Antibody Shared Resources

项目总结（见说明）： 抗体共享资源为CSHL癌症中心人员生产单克隆和多克隆抗体提供了一个中心网站。该共享资源的目的是提供资源和专业知识，以高效和经济的方式生产这些有价值的试剂。 在单克隆抗体方面，CSHL癌症中心的科学家提供特异性抗原，共享资源人员负责免疫、融合、杂交瘤初筛、细胞培养、单细胞克隆、杂交瘤系冷冻和储存的各个阶段，以及细胞培养或体内大规模生产单克隆抗体。与动物共享资源的人员协调免疫接种、采血试验和腹水生产。特异性单克隆抗体 屏幕与科学家个人密切协调。多克隆抗体生产外包，但共享资源人员负责处理和分发样品，从而降低成本并最大限度地提高效率。共享资源还测试和实施新技术，试剂， 以及与抗体产生的不同阶段相关的测定改进。

PROJECT SUMMARY (See instructions): The Antibody Shared Resource provides a central site for the production of monoclonal and polyclonal antibodies by CSHL Cancer Center personnel. The purpose of this Shared Resource is to provide the resources and expertise to generate these valuable reagents in a highly efficient and cost-effective manner. In the ease of monoclonal antibodies, CSHL Cancer Center scientists provide specific antigens, and the Shared Resource personnel handle all stages of immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and storage of hybridoma lines, and large-scale production of monoclonal antibodies in cell culture or in vivo. Immunization, test bleeds, and production of ascites fluids are coordinated with the personnel of the Animal Shared Resource. Specialized monoclonal antibody screens are closely coordinated with individual scientists. Polyclonal antibody production is outsourced, but the Shared Resource personnel are in charge of processing and distribution of samples, thus reducing the costs and maximizing efficiency. The Shared Resource also tests and implements new techniques, reagents, and assay improvements related to the different stages of antibody production.