PROTEOMICS AND COMPARATIVE GENOMIC INVESTIGATIONS REVEAL HETEROGENEITY IN EVOLUT

蛋白质组学和比较基因组研究揭示了进化的异质性

• 批准号: 7957851
• 负责人: WILLIE J SWANSON
• 金额: $ 0.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957851
• 关键词: Affect; Anterior; Biology; Bulbourethral gland structure; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Data; Data Set; Diverticulum; Employee Strikes; Environment; Evolution; Female; Funding; Fungal Genome; Genes; Genome; Genomics; Grant; Heterogeneity; Histocompatibility Testing; Human; Institution; Investigation; Mammals; Maps; Mediating; Mus; Ontology; Physiological; Prostate; Proteins; Proteomics; Research; Research Personnel; Resources; Seminal; Seminal Vesicles; Seminal fluid; Source; Tissues; United States National Institutes of Health; cell motility; comparative; experience; fitness; male; reproductive; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Male reproductive fitness is strongly affected by seminal fluid. In addition to interacting with the female environment, seminal fluid mediates important physiological characteristics of sperm, including capacitation and motility. In mammals, the male reproductive tract shows a striking degree of compartmentalization, with at least six distinct tissue types contributing material that is combined with sperm in an ejaculate. While studies of whole ejaculates have been undertaken in some species, we lack a comprehensive picture of the specific proteins produced by different accessory tissues. Here we perform proteomic investigations of six regions of the male reproductive tract in mice - seminal vesicles, anterior prostate, dorsolateral prostate, ventral prostate, bulbourethral gland, and bulbourethral diverticulum. We identify 766 proteins that could be mapped to 506 unique genes and compare them to a high-quality human seminal fluid dataset. We find that Gene Ontology functions of seminal proteins are largely conserved between mice and humans. By placing these data in an evolutionary framework, we show that seminal vesicle proteins have experienced a significantly higher rate of nonsynonymous substitution compared to the genome, which could be the result of adaptive evolution. In contrast, proteins from the other five tissues showed significantly lower nonsynonymous substitution, revealing a previously unappreciated level of evolutionary constraint acting on the majority of male reproductive proteins.

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