Microscopy

显微镜检查

• 批准号: 8340294
• 负责人: BRUCE W. STILLMAN
• 金额: $ 29.06万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-17 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340294
• 关键词: Antibodies; Cancer Center; Cancer Center Support Grant; Carbon; Cell Fractionation; Cells; Confocal Microscopy; Coupled; Cultured Cells; DNA; Dissection; Electron Microscope; Electron Microscopy; Equipment; Evaluation; Fluorescence Microscopy; Fluorochrome; Glass; Gold; Housing; Image; Imaging technology; Immunoelectron Microscopy; Individual; Instruction; Label; Laboratories; Lasers; Learning; Life; Light; Lighting; Mechanics; Metals; Microscope; Microscopy; Microtomy; NCI Center for Cancer Research; Negative Staining; Noise; Optics; Phase; Photons; Positioning Attribute; Preparation; Procedures; Proteins; Research; Research Personnel; Resolution; Resource Sharing; Resources; Sampling; Scanning; Science; Scientist; Services; Shadowing (Histology); Signal Transduction; Speed; Staging; Structure; System; Three-Dimensional Imaging; Time; Transmission Electron Microscopy; Vacuum; bioimaging; cell fixing; cellular imaging; design; fluorescence microscope; insight; instrumentation; member; programs; research study; square foot; transmission process

PROJECT SUMMARY (See instructions): The Microscopy Shared Resource was developed in concert with the initiation of the Cold Spring Harbor Laboratory Cancer Center in 1986 and has been continuously serving Cancer Center members since that time. The Resource provides core services to scientists engaged in a wide range of scientific endeavors. Among the types of services rendered are wide-field fluorescence microscopy, deconvolution microscopy, confocal microscopy including point-scanning and spinning disk, live cell imaging, super-resolution structured illumination microscopy, standard transmission electron microscopy, immunoelectron microscopy (enzymatic and gold labeling), DNA spreads and evaluation of subcellular fractionation procedures by negative staining or thin sectioning. All of these services are highly technical and labor intensive. Without the Microscopy Shared Resource it would be extremely difficult for individual investigators to have access to this instrumentation and these types of services, which are critical to their research programs. In addition, the Shared Resource serves as a central focal point where users can interact, learn about new imaging technologies, discuss availability of fluorescent proteins or antibodies, and gain insight into imaging approaches that will advance their science. Over the past year CSHL has acquired support to purchase 3 new microscope systems (Zeiss LSM710 confocal, Perkin Elmer UltraVIEW VoX confocal, and Applied Precision OMX super-resolution microscope). The resource currently maintains nine microscope systems, each with unique features, providing the user group with a complete battery of state-of-the-art instrumentation and technical support.

项目总结（见说明）： 显微镜共享资源是在1986年冷泉港实验室癌症中心成立时开发的，自那时以来一直为癌症中心成员提供服务。该资源为从事广泛科学工作的科学家提供核心服务。 所提供的服务类型包括宽视场荧光显微镜、去卷积显微镜、包括点扫描和旋转盘的共聚焦显微镜、活细胞成像、超分辨率结构照明显微镜、标准透射电子显微镜、免疫电子显微镜（酶促）、荧光显微镜和荧光显微镜。 和金标记）、DNA扩散和通过负染色或薄切片进行亚细胞分级分离程序的评价。所有这些服务都是高度技术性和劳动密集型的。如果没有显微镜共享资源，个体研究者将很难获得这种仪器和这些类型的服务，这对他们的研究计划至关重要。此外，共享资源作为一个中心焦点，用户可以互动，了解新的成像技术，讨论荧光蛋白或抗体的可用性，并深入了解成像方法，这将促进他们的科学。在过去的一年中，CSHL已经获得了购买3个新显微镜系统的支持（蔡司LSM710共聚焦，珀金埃尔默UltraVIEW VoX共聚焦和应用精密OMX超分辨率显微镜）。该资源目前维护着9个显微镜系统， 每个都具有独特的功能，为用户群提供一整套最先进的仪器和技术支持。