MicroArray

微阵列

• 批准号: 8340293
• 负责人: BRUCE W. STILLMAN
• 金额: $ 20.67万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-17 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340293
• 关键词: Bioinformatics; Biological; Cancer Center; Cancer Center at Cold Spring Harbor Laboratory; Candidate Disease Gene; Complement; Copy Number Polymorphism; DNA Sequencing Shared Resource; DNA-Protein Interaction; Data; Evolution; Fluorescent in Situ Hybridization; Gene Expression; Gene Expression Profile; Gene Targeting; Genes; Genetic; Genome; Genomics; Grant; Human; Individual; Instruction; Libraries; Maps; Methodology; Methods; Microarray Analysis; Microarray Shared Resource; Molecular Profiling; Oligonucleotide Microarrays; Oncogenes; Pathway Analysis; Pattern; Printing; RNA; Resolution; Resource Sharing; Resources; Sampling; Sampling Studies; Screening procedure; Services; Surveys; Techniques; Technology; Time; Update; Variant; analog; base; cDNA Arrays; cell type; chromatin immunoprecipitation; chromatin modification; cost; cost effective; gene discovery; genome sequencing; genome-wide; insight; member; multidisciplinary; new technology; novel; programs; research study; tool

PROJECT SUMMARY (See instructions): The Microarray Shared Resource allows the CSHL Cancer Center members to take advantage of powerful genomics tools and techniques. The Shared Resource provides full service microarray analysis, primarily focusing on the Affymetrix and Agilent platforms. The microarray applications used by the facility provide RNA profiling, genome analysis of copy number variation and insights into DNA/protein interactions and chromatin modifications by means of chromatin immunoprecipitation. The use of Microarray services has promoted and strengthened multidisciplinary interactions with the Bioinformatics and DNA Sequencing Shared Resources. Microarrays provide a low resolution, genome-wide view of changes in gene expression in different cell types or under different conditions, which serve as a starting point for pathway analysis and the identification of key regions of variation in cancer gene discovery experiments. In many cases, microarray experiments can be a prelude to high throughput sequencing and can help focus the sequencing efforts.

项目总结（见说明）： 微阵列共享资源允许CSHL癌症中心成员利用强大的基因组学工具和技术。共享资源提供全方位的微阵列分析服务，主要集中在Affyoung和Agilent平台上。该设施使用的微阵列应用程序提供RNA分析，拷贝数变异的基因组分析和DNA/蛋白质相互作用的见解， 染色质修饰通过染色质免疫沉淀。微阵列服务的使用促进和加强了与生物信息学和DNA测序共享资源的多学科互动。微阵列提供了不同细胞类型或不同条件下基因表达变化的低分辨率全基因组视图，其作为途径分析的起点， 在癌症基因发现实验中识别关键变异区域。在许多情况下，微阵列实验可以是高通量测序的前奏，并且可以帮助集中测序工作。