DEVELOPMENT OF A PRIMATE GENOMICS RESOURCE

灵长类基因组资源的开发

• 批准号: 8357630
• 负责人: MICHAEL G KATZE
• 金额: $ 37.79万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357630
• 关键词: Acquired Immunodeficiency Syndrome; Acute; Animal Experimentation; Animals; Communities; Complement; Databases; Development; Event; Funding; Genomics; Goals; Grant; Immune response; Individual; Infection; Inflammatory; Internet; Intervention; Macaca; Macaca mulatta; Mass Spectrum Analysis; Modality; Modeling; National Center for Research Resources; Natural Immunity; Outcome; Pathway interactions; Primates; Principal Investigator; Process; Proteins; Proteomics; Protocols documentation; Research; Research Infrastructure; Research Personnel; Resolution; Resources; SIV; Source; System; Systems Biology; Technology; Tissues; United States National Institutes of Health; Vaccines; Virus Diseases; cost; functional genomics; male; nonhuman primate; protein profiling; rectal; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary goal of the model remains the same as in the original protocol. This project is focused on creating genomic/proteomic resources that can be applied to research using nonhuman primates. Through the construction of proteomic resources, we intend to provide the research community with the full complement of species-specific genomic and proteomic tools needed to apply the technologies of functional genomics to nonhuman primate research. We will use sensitive, high-resolution mass spectrometry to build databases of identified proteins present in various rhesus macaque (Macaca mulatta) tissues. These databases will be used to characterize the changes in cellular protein profiles that occur in response to simian immunodeficiency virus infection. These studies will allow us to evaluate these resources in an experimental system that will enhance the use of macaques as models for AIDS research. The results will be shared via Web portals, as a resource to enable researchers to maximize the types and amounts of information that can be obtained from these valuable research animals. We will perform a systems biology interrogation of an acute mucosal infection model, employing intra-rectal challenge with SIVmac251 in 24 male, Indian-origin rhesus macaques. The primary hypothesis is that a detailed systems level analysis of early changes in the acute infection with a pathogenic strain of SIV will reveal events in innate immunity, inflammatory processes, and the ensuing transition to the adaptive immune response. As one corollary to this hypothesis, these features may serve as prognosticators of outcome, as reflected in the variability of individual animals. A second corollary is that such a systems level analysis will identify specific targets and pathways for intervention by pharmacologic agents or vaccine modalities

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该模型的主要目标与原始方案相同。该项目的重点是创建基因组/蛋白质组资源，可应用于使用非人灵长类动物的研究。通过蛋白质组资源的建设，我们打算为研究界提供所需的物种特异性基因组和蛋白质组学工具的完整补充，将功能基因组学技术应用于非人灵长类动物的研究。我们将使用灵敏的高分辨率质谱法来建立各种恒河猴（Macaca mulatta）组织中已鉴定蛋白质的数据库。这些数据库将被用来表征细胞蛋白质谱的变化，发生在响应猿猴免疫缺陷病毒感染。这些研究将使我们能够在一个实验系统中评估这些资源，这将提高猕猴作为艾滋病研究模型的使用。结果将通过门户网站共享，作为一种资源，使研究人员能够最大限度地从这些有价值的研究动物中获得信息的类型和数量。 我们将对24只雄性印度恒河猴进行SIVmac 251直肠内激发，对急性粘膜感染模型进行系统生物学研究。 主要的假设是，一个详细的系统水平分析的早期变化，在急性感染的致病株SIV将揭示先天免疫，炎症过程中的事件，并随后过渡到适应性免疫反应。作为这一假设的一个推论，这些特征可以作为结果的预测因子，反映在个体动物的变异性中。第二个推论是，这种系统水平的分析将确定特定的目标和途径的干预药物或疫苗的方式