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Investigating Molecular Assembly and Dynamics of Tick Cement proteins

研究蜱水泥蛋白的分子组装和动力学

基本信息

批准号：
9304705
负责人：
SARAH E MORGAN
金额：
$ 44.4万
依托单位：
UNIVERSITY OF SOUTHERN MISSISSIPPI
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-20 至 2021-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9304705
关键词：
Adhesives Amblyomma Animals Anticoagulants Antifreeze Applications Grants Artificial Membranes Biochemical Biocompatible Materials Biological Biological Assay Biological Process Biomechanics Blood Cell Proliferation Cells Characteristics Climate Co-Immunoprecipitations Collaborations Collection Communicable Diseases Cone Data Dermacentor Dermal Dermis Development Electron Microscopy Environment Family GRP gene Gene Expression Genes Glycine Health Hemostatic Agents Heterogeneity Hour Human Immune response Inflammatory Response Injection of therapeutic agent Insect Proteins Interruption Knowledge Methods Microbe Monoclonal Antibodies Morphology Oral cavity Organism Pain Pathogenesis Pathogenicity Phase Physiological Processes Plants Play Positioning Attribute Post-Translational Protein Processing Prevention Process Property Protein Family Proteins Proteomics Pruritus Public Health Quantitative Reverse Transcriptase PCR RNA Interference Reagent Recombinant Proteins Recombinants Research Role Saliva Salivary Salivary Glands Secure Skin Stress Structural Protein System Techniques Temperature Testing Ticks Time Transcript Up-Regulation Vascular blood supply Vector-transmitted infectious disease antimicrobial biomaterial compatibility design experimental study fascinate feeding genetic approach immunoregulation innovation insight mechanical properties molecular assembly/self assembly molecular dynamics multidisciplinary nanomechanical nanomechanics novel pathogen prevent protein expression reverse genetics scaffold student training tool transcriptome sequencing

项目摘要

Summary Ticks must use their barbed mouthparts (hypostome) to pierce deeply into the host’s dermis (skin), then encase the hypostome in a cement cone in order to maintain a firm, pain and itch- free attachment, subsequently transmitting infectious disease agents into the victim. Two types of cement have been found in ticks; early cement which is secreted only minutes after attachment and hardens rapidly, and late cement which is secreted 24 hours after attachment to the host and hardens gradually. These secreted cement cones are composed of several glycine-rich proteins (GRPs) which are a large family of diverse proteins that are known for their adhesive and tensile characteristics. Such properties may play an important role in host skin attachment to gain an uninterrupted supply of blood over a prolonged period of time. Our long- term plan in the AREA grant application is to reveal the functions and biochemical properties of novel GRPs in cement cone assembly, stealth attachment, hematophagy (blood feeding) and climatic adaptation. We will test our central hypothesis that biochemical and biomechanical properties of GRPs assist in the undetected attachment to the host, providing access to an uninterrupted supply of blood by serving as scaffolding for the cement cone apparatus. Our multidisciplinary team will test the hypothesis using a combination of artificial membrane feeding, structural, proteomics, and reverse genetics approaches. Specific aims are to: 1) determine the role of unique salivary GRPs in cement cone assembly and secure attachment by using an artificial membrane feeding system, and 2) define the biochemical properties of uncharacterized recombinantly expressed cement cone proteins. Targeting tick cement proteins may provide an innovative way to create a hostile situation for tick attachment, and ultimately interrupt the inoculation of tick-borne pathogens into the host. Understanding the mechanisms of cement assembly that allow the tick to secure a stable, stealthy attachment will provide insight into developing new control and prevention methods.

总结