Antibody Shared Resources

项目总结(见说明)： 抗体共享资源为CSHL癌症中心工作人员生产单抗和多克隆抗体提供了一个中心站点。这一共享资源的目的是提供资源和专业知识，以高效和具有成本效益的方式生产这些有价值的试剂。 在单抗方面，CSHL癌症中心的科学家提供特定的抗原，共享资源人员负责免疫、融合、杂交瘤的初步筛选、细胞培养、单细胞克隆、杂交瘤株的冷冻和储存以及在细胞培养或体内大规模生产单抗的所有阶段。免疫接种、测试出血和腹水产生与动物共享资源的人员进行协调。特异型单抗 屏幕与个别科学家密切协调。多克隆抗体的生产被外包，但共享资源人员负责样本的处理和分发，从而降低了成本，最大限度地提高了效率。共享资源还测试和实施新技术、试剂、 以及与抗体产生的不同阶段相关的检测改进。

PROJECT SUMMARY (See instructions): The Antibody Shared Resource provides a central site for the production of monoclonal and polyclonal antibodies by CSHL Cancer Center personnel. The purpose of this Shared Resource is to provide the resources and expertise to generate these valuable reagents in a highly efficient and cost-effective manner. In the ease of monoclonal antibodies, CSHL Cancer Center scientists provide specific antigens, and the Shared Resource personnel handle all stages of immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and storage of hybridoma lines, and large-scale production of monoclonal antibodies in cell culture or in vivo. Immunization, test bleeds, and production of ascites fluids are coordinated with the personnel of the Animal Shared Resource. Specialized monoclonal antibody screens are closely coordinated with individual scientists. Polyclonal antibody production is outsourced, but the Shared Resource personnel are in charge of processing and distribution of samples, thus reducing the costs and maximizing efficiency. The Shared Resource also tests and implements new techniques, reagents, and assay improvements related to the different stages of antibody production.