CSHL Advanced Techniques In Molecular Neuroscience Course

CSHL 分子神经科学高级技术课程

• 批准号: 9248443
• 负责人: DAVID J. STEWART
• 金额: $ 10.57万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-06 至 2020-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9248443
• 关键词: Affinity Chromatography; Animals; Applied Research; Area; Attention; Basic Science; Biological Assay; Chromatin; Chromatin Structure; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Course Content; Disease; Electrophysiology (science); Electroporation; Ensure; Environment; Exercise; Faculty; Funding; Future; Gene Targeting; Gene Transfer; Genes; Home environment; Image; Individual; Infection; Institution; Laboratories; Mainstreaming; Mammalian Cell; Mental Health; Messenger RNA; Methodology; Methods; Molecular; Molecular Neurobiology; Neurobiology; Neuroglia; Neurons; Neurosciences; Neurosciences Research; New York; Participant; Pharmacology; Physicians; Postdoctoral Fellow; Process; Publications; RNA Interference; Reporting; Research; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Ribosomes; Role; Scientist; Series; Specific qualifier value; Students; Techniques; Time; Training; Training Programs; Transfection; Transgenic Organisms; Translating; United States; Update; Virus; base; design; experience; forging; gene delivery system; genome editing; graduate student; in vivo; instructor; interest; laboratory curriculum; laboratory manuals; lecturer; medical specialties; member; mid-career faculty; nervous system disorder; online resource; programs; public health relevance; relating to nervous system; vector; work-study

 DESCRIPTION (provided by applicant): CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2015 - 2019, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive 2.5 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include an introduction to the design and use of animal virus vectors in neuroscience research; the use of CRISPR genome editing and RNAi approaches for regulating the expression of specific genes in neurons and glia; practical exercises in gene delivery systems including mammalian cell infection and transfection and electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; real time RT-PCR analyses; assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by translating ribosome affinity purification. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.

 描述（由申请人提供）：CSHL实验室课程在分子神经科学的先进技术拟议的冷泉港实验室（CSHL）课程先进技术在分子神经科学（ATMN），将于2015年7月至2019年每年举行，是CSHL的神经生物学研究生培训计划的一部分。ATMN是一个简短，密集的2.5周动手课程，准备学生直接进入研究，利用先进和/或专业的分子技术在神经生物学研究的当代方面。课程中的实验模块将包括介绍神经科学研究中动物病毒载体的设计和使用;使用CRISPR基因组编辑和RNAi方法调节神经元和神经胶质细胞中特定基因的表达;基因递送系统的实际练习，包括哺乳动物细胞感染和转染以及体内靶向基因转移的电穿孔技术;介绍BAC转基因载体的总体策略、使用和设计;真实的时间RT-PCR分析;神经元中染色质和染色质结构的测定，以及通过翻译核糖体亲和纯化从特定神经亚型分离mRNA。实验技术是在它们可能提供的更广泛的概念进步的背景下教授的。该课程邀请在各自领域做出重大贡献的讲师就当前研究发表最新报告。受训人员由课程教师从更大的申请者中选择，范围从研究生到高级调查员和研究医生。由于这一课程的时间短，高级和初级人员都可以参加并在一个远离其他时间和注意力要求的环境中接受短期的密集培训。ATMN课程还为已建立的科学家提供了一个不寻常的机会，将这些先进的技术应用于他们自己的研究兴趣，或者在他们可能不熟悉的神经科学中的一个重要专业进行再培训。在课程中教授的方法和概念通过出版实验室手册和在线资源传播到更广泛的研究界。