ShEEP Request for Preclinical Micro-Ultrasound Imaging Core

ShEEP 请求临床前微超声成像核心

• 批准号: 9794995
• 负责人: Jeffrey A. Jones
• 金额: --
• 依托单位: RALPH H JOHNSON VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9794995
• 关键词: 3-Dimensional; 4D Imaging; Address; Affect; Agreement; Algorithms; Animal Experimentation; Animal Model; Animals; Area; Behavior assessment; Behavioral; Blood Vessels; Blood flow; Cardiac; Cardiovascular system; Caring; Characteristics; Color; Complement; Computer software; Contrast Media; Core Facility; Data; Disease; Disease Progression; Equipment; Evaluation; Evidence based treatment; Genetic; Genetic Diseases; Geometry; Healthcare; Heart Diseases; Histology; Human; Image; Image Enhancement; Infrastructure; Injury; Intervention; Investigation; Laboratories; Maintenance; Medical; Medical center; Mental disorders; Methods; Microbubbles; Military Personnel; Mission; Modality; Modeling; Molecular; Musculoskeletal; Myocardial; Nature; Neurologic; Orthopedics; Oryctolagus cuniculus; Pathology; Patients; Performance; Physiologic Monitoring; Physiologic pulse; Physiological; Physiology; Research; Research Personnel; Resolution; Services; Sheep; Specimen; Spinal cord injury; Stress; Stroke; System; Techniques; Technology; Three-Dimensional Imaging; Time; Tissue imaging; Tissues; Translating; Traumatic Brain Injury; Ultrasonics; Ultrasonography; Vascular Diseases; Veterans; Work; Zebrafish; animal data; blood flow measurement; cellular imaging; clinical practice; clinically relevant; contrast imaging; cost; design; experience; imaging system; in vivo imaging; instrumentation; novel; operation; pre-clinical; programs; respiratory; surgical research

This proposal addresses a need for the establishment of a Preclinical Micro-Ultrasound Imaging Core. The FujiFilm-VisualSonics Inc. Vevo3100 High Resolution Micro-Ultrasound system provides state-of-the-art imaging that will accelerate research efforts in areas important to the VA medical mission including mental health disorders, traumatic brain injury, spinal cord injury, cardiac disease, orthopedic injury, and stroke. Furthermore, the Preclinical Micro-Ultrasound Imaging Core will complement existing cores (the Small Animal Integrated Behavioral and Physiological Assessment Core, the VA Animal Research Surgical Suite, the VA Cellular and Molecular Evaluation Core, and Histology and Cellular Imaging Core) within the Research Service at the Ralph H. Johnson VA Medical Center in Charleston, SC. We aim to provide state-of-the-art equipment to our investigators, in order to facilitate comprehensive methods of evaluation spanning from the subcellular level to the whole animal. These core facilities, built around key equipment items often costing more than what a single laboratory can provide, have been established by design in order to support and advance our wide-ranging research program that directly impacts the health care of our Veterans. The central hypothesis addressed by this core, is that clinically relevant technology that permits the real-time assessment of disease or injury presence and progression, will enhance the translational significance of the work being done by our VA investigators. This hypothesis will be addressed through 4 Primary Imaging Objectives: 1) Basic imaging of a target tissue using standard ultrasonic techniques with the integration of physiological monitoring (2D, B-mode, M-mode, cardiac/respiratory gating); 2)Advanced algorithms to address tissue specific characteristics like myocardial or vascular wall stress and strain (VevoTrace, VevoStrain, VevoVasc, Speckle tracking); 3) The ability to use novel contrast agents to enhance imaging of tissues, blood flow, or targeted analyte localization (Contrast mode with targeted/non-targeted Microbubble Contrast); and 4) Advanced volumetric imaging of a target tissue in 3D over time (4D imaging) and the ability to integrate color Doppler, power Doppler, and Contrast imaging data. The Vevo3100 High-resolution Micro-Ultrasound Imaging system, including the advanced software packages for detailed quantitative assessment of small animal physiology (blood flow measurements using pulse wave and color Doppler, heart and vascular performance and geometry) using multiple modalities (2D, 3D, and 4D imaging, MicroMarker contrast agents) and can be applied to the genetic-, disease-, and injury- models that are routinely used by our investigators. These assessments are directly applicable to medical issues that represent those affecting our Veterans such as vascular disease, cardiac disease, neurologic illness, musculoskeletal pathologies, stroke, traumatic brain injury, and spinal cord injury. Given the versatility and integrated nature of our core facilities, VA Investigators will be able conduct high-resolution micro-ultrasound imaging of animals, and the data can be used synergistically with other core data, such as that obtained from the physiological and behavior assessment core. The integration of the proposed core with our existing cores allows our researchers to conduct full-service physiological assessments. This represents a unique infrastructure that will significantly advance the research being conducted at our local VAMC. The requested instrumentation will further enhance local VA research capabilities in line with the objectives outlined within the Secretary of the Department of Veterans Affairs Blueprint for Excellence.

该提案解决了建立临床前微超声成像核心的需求。的 FujiFilm-VisualSonics Inc. Vevo 3100高分辨率微超声系统提供最先进的成像 这将加速对退伍军人事务部医学使命重要领域的研究工作，包括精神健康 在一些实施方案中，所述药物组合物包括但不限于神经系统疾病、创伤性脑损伤、脊髓损伤、心脏病、矫形损伤和中风。此外，委员会认为， 临床前微超声成像核心将补充现有的核心（小动物集成 行为和生理评估核心，VA动物研究手术室，VA细胞和 分子评价核心，以及组织学和细胞成像核心）在研究服务在拉尔夫 H.位于南卡罗来纳州查尔斯顿的约翰逊退伍军人医疗中心。我们的目标是为我们的 研究人员，以促进从亚细胞水平到 整个动物。这些核心设施，围绕关键设备项目建设，往往比一个单一的成本 实验室可以提供，已经建立的设计，以支持和推进我们的广泛 研究计划，直接影响我们的退伍军人的医疗保健。提出的中心假设是 这一核心是能够实时评估疾病或损伤存在的临床相关技术 和进展，将提高我们的VA调查人员正在做的工作的翻译意义。这 将通过4个主要成像目标来解决假设：1）使用 标准超声技术与生理监测（2D，B模式，M模式， 心脏/呼吸门控）; 2）解决组织特异性特征（如心肌或 血管壁应力和应变（VevoTrace、VevoStrain、VevoVasc、Speckle跟踪）; 3）使用新的 增强组织成像、血流或靶向分析物定位的造影剂（ 靶向/非靶向微泡造影）;以及4）在3D上对靶组织的高级体积成像， 时间（4D成像）和整合彩色多普勒、能量多普勒和造影成像数据的能力。的 Vevo 3100高分辨率显微超声成像系统，包括先进的软件包， 小动物生理学的详细定量评估（使用脉搏波的血流测量， 彩色多普勒、心脏和血管性能和几何形状）使用多种模态（2D、3D和4D成像， MicroMarker造影剂），并可应用于常规检查的遗传、疾病和损伤模型。 我们的调查人员使用的。这些评估直接适用于代表那些 影响我们的退伍军人，如血管疾病，心脏病，神经系统疾病，肌肉骨骼 病理学、中风、创伤性脑损伤和脊髓损伤。鉴于其多功能性和集成性， 我们的核心设施，VA调查员将能够进行高分辨率的动物微超声成像， 这些数据可以与其他核心数据协同使用，例如从生理和 行为评估核心建议的核心与我们现有的核心的集成，使我们的研究人员 进行全面的生理评估这是一个独特的基础设施， 推进我们当地VAMC正在进行的研究。所要求的仪器将进一步加强 当地VA研究能力符合部门内概述的目标， 退伍军人事务部卓越蓝图。