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BIOMIMETIC SCAFFOLD FOR BONE-REPAIR

用于骨修复的仿生支架

基本信息

批准号：
7218004
负责人：
Anne George
金额：
$ 30.9万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7218004
关键词：
3-Dimensional Adhesives Alkaline Phosphatase Apatites Area Biocompatible Biocompatible Materials Biodegradation Biological Biological Process Biomimetics Blood Vessels Bone Matrix Bone Regeneration Bone Tissue C-terminal Cell Adhesion Cell surface Cells Characteristics Circular Dichroism Clinical Collagen Collagen Type I Condition Confocal Microscopy Defect Dental Dentin Deposition Diagnostic radiologic examination Differentiation and Growth Electron Microscopy Embryo Engineering Environment Extracellular Matrix Extracellular Matrix Proteins Facility Construction Funding Category Fibronectins Flow Cytometry Fracture Genes Genetic Engineering Goals Hydrogels Hydroxyapatites Immunoblotting Implant In Vitro Inflammatory Integrins Ionic Strengths Kinetics Leucine Zippers Life Expectancy Ligands Light Mechanics Mediating Mediator of activation protein Mesenchymal Methodology Methods Minerals Modeling Molecular Structure Morphology Natural regeneration Nature Nodule Northern Blotting Nude Rats Numbers Organ Transplantation Orthopedics Osteoblasts Osteocalcin Osteogenesis Osteoid Peptides Performance Personal Satisfaction Physiological Play Population Porosity Process Property Proteins RGD (sequence)Rattus Resolution Reverse Transcriptase Polymerase Chain Reaction Role Route Scanning Electron Microscopy Scanning Transmission Electron Microscopy Procedures Signal Pathway Signal Transduction Skeletal system Spatial Distribution Staining method Stains Techniques Temperature Testing Texture Time Tissue Engineering Tissues Tooth structure Transmission Electron Microscopy Trauma Trichrome stain Week Wound Healing base biomaterial compatibility biomaterial development biomineralization bone bone sialoprotein bone strength density dentin matrix protein 1 design extracellular implantation in vivo light scattering mineralization nanoindentation nanomaterials novel novel strategies polypeptide protein aminoacid sequence receptor repaired research study response restoration scaffold self assembly subcutaneous three dimensional structure tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): Biomineralization processes such as formation of bones and teeth require controlled mineral deposition and self-assembly into hierarchical biocomposites with unique mechanical properties. Ideal biomaterials for regeneration and repair of intramembranous and endochondral bone must be biocompatible, an optimal template for vascular invasion, facilitate normal differentiation of osteoprogenitor cells, ideal for deposition of mineral and possess intrinsic biocompatible properties. Novel methods of designing tissue repair and restoration materials using bioinspired strategies are currently in vogue. Therefore, it is possible that hierarchically organized nanomaterials can be constructed by tailor-made peptide sequences with self-assembling properties. Our overall goal is to employ novel strategies for fabrication of biomaterial for bone repair with a three-dimensional microstructure, by incorporating peptides with self-assembly characteristics along with spatial presentation of bioactive ligands that would provide a physiological environment for cells, facilitating proliferation, differentiation and inducing mineralized tissue formation. Specifically we will: (1) Design and fabricate self-assembled matrices that contain motifs for self-assembly, cell-adhesion and mineral induction; (2) Test the functionality's of the motifs incorporated in the scaffold (3) Test the osteogenic activity of the scaffold in vivo.

描述（由申请人提供）：生物矿化过程（如骨骼和牙齿的形成）需要受控的矿物沉积和自组装成具有独特机械性能的分层生物复合材料。用于膜内和软骨内骨再生和修复的理想生物材料必须具有生物相容性、血管侵入的最佳模板、促进骨祖细胞的正常分化、理想的矿物质沉积以及具有内在的生物相容性。使用生物启发策略设计组织修复和恢复材料的新方法目前很流行。因此，有可能通过定制具有自组装特性的肽序列来构建分级组织的纳米材料。我们的总体目标是采用新的策略，通过将具有自组装特性的肽沿着与生物活性配体的空间呈现，为细胞提供生理环境，促进增殖，分化和诱导矿化组织形成，来制造具有三维微结构的骨修复生物材料。具体而言，我们将： (1)设计和制造自组装矩阵，包含自组装，细胞粘附和矿物质诱导的基序; (2)测试纳入支架的基序的功能 (3)测试支架的体内成骨活性。