Flow Cytometry & Cell Sorter Core

流式细胞术

• 批准号: 9272411
• 负责人: LingLin Xie
• 金额: $ 19.08万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9272411
• 关键词: Area; Cell Culture Techniques; Cell Separation; Cells; Core Facility; Development; Distant; Educational process of instructing; Environment; Fellowship; Flow Cytometry; Funding; Health; Health Professional; Home environment; Laboratories; Learning; Medical Students; North Dakota; Population; Research; Research Infrastructure; Research Personnel; Residencies; Services; base; career; experience; graduate student; research and development; stem cell technology; undergraduate student

The development of the core facilities has been a learning experience for the PI of the NO INBRE. The major lesson learned since initial funding of the NO INBRE is that in a low population, large land mass state with a limited investigator base, one must develop core facilities that provide services that cannot be purchased through commercial entities. This certainly applies to flow and cell sorting since there is no other facility capable of sorting cells in the entire state of North Dakota. Likewise, it is very difficult, if not impossible, to perform flow through commercial entities or at academic centers distant from one's home laboratory. This is especially true for the sorting of cells, where in most instances immediate processing and/or culture of the product is necessary. A prime example of this situation is in the cutting-edge technology of stem cells, where research in this area is difficult, if not impossible, without the ability to sort and capture the resulting cells for culture and/or analysis. The NO INBRE has identified this core as essential for the further development of research, teaching and service in NO. Simply stated, at some juncture in their undergraduate career, every undergraduate student destined to enter the graduate student or health professional pipeline should have been exposed to flow and cell sorting. The same is true for graduate students destined for postdoctoral fellowships and medical students progressing to residencies.

核心设施的开发对于 NO INBRE 的 PI 来说是一次学习经历。自 NO INBRE 首次资助以来吸取的主要教训是，在人口少、土地面积大、调查人员基础有限的州，必须开发提供无法通过商业实体购买的服务的核心设施。这当然适用于流式细胞分选，因为整个北达科他州没有其他设施能够对细胞进行分选。同样，通过商业实体或远离家庭实验室的学术中心进行流程即使不是不可能，也是非常困难的。对于细胞分选尤其如此，在大多数情况下，需要立即处理和/或培养产品。这种情况的一个典型例子是干细胞的尖端技术，如果没有能力分选和捕获所得细胞进行培养和/或分析，该领域的研究即使不是不可能，也是很困难的。 NO INBRE 认为这一核心对于 NO 的研究、教学和服务的进一步发展至关重要。简而言之，在本科生职业生涯的某个阶段，每个注定要进入研究生或健康专业管道的本科生都应该接触过流式细胞分选和细胞分选。对于打算获得博士后奖学金的研究生和晋升为住院医师的医学生来说也是如此。