Genomics

基因组核心实验室(GCL)是一家提供全方位服务的微阵列分析和测序设施。《核心》 通过提供DNA和RNA分析服务，支持基础、转译和临床研究 实验设计和样品准备，以进行基本数据分析和培训。样品制备 服务包括从标准来源和具有挑战性的来源提取RNA和DNA，以及样本质量 控制评估。可用的功能基因组学应用包括基因表达、阵列比较 基因组杂交、microRNA、SNP、染色质免疫沉淀和甲基化分析 用Affymetrix、llLumina或Agilent微阵列测量。通过实时荧光聚合酶链式反应验证微阵列也是 如果是这样的话。2006年，GCL扩大了其测量技术组合，将Sanger包括在内 测序以及几个下一代测序平台，来自Lon Torrent的PGM，两个 来自LLUMINA的HiSeq2000和MiSeq，来自罗氏的454FLX和454 Junior，以及来自Life的两个SOLiD5500 技术。这些不同测序技术的可用性使高通量分析成为可能 一系列基因组学应用，如变异检测、表观基因组学、转录组测序和 更多。GCL的工作人员还提供基本的生物信息学分析、培训和支持。范围广泛的 在过去的一年里，基因组核心提供的服务支持了129名研究人员的研究。 在过去的赠款期间，核心的工作为来自9个国家的484份研究人员的出版物做出了贡献 研究项目。基因组学核心被选为癌症基因组特征之一 832例多形性胶质母细胞瘤、1179例卵巢浆液性病变的中心和生成的Agilent CGH数据 囊腺癌244例，肺腺癌244例。MSKCC的表现和贡献 TCGA团队(临床医生、科学家、计算生物学家和基因学家)在试验阶段提供了帮助 该研究所在2009年开始的实际TCGA项目中获得了一席之地，作为基因组数据分析 中间。

The Genomics Core Lab (GCL) is a full-service microarray analysis and sequencing facility. The Core supports basic, translational and clinical research by providing DNA and RNA analysis services from experimental design and sample preparation to basic data analysis and training. Sample preparation services include RNA and DNA extraction from standard and challenging sources as well as sample quality control assessment. The functional genomics applications available are gene expression, array Comparative Genomic Hybridization (aCGH), microRNA, SNP, Chromatin Immunoprecipitation and methylation analysis measured with Affymetrix, lllumina or Agilent microarrays. Microarray validation by Real-Time PCR is also provided. In 2006, the GCL expanded its portfolio of measurement technologies to include Sanger sequencing as well as several Next Generation Sequencing platforms, the PGM from lon Torrent, two HiSeq2000 and a MiSeq from lllumina, a 454FLX and a 454Junior from Roche and two SOLiD5500 from Life Technologies. The availability of these different sequencing technologies enables high-throughput analysis Of a range of genomics applications, such as variant detection, epigenomics, transcriptome sequencing and more. GCL staff also provides basic bioinformatics analysis, training and support. The broad range of services provided by the Genomics Core has supported the research of 129 Investigators in/the past year. During the past grant period the work of the Core has contributed to 484 publications of researchers from 9 research programs. The Genomics Core was chosen as one of the Cancer Genome Characterization Centers and generated Agilent CGH data for 832 glioblastoma multiforme, 1179 ovarian serous cystadenocarcinomas and 244 lung adenocarcinomas. The performance and contribution of the MSKCC TCGA team (clinicians, scientists, computational biologists and genomicists) during the pilot phase helped the Institution obtain a spot in the actual TCGA project that started in 2009, as a Genome Data Analysis center.