RODENT PHENOTYPING CORE & OTHER TECHNICAL CORES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 在过去的15年里，已经开发出了一些方法，可以在小鼠中进行与在大型动物中进行的相同范围的心血管评估。这些方法现在包括血压测量（侵入性、植入式和非侵入性）、超声心动图、心电图和侵入性血流动力学。活动测量包括新陈代谢、体力活动和运动耐量的测定。由于外科手术已经小型化的小鼠，研究人员已经增加了一系列的心血管应力，包括主动脉束带，以增加血液动力学负荷，各种方法来产生心肌梗死或缺血，并通过渗透微型泵输注强心剂。这些技术中的许多需要高度熟练的操作者，并且不容易被通常比生理学和显微外科学更了解分子生物学或生物化学的研究人员掌握。小鼠表型分析核心旨在将这些技术带给COBRE的研究人员，以及大学社区的任何地方，他们将推进心血管研究。我们的具体目标源自这一优先事项。第一个目标是为COBRE的研究人员提供全面的小鼠表型和操作。第二是为一般大学研究人员提供这些相同的服务，以促进和鼓励将其他领域的新生物学见解应用于心血管疾病问题。第三是开发新的方法和能力，用于评估和研究小鼠心脏和血管系统。我们计划在核心中提供广泛的基本心血管评估。我们还将修改一种新的技术，用于组织特异性定位的生物活性剂在小鼠中使用。我们的目标是提高COBRE研究的效率，从而取得成功，并鼓励小鼠模型的应用，以帮助将基础研究带入生理学领域并更接近临床应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Over the past 15 years methods have been developed that allow the same range of cardiovascular assessments in mice that can be performed in larger animals. These methods now include blood pressure measurements, (invasive, implanted and non-invasive), echocardiography, electrocardiography, and invasive hemodynamics. Activity measurements include determinations of metabolism, physical activity, and exercise tolerance. As surgical procedures have been miniaturized for the mouse, investigators have added a range of cardiovascular stresses including aortic banding to increase hemodynamic load, various approaches to produce myocardial infarction or ischemia, and infusion of cardiotonic agents by osmotic minipumps. Many of these techniques require highly skilled operators and are not easily mastered by investigators who are usually more knowledgeable about molecular biology or biochemistry than physiology and microsurgery. The Mouse Phenotyping Core is designed to bring these techniques to investigators in the COBRE and wherever in the University community they will advance cardiovascular investigation. Our specific aims derive from this priority. The first aim is to provide comprehensive mouse phenotyping and manipulation to the investigators of the COBRE. The second is to provide these same services to general University researchers to facilitate and encourage application of new biological insights in other fields to problems of cardiovascular disease. The third is to develop new methods and capabilities for assessing and investigating the mouse heart and vasculature. We plan to offer a wide repertoire of basic cardiovascular assessments in the core. We will also modify a novel technology for tissue-specific localization of bioactive agents for use in mice. Our goals are to enhance the efficiency and thus success of COBRE research, and to encourage the application of mouse models to help bring basic research into the realm of physiology and closer to clinical application.