Genomics Core

基因组学核心

• 批准号: 9115209
• 负责人: Ralph V. Shohet
• 金额: $ 18.3万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9115209
• 关键词: Animals; Applications Grants; Area; Binding; Bioinformatics; Biological; Cardiac; Cardiovascular system; Centers of Research Excellence; Chad; Collaborations; Computer software; Consultations; Core Facility; DNA Sequence; Data; Data Analyses; Data Quality; Devices; Diagnosis; Disease; Education; Educational process of instructing; Epitopes; Equipment; Evaluation; Fostering; Funding; Genomics; Goals; Growth; Hawaii; Hawaiian population; Hospitals; Human Resources; Indium; Individual; Informatics; Institutes; Institution; Instruction; Island; Journals; Knowledge; Ligands; Molecular; NCI Center for Cancer Research; Peptides; Performance; Process; Proteins; Publications; Recovery; Recruitment Activity; Research; Research Personnel; Resources; Running; SNP genotyping; Sampling; Schools; Services; Silicon; Site; Strategic Planning; Technology; Testing; Time; Training; Transistors; Translations; Troponin; Universities; Virginia; Work; base; cost; innovation; medical schools; melting; prediction algorithm; professor; research study; skills; trend

The JABSOM Genomics Core Facility offers genomic services, experimental advice, and data analysis to researchers of the Hawaiian Islands. To date we have processed samples from over 66 individual users from multiple sites including Chaminade University, UH Manoa, UH Hilo, JABSOM, Queens and Leahi Hospitals, and the Cancer Research Center. Total invoices, in both number and dollar amount, have steadily increased on a yearly basis since the creation of the Core in March of 2009. Our major services of qPCR, Sequencing, and Microarray Hybridization are each trending steadily upwards each year. A careful evaluation of costs and recharge recovery suggests that with continued moderate growth of the activity in the Core we can achieve break-even fiscal performance after 5 years. The Core is run by a talented Manager, Steffen Oeser, who worked for 8 years at lllumina in genomic technologies. In addition to genomic services the Core provides education to users and maintains the skills of its staff. In order to enhance the offerings of the Core in this cycle of COBRE funding we have established a collaboration with the Virginia Bioinformatics Institute. The provision of sophisticated informatic expertise should increase the value of the Core offerings, especially microarray data. We also have a component of innovation in the Core, provided largely by projects of a new Assistant Professor, and COBRE young investigator, Chad Walton. These include a bioinformatic approach to ligand selection, a potential improvement in SNP identification by a gradient "melt-off", and work on a field-effect transistor that will allow us to test the capability of the ligand-prediction algorithm to generate a peptide that will bind to troponin for diagnosis of cardiac damage. Transitional COBRE support will firmly establish this core as a sustainable resource for crucial genomic services that are necessary for a molecular understanding of disease.

JABSOM基因组学核心设施为夏威夷群岛的研究人员提供基因组服务、实验建议和数据分析。到目前为止，我们已经处理了来自多个网站的66名个人用户的样本，包括Chaminade大学、UH Manoa、UH Hilo、JABSOM、皇后区和Leahi医院以及癌症研究中心。自2009年3月建立核心系统以来，发票总额无论在数量上还是在金额上都稳步增长。我们的主要服务包括定量聚合酶链式反应、测序和微阵列杂交，每年都在稳步上升。对成本和充值回收的仔细评估表明，随着核心业务活动的持续适度增长，我们可以在5年后实现盈亏平衡的财务业绩。Core由一位才华横溢的经理Steffen Oeser经营，他在llLumina基因组技术领域工作了8年。除了基因组服务外，核心还向用户提供教育，并保持其工作人员的技能。为了在本轮Cobre资助周期中加强Core的服务，我们与弗吉尼亚生物信息学研究所建立了合作关系。提供复杂的信息学专业知识应该会增加核心产品的价值，特别是微阵列数据。我们也有一个核心创新的组成部分，主要由一位新的助理教授和科布雷年轻的研究员查德·沃尔顿的项目提供。这些包括生物信息学的配基选择方法，通过梯度“熔融”来识别SNP的潜在改进，以及场效应晶体管的工作，这将使我们能够测试配基预测算法产生与肌钙蛋白结合的肽的能力，以诊断心脏损伤。过渡科布雷公司的支持将坚定地将这一核心确立为关键基因组服务的可持续资源，这些服务是了解疾病的分子所必需的。