Mechanism of Candida albicans rupture on biomimetic NSS

项目摘要/摘要 项目名称：白假丝酵母菌破裂机理研究 PI：Dennis LaJeunesse(UNG/纳米科学) 这项研究的目标是描述纳米尺度的机械相互作用 在致病酵母细胞、白色念珠菌和破裂细胞的纳米结构之间 以及确定确定和控制NSS诱导的酵母的条件 细胞破裂。我们的长远目标包括应用新高中计划作为一种机械手段。 控制病原真菌生长和生物被膜形成。在这项拟议的研究中 我们有三个目标：1)表征白色念珠菌与破裂的NSS的相互作用 确定细胞形态和黏附在NSS诱导的细胞中所起的作用 破裂；2)在NSS破裂过程中对酵母细胞进行结构和化学分析 以确定白念珠菌细胞的哪些方面容易受到NSS的攻击；以及3) 确定耐药株白念珠菌如何应对国家安全局的攻击。这个 拟议中的研究将是对人类相互作用的第一次系统研究 病原真菌白色念珠菌和NSS。单独或联合使用抗菌NSS 与传统抗真菌药物相比，对新型抗菌药物有着巨大的潜在影响 治疗。这项工作将使我们更深入地了解 NSS诱导的反应和将促进NSS应用的实用知识 真菌控制。除了拟议研究的重要性外，这项工作还将 对北卡罗来纳州中部的皮德蒙特社区也做出了重大贡献。《关节》 纳米科学和纳米工程学院的使命是培养学生，吸引 行业，并参与北卡罗来纳州中部皮埃蒙特的社区。这项工作 将涉及博士生和几名本科生研究人员的培训 基本STEM计划，并将成为社区外展的主要组成部分 JSNN科学节和Nanobus等计划。诺斯大学 格林斯伯勒分校的卡罗莱纳是一家少数族裔服务机构，因此这项研究也将 旨在向服务不足的人群推广STEM教育和培训。

Project Summary/Abstract Project Title: Mechanism of Candida albicans rupture on biomimetic NSS PI: Dennis LaJeunesse (UNCG/Nanoscience) The goals of this research are to characterize the nanoscale mechanical interactions between a pathogenic yeast cell, Candida albicans and a cell-rupturing nanostructured surface (NSS) and to define the conditions that determine and control NSS-induced yeast cell rupture. Our long term goals involve the application of NSS’s as a mechanical means of controlling pathogenic fungal growth and biofilm formation. In this proposed research we have three aims: 1) characterize the interactions of C. albicans with a rupturing NSS and determine the roles that cell morphology and adhesion play in NSS induced cell rupture; 2) perform a structural and chemical analysis of the yeast cell during NSS rupture to determine what aspects of the C. albicans cell are vulnerable to NSS assault; and 3) determine how drug resistance strains of C. albicans respond to NSS assault. The proposed research will be the first systematic study of the interactions of a human pathogenic fungi C. albicans and a NSS. Antimicrobial NSS used alone or in conjunction with traditional antifungal drugs have tremendous potential impact for novel antimicrobial treatments. This work will provide greater insight into the fundamental mechanisms of NSS-induced response and practical knowledge that will facilitate the application of NSS fungal control. In addition to the importance of the proposed research, this work will also contribute significantly the central North Carolina Piedmont community as well. The Joint School of Nanoscience and Nanoengineering has a mission to train students, attract industry, and engage the community of the central North Carolina Piedmont. The work will involve the training of doctoral students and several undergraduate researchers in essential STEM programs and will be a major component of community outreach programs such as the JSNN science festival and Nanobus. The University of North Carolina at Greensboro is a minority serving institution and thus this research will also serve to promote STEM education and training to an underserved population.