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Revealing the connectivity and functionality of brain stem circuits

揭示脑干回路的连接性和功能

基本信息

批准号：
8827174
负责人：
Darwin K BERG
金额：
$ 85.16万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-30 至 2017-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8827174
关键词：
Area Atlases Automobile Driving Behavior Behavior Control Behavioral Biological Assay Birth Brain Brain Stem Breathing Cell Lineage Cell Nucleus Cells Collaborations Comparative Anatomy Complex Computer Simulation Data Analyses Development Embryonic Development Employee Strikes Engineering Equilibrium Esthesia Face Feedback Functional Imaging Genetic Genetic Programming Group Meetings Image Imagery Individual Interruption Jaw Joints Label Larynx Life Machine Learning Maps Mastication Methods Molecular Molecular Biology Molecular Genetics Motor Motor output Movement Mus Muscle Nature Neck Nervous system structure Neuroanatomy Neurons Neurosciences Nociception Pathway interactions Pattern Phase Physiology Population Procedures Process Publications Recording of previous events Rodent Scheme Sensory Signal Transduction Site Smell Perception Spinal Spinal Cord Synapses System Tactile Taste Perception Technology Theoretical Studies Tongue Touch sensation Trigeminal System Ursidae Family Viral Work base cell type computer studies feeding image processing insight molecular phenotype multidisciplinary neonate nerve stem cell orofacial programs public health relevance tool visual tracking vocalization

项目摘要

DESCRIPTION (provided by applicant): Neuronal circuits in the brainstem control life-sustaining functions, in addition to driving and gating active sensation through taste, smell, and touch. We propose to exploit the advent of molecular and genetic tools to undertake cell lineage marking, cell phenotyping, molecular connectomics, and methods from machine learning and image processing to construct an integrated anatomical and functional atlas of the brainstem. This will enable us to generate anatomical wiring diagrams for the brainstem circuits that control or facial actions. There are three phases to this work. (1) Reveal the identity and organization of brainstem nuclei. Motivated by striking similarities between the developmental plan for the spinal cord and brainstem, we will embrace and extend these efforts to interrogate the molecular composition of neurons that define individual nuclei with sensorimotor circuits in the murine brainstem. (2) Reveal brainstem neuronal circuits and their interactions. We will utilize Tran synaptic viral labeling to delimit pathways from specific muscles that are innervated by facial, trigeminal, hypoglossal, and laryngeal motor nuclei. This will reveal hitherto unknown brainstem circuits, including sites of modulation by higher brain areas. (3) Control the behavior of identified feedback circuits. We will manipulate specific populations of brainstem neurons using a battery of genetic tools to delineate or facial motor actions and motor synergies. The results from the above efforts will be a quantitative map of the functional organization of neurons in the brainstem that enable studies on computations that underlie or facial behavior. An understanding of these fundamental behaviors bears directly on the more general issue of how nervous systems deal with computations that can be performed autonomously, yet must interact synergistically. Thus our proposed program on brainstem circuitry and dynamics will yield general lessons about the nature of neuronal computation. The work performed under this proposal will serve as the basis for a larger national effort in brainstem neuronal computation.