CSHL Advanced Techniques In Molecular Neuroscience Course

 介绍(由申请人提供)：CSHL分子神经科学高级技术实验室课程拟议中的冷泉港实验室(CSHL)分子神经科学高级技术(ATMN)课程将于2015-2019年7月举行，是CSHL神经生物学研究生培训计划的一部分。ATMN是一门为期两周半的短期强化实践课程，旨在培养学生在当代神经生物学研究中利用先进和/或专门的分子技术进行研究的能力。课程中的实验模块将包括：介绍在神经科学研究中动物病毒载体的设计和使用；使用CRISPR基因组编辑和RNAi方法调控神经元和神经胶质细胞中特定基因的表达；基因传递系统的实践练习，包括哺乳动物细胞感染和体内靶向基因转移的电穿孔技术；BAC转基因载体的总体策略、使用和设计；实时RT-PCR分析；神经元染色质和染色质结构的分析；通过翻译核糖体亲和纯化从指定的神经亚型中分离mRNA。实验技术是在它们可能提供的更广泛的概念进步的背景下教授的。本课程邀请在各自领域做出重大贡献的讲师提供关于当前研究的最新报告。学员是由课程教师从更大的申请者池中挑选出来的，范围从研究生到高级调查人员和研究医生。由于这门课程的持续时间较短，高级和初级学员可以在远离其他时间和注意力需求的环境中参加并接受短期、高强度的培训。ATMN课程还为知名科学家提供了一个不同寻常的机会，让他们将这些先进技术应用于他们自己的研究兴趣，或者再培训他们可能不熟悉的神经科学中的一个重要专业。课程中教授的方法和概念通过出版实验室手册和在线资源向更广泛的研究社区传播。

 DESCRIPTION (provided by applicant): CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2015 - 2019, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive 2.5 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include an introduction to the design and use of animal virus vectors in neuroscience research; the use of CRISPR genome editing and RNAi approaches for regulating the expression of specific genes in neurons and glia; practical exercises in gene delivery systems including mammalian cell infection and transfection and electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; real time RT-PCR analyses; assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by translating ribosome affinity purification. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.