemic metabolic fluxes of whole animals. A short-term high-fat diet induced hepatic insulin resistance and the interaction between lipid and carbohydrate metabolism in obesity-associated T2DM will be evaluated focusing hepatic glycogen. It will be determined whether inhibition of glycogenolysis alters fasting hyperglycemia in rodent models of obesity-associated T2DM.

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