Multi-Photon endomicroscope for real-time in vivo vertical sectioning

用于实时体内垂直切片的多光子内窥镜

基本信息

  • 批准号:
    9261386
  • 负责人:
  • 金额:
    $ 33.35万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-07-18 至 2019-04-30
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): The goal of this Bioengineering Research Grant (BRG) is to produce a novel multi-photon microendoscopy instrument that can rapidly vary focal depth and thus produce real-time vertical cross-sectional images from biological tissue in vivo, and to validate instrument performance in imaging of targeted peptide markers for colon cancer. Relative to prior miniature multi-photon instruments, the proposed instrument will feature much faster axial scanning for cross-sectional imaging without motion artifacts (frame rate 5-10 Hz) while maintaining a small diameter (3.4 mm) for compatibility with small animal imaging in the gastrointestinal tract. Instrument performance is expected to meet goals of sub-cellular resolution and deep tissue penetration with substantial field of view. Instrument development will be based on a multi-disciplinary collaboration of medical and engineering expertise in actuation, instrument design, optics, and cancer biology. Axial (into-tissue) scanning capabilities will be provided by high-speed, large- stroke thin-film PZT vertical translational actuators. A fiber-coupled, remote scanning architecture will permit modular insertion of optimized optics and actuators in a small handheld instrument. Instrument verification will be performed through benchmarking against of resolution, penetration depth, frame rate, and signal-to-noise ratio on standardized targets and/or phantom tissues. Full instrument validation will be performed in studies of in vivo imaging of genetically-engineered mouse models of cancer. Mice will be labeled with both targeted, dye-labeled peptides and general tissue dyes. Peptides will be optimized for bonding to specific targets exhibited in human disease and the mouse model. The optimized peptide will be characterized for binding affinity. In vivo imaging of the peptide with different fluorescent reporters will be used to identify optimal operating parameters for deep multi-photon imaging and characterize instrument performance.


项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)

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Kenn R Oldham其他文献

Kenn R Oldham的其他文献

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{{ truncateString('Kenn R Oldham', 18)}}的其他基金

Implantable 3D fluorescence imaging with high-speed, addressable laser scanning in moving mice
通过高速、可寻址激光扫描对移动小鼠进行植入式 3D 荧光成像
  • 批准号:
    10614795
  • 财政年份:
    2023
  • 资助金额:
    $ 33.35万
  • 项目类别:
Microsystems imaging system for epithelial-derived cancer heterogeneity
用于上皮源性癌症异质性的微系统成像系统
  • 批准号:
    10428365
  • 财政年份:
    2019
  • 资助金额:
    $ 33.35万
  • 项目类别:
Microsystems imaging system for epithelial-derived cancer heterogeneity
用于上皮源性癌症异质性的微系统成像系统
  • 批准号:
    10647732
  • 财政年份:
    2019
  • 资助金额:
    $ 33.35万
  • 项目类别:
Microsystems imaging system for epithelial-derived cancer heterogeneity
用于上皮源性癌症异质性的微系统成像系统
  • 批准号:
    10237228
  • 财政年份:
    2019
  • 资助金额:
    $ 33.35万
  • 项目类别:
Microsystems imaging system for epithelial-derived cancer heterogeneity
用于上皮源性癌症异质性的微系统成像系统
  • 批准号:
    10000913
  • 财政年份:
    2019
  • 资助金额:
    $ 33.35万
  • 项目类别:

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