Development of nonhuman primate reference transcriptome resources
非人灵长类参考转录组资源的开发
基本信息
- 批准号:8494707
- 负责人:
- 金额:$ 73.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-09-01 至 2015-06-30
- 项目状态:已结题
- 来源:
- 关键词:AIDS VaccinesAcquired Immunodeficiency SyndromeAcuteAmino Acid SequenceAnimalsArchivesAtlasesBinding SitesBioinformaticsBiologyBiomedical ResearchChargeCodeCommunitiesComparative BiologyComplementDataData AnalysesData SetDatabasesDepositionDevelopmentDevelopmental ProcessDiseaseDrug TargetingEvolutionExonsFundingGene ExpressionGene Expression ProfileGene ProteinsGenesGeneticGenetic TranscriptionGenomeGenomicsGoalsHealthHumanImmune responseInfectionInflammatoryIntronsInvestigationLettersLightMacacaMacaca mulattaMicroRNAsModelingMonoclonal Antibody R24National Center for Research ResourcesNatural ImmunityPeptide Sequence DeterminationPhasePrimatesPrincipal InvestigatorProcessProteinsProteomicsRNARNA Sequence AnalysisReadingRegulationResearchResearch ActivityResearch PersonnelResource DevelopmentResourcesRoleSIVSamplingScientistStagingStructureSystems BiologyTechnologyTissue SampleTissuesTranscriptTranslational ResearchUntranslated RNAVaccine ResearchVariantVirus DiseasesWorkcomputerized data processingcostdesigngenome sequencinghuman diseasenext generation sequencingnonhuman primatenovel vaccinesprogramsresearch studyresponsetooltool developmentvirus host interactionweb siteworking group
项目摘要
DESCRIPTION (provided by applicant): We propose to generate comprehensive nonhuman primate (NHP) reference transcriptome databases using lllumina's next-generation sequencing platform. These databases will contain information on protein-coding, long noncoding, and small noncoding transcripts, and will provide vast amounts of new information regarding gene and transcript structure, expression, and regulation. To enhance this resource, we will also generate a tissue-specific transcriptome atlas for rhesus macaque and whole transcriptome information from a NHP model of AIDS. These databases will serve as a resource for scientists working on evolution, comparative biology, genetics, genomics, and NHP models of human disease. In Aim 1, we will collaborate with lllumina scientists to generate whole transcriptome (protein-coding and long noncoding) information for 14 NHP species/subspecies, lllumina will perform this sequencing at no cost to the application. The majority of these NHP genomes will be sequenced within the year, and transcriptome information will facilitate genome assembly, annotation, development of new tools for systems biology research, while also providing broad resources for evolutionary biologists. To complement the whole transcriptome project, we will use our lllumina Genome Analyzer to generate a tissue specific transcriptome for rhesus macaque, the most widely used NHP in biomedical research. In Aim 2, we will sequence protein-coding, long noncoding, and small noncoding transcripts from an independently funded study focused on a NHP acute infection model of AIDS. The resulting data will expand the NHP reference transcriptome databases to include new information on virus-host interactions, innate immunity, inflammatory processes, the transition to the adaptive immune response, and infection-induced transcripts. Finally, whole transcriptome data from both Aims will enable the discovery and quantification of noncoding RNAs, which have widespread functionality but are not well understood. Given the importance of NHP primates as models for human health and disease, it is imperative that whole transcriptome information be available for these animals.
描述(由申请人提供):我们提出使用Illumina的下一代测序平台生成全面的非人灵长类动物(NHP)参考转录组数据库。这些数据库将包含有关蛋白质编码、长非编码和小非编码转录本的信息,并将提供有关基因和转录本结构、表达和调控的大量新信息。为了增强这一资源,我们还将生成恒河猴的组织特异性转录组图谱和来自艾滋病NHP模型的全转录组信息。这些数据库将作为研究进化、比较生物学、遗传学、基因组学和人类疾病NHP模型的科学家的资源。在Aim 1中,我们将与Illumina科学家合作,为14个NHP物种/亚种生成全转录组(蛋白质编码和长非编码)信息,Illumina将免费进行此测序。这些NHP基因组中的大多数将在年内测序,转录组信息将促进基因组组装,注释,开发系统生物学研究的新工具,同时也为进化生物学家提供广泛的资源。为了补充整个转录组项目,我们将使用我们的Illumina基因组分析仪为恒河猴生成组织特异性转录组,这是生物医学研究中使用最广泛的NHP。在目标2中,我们将对来自一项独立资助的研究的蛋白质编码、长非编码和小非编码转录本进行测序,该研究的重点是艾滋病的NHP急性感染模型。由此产生的数据将扩展NHP参考转录组数据库,以包括有关病毒-宿主相互作用,先天免疫,炎症过程,向适应性免疫反应过渡以及感染诱导转录的新信息。最后,来自两个目标的全转录组数据将使非编码RNA的发现和定量成为可能,这些RNA具有广泛的功能,但尚未得到很好的理解。鉴于NHP灵长类动物作为人类健康和疾病模型的重要性,必须为这些动物提供完整的转录组信息。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MICHAEL G KATZE其他文献
MICHAEL G KATZE的其他文献
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{{ truncateString('MICHAEL G KATZE', 18)}}的其他基金
Systems Biology of Infectious Diseases: Pathogenesis to Personalized Medicine
传染病的系统生物学:个性化医疗的发病机制
- 批准号:
8789251 - 财政年份:2014
- 资助金额:
$ 73.16万 - 项目类别:
Nonhuman Primate Core Functional Genomics Laboratory for AIDS Vaccines Research a
非人类灵长类艾滋病疫苗研究核心功能基因组学实验室
- 批准号:
8748807 - 财政年份:2013
- 资助金额:
$ 73.16万 - 项目类别:
Development of nonhuman primate reference transcriptome resources
非人灵长类参考转录组资源的开发
- 批准号:
8690994 - 财政年份:2011
- 资助金额:
$ 73.16万 - 项目类别:
Development of nonhuman primate reference transcriptome resources
非人灵长类参考转录组资源的开发
- 批准号:
8147510 - 财政年份:2011
- 资助金额:
$ 73.16万 - 项目类别:
IMMUNOLOGICAL RESPONSE TO INFLUENZA INFECTION IN NH PRIMATES
NH 灵长类动物对流感感染的免疫反应
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8357600 - 财政年份:2011
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$ 73.16万 - 项目类别:
Development of nonhuman primate reference transcriptome resources
非人灵长类参考转录组资源的开发
- 批准号:
8325121 - 财政年份:2011
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PNNL PROTEOMICS AND THE UW P51 PRIMATE GENOME CENTER
PNNL 蛋白质组学和华盛顿大学 P51 灵长类基因组中心
- 批准号:
8365475 - 财政年份:2011
- 资助金额:
$ 73.16万 - 项目类别:
Systems Biology Approaches to Viral Pathogenesis and Immunity
病毒发病机制和免疫的系统生物学方法
- 批准号:
8234065 - 财政年份:2011
- 资助金额:
$ 73.16万 - 项目类别:
IMMUNOLOGICAL RESPONSE TO PRIMARY & SECONDARY INFLUENZA INFECTION IN NH PRIMATES
原发性免疫反应
- 批准号:
8172762 - 财政年份:2010
- 资助金额:
$ 73.16万 - 项目类别:
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