RODENT PHENOTYPING CORE & OTHER TECHNICAL CORES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在过去的15年里，已经开发出了一些方法，可以在小鼠身上进行与在更大动物中进行的相同范围的心血管评估。这些方法现在包括血压测量(有创性、植入性和非侵入性)、超声心动图、心电图术和有创血流动力学。活动测量包括新陈代谢、体力活动和运动耐量的测定。随着小鼠的外科手术已经小型化，研究人员增加了一系列心血管压力，包括主动脉环扎术以增加血流动力学负荷，各种造成心肌梗死或缺血的方法，以及通过渗透性微泵输注强心剂。其中许多技术需要高技能的操作员，研究人员不容易掌握，因为他们通常比生理学和显微外科更了解分子生物学或生物化学。老鼠表型核心的设计是为了将这些技术带给科布雷的研究人员，在大学社区的任何地方，他们都将推动心血管研究。我们的具体目标源于这一优先事项。第一个目标是为科布雷的研究人员提供全面的小鼠表型和操作。第二是向普通大学的研究人员提供同样的服务，以促进和鼓励将其他领域的新生物学见解应用于心血管疾病问题。第三是开发评估和研究小鼠心脏和血管系统的新方法和能力。我们计划在核心部分提供广泛的基本心血管评估曲目。我们还将修改一种用于在小鼠身上使用的生物活性物质的组织特异性定位的新技术。我们的目标是提高Cobre研究的效率和成功，并鼓励应用小鼠模型，帮助将基础研究带入生理学领域，更接近临床应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Over the past 15 years methods have been developed that allow the same range of cardiovascular assessments in mice that can be performed in larger animals. These methods now include blood pressure measurements, (invasive, implanted and non-invasive), echocardiography, electrocardiography, and invasive hemodynamics. Activity measurements include determinations of metabolism, physical activity, and exercise tolerance. As surgical procedures have been miniaturized for the mouse, investigators have added a range of cardiovascular stresses including aortic banding to increase hemodynamic load, various approaches to produce myocardial infarction or ischemia, and infusion of cardiotonic agents by osmotic minipumps. Many of these techniques require highly skilled operators and are not easily mastered by investigators who are usually more knowledgeable about molecular biology or biochemistry than physiology and microsurgery. The Mouse Phenotyping Core is designed to bring these techniques to investigators in the COBRE and wherever in the University community they will advance cardiovascular investigation. Our specific aims derive from this priority. The first aim is to provide comprehensive mouse phenotyping and manipulation to the investigators of the COBRE. The second is to provide these same services to general University researchers to facilitate and encourage application of new biological insights in other fields to problems of cardiovascular disease. The third is to develop new methods and capabilities for assessing and investigating the mouse heart and vasculature. We plan to offer a wide repertoire of basic cardiovascular assessments in the core. We will also modify a novel technology for tissue-specific localization of bioactive agents for use in mice. Our goals are to enhance the efficiency and thus success of COBRE research, and to encourage the application of mouse models to help bring basic research into the realm of physiology and closer to clinical application.