Microscopy

项目总结(见说明)： 显微镜共享资源是与冷泉港实验室癌症中心于1986年成立时共同开发的，自那时以来一直为癌症中心成员提供服务。该资源为从事广泛科学工作的科学家提供核心服务。 提供的服务类型包括广域荧光显微镜、去卷积显微镜、包括点扫描和旋转圆盘的共聚焦显微镜、活细胞成像、超分辨率结构照明显微镜、标准透射电子显微镜、免疫电子显微镜(酶 和金标记法)、DNA扩散和通过阴性染色或薄片对亚细胞分离过程的评价。所有这些服务都是高度技术性和劳动密集型的。如果没有显微镜共享资源，单个研究人员将极难访问这种仪器和这些类型的服务，这对他们的研究计划至关重要。此外，共享资源是一个中心焦点，用户可以在这里进行互动，了解新的成像技术，讨论荧光蛋白或抗体的可用性，并深入了解将促进他们的科学的成像方法。在过去的一年里，CSHL获得了购买3个新显微镜系统的支持(蔡司LSM710共焦显微镜、Perkin Elmer UltraView Vox共焦显微镜和应用精密OMX超分辨率显微镜)。该资源目前维护着9个显微镜系统， 每个都有独特的功能，为用户群体提供了一整套最先进的仪器设备和技术支持。

PROJECT SUMMARY (See instructions): The Microscopy Shared Resource was developed in concert with the initiation of the Cold Spring Harbor Laboratory Cancer Center in 1986 and has been continuously serving Cancer Center members since that time. The Resource provides core services to scientists engaged in a wide range of scientific endeavors. Among the types of services rendered are wide-field fluorescence microscopy, deconvolution microscopy, confocal microscopy including point-scanning and spinning disk, live cell imaging, super-resolution structured illumination microscopy, standard transmission electron microscopy, immunoelectron microscopy (enzymatic and gold labeling), DNA spreads and evaluation of subcellular fractionation procedures by negative staining or thin sectioning. All of these services are highly technical and labor intensive. Without the Microscopy Shared Resource it would be extremely difficult for individual investigators to have access to this instrumentation and these types of services, which are critical to their research programs. In addition, the Shared Resource serves as a central focal point where users can interact, learn about new imaging technologies, discuss availability of fluorescent proteins or antibodies, and gain insight into imaging approaches that will advance their science. Over the past year CSHL has acquired support to purchase 3 new microscope systems (Zeiss LSM710 confocal, Perkin Elmer UltraVIEW VoX confocal, and Applied Precision OMX super-resolution microscope). The resource currently maintains nine microscope systems, each with unique features, providing the user group with a complete battery of state-of-the-art instrumentation and technical support.