Acquisition of an Orbitrap Elite Mass Spectrometer with ETD

购买带 ETD 的 Orbitrap Elite 质谱仪

• 批准号: 8447748
• 负责人: Beatrix Magdalena Ueberheide
• 金额: $ 60万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447748
• 关键词: Biomedical Research; Bladder; Chromatin; Cities; Commit; Communities; Complex Mixtures; DNA biosynthesis; Dissociation; Electron Transport; Funding; Housing; Maps; New York; Pathway interactions; Peptides; Post-Translational Protein Processing; Proteins; Proteomics; Regulation; Research; Research Infrastructure; Resources; Source; Stem cells; Technology; Ubiquitin; Universities; Venoms; Work; college; drug development; improved; mass spectrometer; medical schools; multidisciplinary; protein complex; transcription factor; tumor progression

DESCRIPTION (provided by applicant): The new Proteomics Resource Core at the NYU School of Medicine is committed to provide the biomedical research community access to state of the art mass spectrometric technology to facilitate their research and improve the scientific infrastructure. We are therefore requesting funds for an Orbitrap Elite mass spectrometer with Electron Transfer Dissociation (ETD) from Thermo Fisher Scientific to enable high throughput proteomic studies for the identification and quantification of proteins in complex mixtures, characterization of protein complexes, mapping of post translational modifications, as well as in depth structural characterization of proteins and peptides. We have assembled a group of multidisciplinary users at NYU and at Hunter College (City University of New York) whose work on mechanisms of cancer progression through over- or inactivation of the ubiquitin pathway, stem cell regulation, DNA replication control, transcription factor regulation, chromatin regulation, bladder urothelial formation, as well as drug development using natural sources (venoms) will be impossible without an in-house state of the art mass spectrometer.

描述（由申请人提供）：纽约大学医学院新的蛋白质组学资源核心致力于为生物医学研究界提供最先进的质谱技术，以促进他们的研究和改善科学基础设施。因此，我们向赛默飞世尔科学公司申请资金，用于高通量蛋白质组学研究，用于复杂混合物中蛋白质的鉴定和定量，蛋白质复合物的表征，翻译后修饰的绘制，以及蛋白质和肽的深度结构表征。我们在纽约大学和亨特学院（纽约城市大学）召集了一组多学科的用户，他们通过泛素途径的过度或失活，干细胞调节，DNA复制控制，转录因子调节，染色质调节，膀胱尿路上皮形成以及使用天然来源（毒液）的药物开发来研究癌症进展机制，如果没有内部最先进的质谱仪，这些工作将是不可能的。

项目成果

An allosteric inhibitor of bacterial Hsp70 chaperone potentiates antibiotics and mitigates resistance.

• DOI: 10.1016/j.chembiol.2021.11.004
• 发表时间: 2022-05-19
• 期刊: CELL CHEMICAL BIOLOGY
• 影响因子: 8.6
• 作者: Hosfelt, Jordan;Richards, Aweon;Zheng, Meng;Adura, Carolina;Nelson, Brock;Yang, Amy;Fay, Allison;Resager, William;Ueberheide, Beatrix;Glickman, J. Fraser;Lupoli, Tania J.
• 通讯作者: Lupoli, Tania J.