Analytical and Morphological Core

分析和形态核心

• 批准号: 9212653
• 负责人: XIAO-PING YANG
• 金额: $ 34.86万
• 依托单位: HENRY FORD HEALTH SYSTEM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2019-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9212653
• 关键词: Adenosine; Analytical Chemistry; Angiotensins; Apoptosis; Biological Assay; Blinded; Blood capillaries; Body Fluids; Cardiac Myocytes; Cell Adhesion Molecules; Cells; Chemicals; Collagen; Creatinine; Cultured Cells; Cyclic AMP; Data; Electrophoretic Mobility Shift Assay; Ensure; Enzyme-Linked Immunosorbent Assay; Enzymes; Equipment; Gene Expression; Guanosine Monophosphate; Heart; Histologic; Human Resources; Hydroxyproline; Hypertension; Image Analysis; Immunoassay; Individual; Infarction; Infiltration; Inflammatory; Instruction; Kidney; Kininogenase; Lesion; Link; Measurement; Measures; Morphology; Necrosis; Nitrates; Nitrites; Nuclear; Organ; Oxidative Stress; Pathogenesis; Periodic acid Schiff stain method; Periodicity; Photography; Plasma; Polymerase Chain Reaction; Productivity; Program Research Project Grants; Prostaglandins; Proteins; Radioimmunoassay; Renin; Sampling; Staining method; Stains; Time; Tissues; capillary; cost; cytokine; density; interstitial; laboratory facility; lysylproline; tissue processing

The primary purpose of the Analytical and Morphological Core (Core B) is to provide established and centralized assays and expert personnel for projects of the Program Project Grant (PPG), which includes analytical chemistry, enzyme-linked immunoassay (ELISA), radioimmunoassay (RIA), real-time polymerase chain reaction (RT-PCR), electrophoretic mobility shift assay (EMSA) and histological, morphological and imaging analysis. Analytical chemistry covers measurement of nitrites/nitrates, creatinine, hydroxyproline and kallikrein activity. ELISA will be used to measure N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), prostanoids, cytokines, unnary protein, etc. RIA will be used to measure plasma renin activity and renin and angiotensin concentrations, cyclic adenosine (cAMP) and guanosine monophosphates (cGMP). RT-PCR and EMSA will be performed to measure gene expression and nuclear factor kappaB ( N F K B ) activation and translocation. All chemical analyses and bioassays will be performed on samples obtained from tissues, body fluids or cultured cells. Histomorphology includes tissue processing, embedding, cutting, histological and immunohistochemical staining and photography for measurement of a) infarct size, cardiomyocyte size, capillary density, interstitial and perivascular collagen; 2) inflammatory cell infiltration, adhesion molecules, markers of oxidative stress, apoptosis and necrosis in the vasculature, heart and kidney; and c) periodic acid Schiff (PAS) staining for kidney lesions. The Project Leaders will analyze the histological data in a blinded fashion with the assistance of Core B. In addition, the Analytical and Morphological Core will relieve Project Leaders from performing these labor-intensive and time-consuming analyses themselves and instead allow them more time to pursue their scientific efforts. The anticipated Analytical and Morphological Core's effort for each project is estimated as Project I, 55%; Project 11, 30%, Project HI, 10%; and Project IV, 5%. RELEVANCE (See instructions): By centralizing analytical and morphological studies for Project Leaders, Core B will help integrate participating projects by providing consistent, reliable and objectively analytic and morphological assays, which will promote both quality and productivity of participating projects. Core B will ensure cost efficiency in terms of labor and laboratory facilities/equipment to avoid duplication of efforts by individual Project Leaders.

分析和形态学核心（核心B）的主要目的是提供已确定的 项目资助（PPG）项目的集中测定和专家人员，包括 分析化学，酶联免疫测定（ELISA），放射免疫测定（RIA），实时聚合酶 应用RT-PCR、电泳迁移率变动分析（EMSA）和组织学、形态学和 图像分析分析化学包括亚硝酸盐/硝酸盐、肌酐、羟脯氨酸的测量 和激肽释放酶活性。ELISA将用于测量N-乙酰基-丝氨酰-赖氨酰-脯氨酸（Ac-SDKP）， 前列腺素、细胞因子、一元蛋白等。RIA将用于测定血浆肾素活性和肾素， 血管紧张素浓度、环腺苷（cAMP）和鸟苷一磷酸（cGMP）。RT-PCR 将进行EMSA以测量基因表达和核因子κ B（NF K B）活化， 易位所有化学分析和生物测定将对从组织中获得的样本进行， 体液或培养的细胞。组织形态学包括组织处理、包埋、切割、组织学 以及免疫组织化学染色和照相术，用于测量a）梗塞大小，心肌细胞大小， 毛细血管密度，间质和血管周围胶原蛋白; 2）炎性细胞浸润，粘附分子， 血管系统、心脏和肾脏中的氧化应激、细胞凋亡和坏死的标志物;和 肾脏病变的Schiff（PAS）染色。项目负责人将在盲态下分析组织学数据。 在核心B的协助下进行时尚。此外，分析和形态核心将减轻项目 领导者不需要自己执行这些劳动密集型和耗时的分析，而是允许 让他们有更多的时间来进行科学研究。预期的分析和形态学核心的努力 每个项目的预算为：项目I，55%;项目11，30%;项目III，10%;项目IV，5%。 相关性（参见说明）： 通过为项目领导人集中分析和形态研究，核心B将有助于整合 通过提供一致、可靠和客观的分析和形态学检测，参与项目， 以提高参与计划的质素和生产力。核心B将确保 劳动力和实验室设施/设备的条款，以避免单个项目负责人的重复工作。