Exploratory models of cortical consolidation

摘要 眼优势可塑性是单眼诱发的体内突触可塑性的一种典型形式 剥夺(MD)。它为视觉回路在早期生命中如何发展和重塑提供了至关重要的见解。我们 研究表明，睡眠可以增强猫的睡眠能力。我们的目标是更全面地识别潜在的 机制和它们发生时的大脑状态。为了实现这一目标，我们将开发一种新的鼠标- 基于睡眠依赖的ODP型动物模型。小鼠的MD引发生理和形态上的变化 皮质环路与食肉动物物种中描述的类似。然而，与食肉动物物种不同，分子 用于可视化神经元活动和测量信使核糖核酸的技术在小鼠中得到广泛应用。确实有 目前还没有研究利用这一模型系统来确定经验和睡眠如何影响 ODP值。在这个探索性的提案中，我们将使用多导睡眠图和钙离子的新组合- 基于荧光的在体显微镜记录睡眠和睡眠期间视觉皮质神经元的可塑性变化 保持清醒。我们还将使用翻译核糖体亲和纯化(TRAP)技术与 微阵列和RNAseq分离发生在睡眠中的转录组的变化，重塑视觉 大脑皮层。这些调查的结果将为更大范围的调查提供基础 经验和睡眠塑造了发育中的大脑。

Summary Ocular dominance plasticity (ODP) is a canonical form of synaptic plasticity in vivo triggered by monocular deprivation (MD). It has provided crucial insights into how visual circuits develop and remodel in early life. We have shown that ODP in cats is enhanced by sleep. Our goal is to more completely identify the underlying mechanisms and the brain states in which they occur. To achieve this goal we will develop a new mouse- based model of sleep-dependent ODP. MD in mice triggers physiological and morphological changes in cortical circuits similar to those described in carnivore species. However, unlike carnivore species, molecular techniques used to visualize neuronal activity and measure mRNA are widely used in mice. There are currently no studies that have exploited this model system to identify how experience and sleep influence ODP. In this exploratory proposal, we will use a novel combination of polysomnography and calcium- fluorescence based microscopy in vivo to record plastic changes in visual cortical neurons across sleep and wakefulness. We will also use Translating Ribosome Affinity Purification (TRAP) technology combined with microarray and RNAseq to isolate changes in the transcriptome that occur in the sleeping, remodeling visual cortex. The results of these investigations will provide the foundation for a larger investigation of how experience and sleep shape the developing brain.