SCAPE microscopy for high-speed in-vivo volumetric microscopy in behaving organisms

SCAPE 显微镜用于行为生物体的高速体内体积显微镜

• 批准号: 9328178
• 负责人: Elizabeth M. C. Hillman
• 金额: $ 50.48万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9328178
• 关键词: 3-Dimensional; Address; Adoption; Adult; Apical; Area; Behavior; Behavioral; Behavioral Assay; Biomedical Research; Brain; Brain imaging; Caenorhabditis elegans; Cells; Collaborations; Complex; Computer software; Data; Dendrites; Development; Drosophila genus; Drosophila melanogaster; Event; Genetic; Geometry; Hybrids; Image; Imaging Techniques; Individual; Institutes; Laboratories; Larva; Laser Scanning Microscopy; Lasers; Legal patent; Light; Lighting; Microscopy; Mind; Modeling; Motion; Motor; Mus; Nature; Nervous system structure; Neurobiology; Neurons; Neurosciences Research; Noise; Optics; Organism; Pattern; Penetration; Performance; Photons; Play; Publishing; Research Personnel; Resolution; Rewards; Rodent; Role; Sampling; Scanning; Signal Transduction; Somatosensory Cortex; Speed; Structure; System; Task Performances; Techniques; Testing; Three-Dimensional Imaging; Time; Tissues; Translating; Translations; Walking; Work; Zebrafish; awake; base; behavioral response; brain behavior; cellular imaging; cost; fly; genetic manipulation; high risk; imaging approach; imaging platform; improved; in vivo; information processing; interest; lens; neural circuit; neuroimaging; novel strategies; optogenetics; photonics; prototype; public health relevance; relating to nervous system; somatosensory; spatiotemporal; tool; two-photon; user-friendly; whole body imaging

 DESCRIPTION (provided by applicant): Despite the growing availability of optical markers of neuronal activity, as well as genetic tools for optical manipulation, current optical microscopy techniques for imaging the intact brain at cellular resolution have approached their limits, particularly in terms of 3D volumetric imaging speeds. The brain and nervous system is inherently 3D, with cortical layers playing specific roles in information processing. Small organisms such as Drosophila melanogaster (fruit fly), Danio rerio (zebrafish) and Caenorhabditis elegans, have become valuable platforms for neuroscience research and genetic manipulation, and offer the chance to capture the entire nervous system of a complete, behaving organism. However, for both rodent brain and small organism microscopy, current techniques are limited to slow volumetric imaging rates, or single-plane acquisition. We recently developed a transformative new approach to high speed 3D microscopy called Swept, Confocally-Aligned Planar Excitation (SCAPE) microscopy. SCAPE was conceived as a way to dramatically improve volumetric imaging speeds, while maintaining a simple optical layout and image acquisition geometry. SCAPE is a hybrid between light-sheet microscopy and laser scanning confocal which overcomes the major speed barriers of both techniques. Recently published in Nature Photonics, SCAPE can image at volume rates 10-100 x faster than laser scanning microscopy or fast light-sheet imaging. We have demonstrated imaging of cellular-level structure and function in both the awake, behaving rodent brain and freely moving Drosophila melanogaster larvae at 10-20 volumes per second (VPS) over large fields of view. A further feature of SCAPE is its simple, single, stationary objective, permitting 3D imaging with no motion at the sample, making it well suited for integration with pattered optogenetic manipulation of cells during high-speed 3D imaging. Having achieved `proof of concept' we now wish to develop SCAPE into a tool for routine use by neuroscientists working in both small organisms, for in-toto imaging of cellular activity and behavior, and in awake, behaving mouse brain. The former will be achieved through development and translation of an improved beta prototypes `1P-SCAPE' system, with development of user friendly acquisition software, data handling and analysis platforms, and ultimately its deployment and support for use in studies of somatosensory integration in adult and larval Drosophila. For mouse brain imaging, we propose to test the limits of SCAPE by exploring two- photon implementation (2P-SCAPE), which will afford deeper penetration imaging into scattering tissues such as the rodent brain.



项目成果

Neuromuscular basis of Drosophila larval rolling escape behavior.

果蝇幼虫滚动逃生行为的神经肌肉基础。

• DOI: 10.1073/pnas.2303641120
• 发表时间: 2023-12-19
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Cooney, Patricia C.;Huang, Yuhan;Li, Wenze;Perera, Dulanjana M.;Hormigo, Richard;Tabachnik, Tanya;Godage, Isuru S.;Hillman, Elizabeth M. C.;Grueber, Wesley B.;Zarin, Aref A.
• 通讯作者: Zarin, Aref A.

The mesencephalic locomotor region recruits V2a reticulospinal neurons to drive forward locomotion in larval zebrafish.

• DOI: 10.1038/s41593-023-01418-0
• 发表时间: 2023-10
• 期刊: NATURE NEUROSCIENCE
• 影响因子: 25
• 作者: Carbo-Tano, Martin;Lapoix, Mathilde;Jia, Xinyu;Thouvenin, Olivier;Pascucci, Marco;Auclair, Francois;Quan, Feng B.;Albadri, Shahad;Aguda, Vernie;Farouj, Younes;Hillman, Elizabeth M. C.;Portugues, Ruben;Del Bene, Filippo;Thiele, Tod R.;Dubuc, Rejean;Wyart, Claire
• 通讯作者: Wyart, Claire

The spatial and temporal structure of neural activity across the fly brain.

• DOI: 10.1038/s41467-023-41261-2
• 发表时间: 2023-09-11
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Schaffer ES;Mishra N;Whiteway MR;Li W;Vancura MB;Freedman J;Patel KB;Voleti V;Paninski L;Hillman EMC;Abbott LF;Axel R
• 通讯作者: Axel R

Whole-Volume Clustering of Time Series Data from Zebrafish Brain Calcium Images via Mixture Modeling.

通过混合建模对斑马鱼脑钙图像的时间序列数据进行全体积聚类。

• DOI: 10.1002/sam.11366
• 发表时间: 2018
• 期刊: Statistical analysis and data mining
• 影响因子: 1.3
• 作者: Nguyen,HienD;Ullmann,JeremyFP;McLachlan,GeoffreyJ;Voleti,Venkatakaushik;Li,Wenze;Hillman,ElizabethMC;Reutens,DavidC;Janke,AndrewL
• 通讯作者: Janke,AndrewL