Analytical and Morphological Core

分析和形态核心(核心B)的主要目的是提供已建立的和 计划项目赠款(PPG)项目的集中化验和专家人员，包括 分析化学、酶联免疫分析、放射免疫分析、实时聚合酶 链式反应(RT-PCR)、凝胶迁移率改变分析(EMSA)和组织学、形态学和 影像分析。分析化学包括亚硝酸盐/硝酸盐、肌酐、羟脯氨酸的测量 和激肽释放酶的活性。测定N-乙酰-丝氨酸-天冬氨酰-赖氨酸(Ac-SDKP)， 前列腺素、细胞因子、单一蛋白等。RIA将用于检测血浆肾素活性和肾素及 血管紧张素浓度、环腺苷(CAMP)和一磷酸鸟苷(CGMP)。逆转录聚合酶链式反应 并将进行EMSA以测量基因表达和核因子kappaB(N F K B)的激活和 易位。所有的化学分析和生物分析都将对从组织中获得的样本进行， 体液或培养细胞。组织形态学包括组织加工、包埋、切割、组织学 以及免疫组织化学染色和摄影来测量a)梗死面积，心肌细胞大小， 毛细血管密度、间质及血管周围胶原；2)炎性细胞浸润、黏附分子； 血管、心脏和肾脏氧化应激、细胞凋亡和坏死的标记物；以及c)高碘酸 肾脏病变的席夫(PAS)染色。项目负责人将盲目地分析组织学数据 时尚在核心B的帮助下。此外，分析和形态核心将缓解项目 领导者自己执行这些劳动密集型和耗时的分析，而不是允许 他们有更多的时间进行科学研究。预期的分析和形态核心的努力 每个项目估计为项目I占55%；项目11占30%；项目HI占10%；项目四占5%。 相关性(请参阅说明)： 通过为项目负责人集中分析和形态研究，核心B将帮助整合 通过提供一致、可靠和客观的分析和形态分析，参与项目， 这将提高参与项目的质量和生产率。核心B将确保在 劳动力和实验室设施/设备的条款，以避免个别项目负责人的重复工作。

The primary purpose of the Analytical and Morphological Core (Core B) is to provide established and centralized assays and expert personnel for projects of the Program Project Grant (PPG), which includes analytical chemistry, enzyme-linked immunoassay (ELISA), radioimmunoassay (RIA), real-time polymerase chain reaction (RT-PCR), electrophoretic mobility shift assay (EMSA) and histological, morphological and imaging analysis. Analytical chemistry covers measurement of nitrites/nitrates, creatinine, hydroxyproline and kallikrein activity. ELISA will be used to measure N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), prostanoids, cytokines, unnary protein, etc. RIA will be used to measure plasma renin activity and renin and angiotensin concentrations, cyclic adenosine (cAMP) and guanosine monophosphates (cGMP). RT-PCR and EMSA will be performed to measure gene expression and nuclear factor kappaB ( N F K B ) activation and translocation. All chemical analyses and bioassays will be performed on samples obtained from tissues, body fluids or cultured cells. Histomorphology includes tissue processing, embedding, cutting, histological and immunohistochemical staining and photography for measurement of a) infarct size, cardiomyocyte size, capillary density, interstitial and perivascular collagen; 2) inflammatory cell infiltration, adhesion molecules, markers of oxidative stress, apoptosis and necrosis in the vasculature, heart and kidney; and c) periodic acid Schiff (PAS) staining for kidney lesions. The Project Leaders will analyze the histological data in a blinded fashion with the assistance of Core B. In addition, the Analytical and Morphological Core will relieve Project Leaders from performing these labor-intensive and time-consuming analyses themselves and instead allow them more time to pursue their scientific efforts. The anticipated Analytical and Morphological Core's effort for each project is estimated as Project I, 55%; Project 11, 30%, Project HI, 10%; and Project IV, 5%. RELEVANCE (See instructions): By centralizing analytical and morphological studies for Project Leaders, Core B will help integrate participating projects by providing consistent, reliable and objectively analytic and morphological assays, which will promote both quality and productivity of participating projects. Core B will ensure cost efficiency in terms of labor and laboratory facilities/equipment to avoid duplication of efforts by individual Project Leaders.