The Comprehensive Center for Chemical Probe Discovery and Optimization at Scripps

斯克里普斯化学探针发现和优化综合中心

基本信息

  • 批准号:
    8525448
  • 负责人:
  • 金额:
    $ 786.6万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-09-01 至 2015-05-31
  • 项目状态:
    已结题

项目摘要

High Throughput Screening (HTS) leverages automation, chemistry, biology and information technologies to rapidly assay the pharmacologic activity of drug-like molecules. As applied to drug discovery, it has been successful for discovering the precursors of marketed drugs as well as probes of various cellular and biochemical pathways. Scripps Florida is the home of the ¿ultra¿ HTS (uHTS) Implementation core of the Scripps Research Institute Molecular Screening Center (SRIMSC). Core staff are responsible for developing and implementing the SRIMSC¿s screening assays, managing the Molecular Libraries Small Molecule Repository (MLSMR) screening collection of >360,000 samples, screening the MLSMR library at a throughput in excess of 1 million samples per day, quality control & reporting of all screening results to the NIH¿s publically available database (PubChem), cheminformatics support and overall project management for the SRIMSC. The core sustains full production capabilities for the NIH by screening the entire MLSMR compound library against more than 25 unique targets per year. As of this writing core staff have completed more than 185 high throughput screening campaigns for 175 molecular targets, with more than 40 million wells tested. Staff also execute lower-throughput bioassays to support the SRIMSC¿s hit-to-probe medicinal chemistry efforts, participating in more than 50 probe development projects per year. Target classes screened by the core include receptors, transcription factors, transferases, hydrolases, oxidoreductases, lyases, isomerases and ligases; protein interactions (protein-protein, protein-DNA, and protein-RNA) and phenotypic bioassays. Targets have been purified for screening (e.g. enzymes or proteins) or assayed in whole-cell mammalian, bacterial, insect, yeast/fungal, and parasite systems. Screening formats include biochemical enzymatic & binding affinity assays, as well as whole-cell reporter gene, second messenger, membrane potential, ion flux, complementation, protein conformation, renaturation and toxicity assays. A variety of plate-based detection formats including kinetic (FLIPR, Alphascreen, TR-FRET, FP etc.) and high content analysis (HCA) are utilized for screening efforts. More information can be found at: http://hts.florida.scripps.edu/
高通量筛选(HTS)利用自动化、化学、生物学和信息技术来快速检测类药物分子的药理活性。作为药物发现的应用,它已经成功地发现了上市药物的前体以及各种细胞和生化途径的探针。佛罗里达州斯克里普斯是斯克里普斯研究院分子筛选中心(SRIMSC)超高温超导(UHTS)实施核心的所在地。核心员工负责开发和实施S筛选方法,管理360,000个样本的分子图书馆小分子资料库筛选收集,以每天超过100万个样本的吞吐量筛选分子小分子资料库,质量控制和将所有筛选结果报告给国家卫生研究院S公开数据库(PubChem),化学信息学支持和国家卫生研究院S公共可用数据库(PubChem)的整体项目管理。 该核心通过对整个MLSMR化合物库进行筛选,以每年超过25个独特的目标来维持NIH的全部生产能力。在撰写本文时,核心员工已经为175个分子靶标完成了185多个高通量筛选活动,测试了4000多万口井。工作人员还执行较低吞吐量的生物检测,以支持国家药品监督管理委员会S点击-探针药物化学的努力,每年参与50多个探针开发项目。 核心筛选的靶类包括受体、转录因子、转移酶、水解酶、氧化还原酶、裂解酶、异构酶和连接酶;蛋白质相互作用(蛋白质-蛋白质、蛋白质-DNA和蛋白质-RNA)和表型生物测定。在哺乳动物、细菌、昆虫、酵母/真菌和寄生虫系统中对目标进行纯化以进行筛选(例如,酶或蛋白质)或进行检测。筛选方法包括生化、酶和结合亲和力分析,以及全细胞报告基因、第二信使、膜电位、离子通量、互补性、蛋白质构象、复性和毒性分析。多种平板检测格式,包括KYNIC(FLIPR、Alphascreen、TR-FRET、FP等)和高含量分析(HCA)用于筛选工作。欲了解更多信息,请访问:http://hts.florida.scripps.edu/。

项目成果

期刊论文数量(39)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Identification of Potent and Selective Inhibitors of the Plasmodium falciparum M18 Aspartyl Aminopeptidase (PfM18AAP) of Human Malaria via High-Throughput Screening.
  • DOI:
    10.1177/1087057114525852
  • 发表时间:
    2014-08
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Spicer T;Fernandez-Vega V;Chase P;Scampavia L;To J;Dalton JP;Da Silva FL;Skinner-Adams TS;Gardiner DL;Trenholme KR;Brown CL;Ghosh P;Porubsky P;Wang JL;Whipple DA;Schoenen FJ;Hodder P
  • 通讯作者:
    Hodder P
A high-throughput screening compatible assay for activators and inhibitors of methionine sulfoxide reductase A.
  • DOI:
    10.1089/adt.2009.0263
  • 发表时间:
    2010-10
  • 期刊:
  • 影响因子:
    1.8
  • 作者:
    David Brunell;H. Weissbach;P. Hodder;N. Brot
  • 通讯作者:
    David Brunell;H. Weissbach;P. Hodder;N. Brot
An altered zinc-binding site confers resistance to a covalent inactivator of New Delhi metallo-beta-lactamase-1 (NDM-1) discovered by high-throughput screening.
  • DOI:
    10.1016/j.bmc.2013.03.031
  • 发表时间:
    2013-06-01
  • 期刊:
  • 影响因子:
    3.5
  • 作者:
    Thomas, Pei W.;Spicer, Timothy;Cammarata, Michael;Brodbelt, Jennifer S.;Hodder, Peter;Fast, Walter
  • 通讯作者:
    Fast, Walter
Preparation of tetrasubstituted pyrimido[4,5-d]pyrimidine diones.
四取代嘧啶并[4,5-d]嘧啶二酮的制备。
  • DOI:
    10.1016/j.tetlet.2015.02.051
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    1.8
  • 作者:
    Wang,Hui;Wang,Chao;Bannister,ThomasD
  • 通讯作者:
    Bannister,ThomasD
Modulators of the Sphingosine 1-phosphate receptor 1.
  • DOI:
    10.1016/j.bmcl.2013.09.058
  • 发表时间:
    2013-12-01
  • 期刊:
  • 影响因子:
    2.7
  • 作者:
    Urbano, Mariangela;Guerrero, Miguel;Rosen, Hugh;Roberts, Edward
  • 通讯作者:
    Roberts, Edward
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

HUGH ROSEN其他文献

HUGH ROSEN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('HUGH ROSEN', 18)}}的其他基金

Optimization of S1P3 antagonists for fibrotic disease
用于纤维化疾病的 S1P3 拮抗剂的优化
  • 批准号:
    9252358
  • 财政年份:
    2016
  • 资助金额:
    $ 786.6万
  • 项目类别:
Administration and Mgmt (California)
行政和管理(加利福尼亚州)
  • 批准号:
    8538720
  • 财政年份:
    2012
  • 资助金额:
    $ 786.6万
  • 项目类别:
HTS for inhibitors of NADPH Oxidase 2 (NOX 2)
NADPH 氧化酶 2 (NOX 2) 抑制剂的 HTS
  • 批准号:
    7991279
  • 财政年份:
    2010
  • 资助金额:
    $ 786.6万
  • 项目类别:
Project 3
项目3
  • 批准号:
    8152435
  • 财政年份:
    2010
  • 资助金额:
    $ 786.6万
  • 项目类别:
Administration and Mgmt (California)
行政和管理(加利福尼亚州)
  • 批准号:
    8120933
  • 财政年份:
    2010
  • 资助金额:
    $ 786.6万
  • 项目类别:
The Comprehensive Center for Chemical Probe Discovery and Optimization at Scripps
斯克里普斯化学探针发现和优化综合中心
  • 批准号:
    7945401
  • 财政年份:
    2008
  • 资助金额:
    $ 786.6万
  • 项目类别:
The Comprehensive Center for Chemical Probe Discovery and Optimization at Scripps
斯克里普斯化学探针发现和优化综合中心
  • 批准号:
    7682882
  • 财政年份:
    2008
  • 资助金额:
    $ 786.6万
  • 项目类别:
The Comprehensive Center for Chemical Probe Discovery and Optimization at Scripps
斯克里普斯化学探针发现和优化综合中心
  • 批准号:
    8538719
  • 财政年份:
    2008
  • 资助金额:
    $ 786.6万
  • 项目类别:
The Comprehensive Center for Chemical Probe Discovery and Optimization at Scripps
斯克里普斯化学探针发现和优化综合中心
  • 批准号:
    8334811
  • 财政年份:
    2008
  • 资助金额:
    $ 786.6万
  • 项目类别:
Administration and Mgmt (California)
行政和管理(加利福尼亚州)
  • 批准号:
    8332830
  • 财政年份:
    2008
  • 资助金额:
    $ 786.6万
  • 项目类别:

相似海外基金

Treecle - data and automation to unlock woodland creation in the UK to achieve net zero
Treecle - 数据和自动化解锁英国林地创造以实现净零排放
  • 批准号:
    10111492
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    SME Support
STTR Phase II: Optimized manufacturing and machine learning based automation of Endothelium-on-a-chip microfluidic devices for drug screening applications.
STTR 第二阶段:用于药物筛选应用的片上内皮微流体装置的优化制造和基于机器学习的自动化。
  • 批准号:
    2332121
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Cooperative Agreement
Improving access to AI automation to support new digital offerings within Professional/Financial Services
改善对人工智能自动化的访问,以支持专业/金融服务中的新数字产品
  • 批准号:
    10095096
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Collaborative R&D
Cost-Effective, AI-driven Automation Technology for Cell Culture Monitoring: Boosting Efficiency and Sustainability in Industrial Biomanufacturing and Streamlining Supply Chains
用于细胞培养监测的经济高效、人工智能驱动的自动化技术:提高工业生物制造的效率和可持续性并简化供应链
  • 批准号:
    10104748
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Launchpad
Sustainable Remanufacturing solution with increased automation and recycled content in laser and plasma based process (RESTORE)
可持续再制造解决方案,在基于激光和等离子的工艺中提高自动化程度和回收内容(RESTORE)
  • 批准号:
    10112149
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    EU-Funded
Next-generation automation and PAT implementation for QbD and enhanced approaches for cell and gene therapy
QbD 的下一代自动化和 PAT 实施以及细胞和基因治疗的增强方法
  • 批准号:
    10087446
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Collaborative R&D
SBIR Phase II: Radar-based Building Automation
SBIR 第二阶段:基于雷达的楼宇自动化
  • 批准号:
    2335079
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Cooperative Agreement
Automation and cost reduction of the hardware and software components of a novel indoor sustainable vertical growing solution
新型室内可持续垂直种植解决方案的硬件和软件组件的自动化和成本降低
  • 批准号:
    83007861
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Innovation Loans
Artificial intelligence coupled to automation for accelerated medicine design
人工智能与自动化相结合,加速药物设计
  • 批准号:
    EP/Z533038/1
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Research Grant
Mighty Accounting - Accountancy Automation for 1-person limited companies.
Mighty Accounting - 1 人有限公司的会计自动化。
  • 批准号:
    10100360
  • 财政年份:
    2024
  • 资助金额:
    $ 786.6万
  • 项目类别:
    Collaborative R&D
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了