Optimization of CaMPARI for large-scale, cellular-resolution activity recording in freely-moving mice

CaMPARI 的优化，用于自由移动小鼠的大规模细胞分辨率活动记录

• 批准号: 10472700
• 负责人: Robert E. Campbell
• 金额: $ 64.81万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10472700
• 关键词: Affinity; Area; Behavior; Behavior assessment; Benchmarking; Binding; Biological Assay; Brain; Brain region; Calcium; Calcium ion; Cells; Characteristics; Color; Data; Detection; Development; Devices; Dorsal; Ensure; Environment; Equipment; Fluorescence; Future; Genes; Genetic Identity; Goals; Head; Hippocampus; Immunohistochemistry; Implant; In Vitro; Label; Libraries; Light; Lighting; Link; Maps; Measurement; Methods; Microscope; Microscopy; Monitor; Movement; Mus; Mutate; Mutation; Neurons; Noise; Optics; Pathology; Pattern; Performance; Population; Positioning Attribute; Process; Property; Protein Engineering; Proteins; Research; Resolution; Sampling; Signal Transduction; Step Tests; Surface; System; Techniques; Technology; Testing; Tissues; Variant; Viola; Viral Vector; Visual Cortex; advanced system; age related; behavior test; behavioral study; cell type; cognitive testing; cortex mapping; detection sensitivity; experience; experimental study; flexibility; imaging system; implantable device; improved; in vitro Assay; in vitro testing; in vivo; in vivo evaluation; interest; millimeter; miniaturize; neural circuit; novel strategies; optical imaging; optogenetics; predictive test; protein expression; protein purification; response; sample fixation; screening; sensor; single cell sequencing; three photon microscopy; translational neuroscience; two-photon

Project Summary The goal of this proposed research is to optimize a dual-use calcium ion sensor for recording single-cell activity from the entire dorsal cortex or hippocampus of freely-moving mice. It is widely accepted that optical imaging with genetically-encoded fluorescent calcium sensors is currently the only method to obtain measurements of genetically-identified neuronal populations with dense sampling. Over the past several years, the recording capabilities of two-photon microscopes have been improved to record from millimeter-scale tissue, and new miniaturized microscopes have been developed to record from moving mice. However, most behaviors arise from collective interactions between neurons from multiple brain areas, which cannot be simultaneously monitored with these systems. Therefore, there is a clear need to develop a new approach to directly monitor the synchronized activity of distributed neural circuits. CaMPARI is a unique calcium sensor that can detect activity in two calcium-dependent ways: 1) permanent color change (green to red) upon illumination with violet light, a process known as photoconversion, and 2) dynamic changes in fluorescence intensity. Our data show that CaMPARI allows recording of brain activity from freely-behaving mice, without using microscope objectives or implanted devices. Moreover, natural degradation of the red CaMPARI protein enables multiple longitudinal measurements. However, previous attempts to improve CaMPARI using in vitro assays reduced some of its in vivo properties, which resulted in low dynamic recording sensitivity and a low photoconversion rate that requires long illumination times to accumulate a sufficient amount of red protein. Therefore, the project goal is to optimize CaMPARI to allow sensitive recording of cellular-resolution, cortex-wide activity snapshots in freely-moving mice, followed by subsequent dynamic recording from the same mouse using two- and three-photon microscopy. To optimize CaMPARI’s performance, we will combine in vitro testing in purified protein, HEK cells, and neurons, and the most predictive assay: large-scale in vivo screening of ~30-fold more constructs than previous studies. Aim 1 will focus on enhancing CaMPARI’s photoconversion efficiency to facilitate large-scale recordings in freely-moving mice. Aim 2 will focus on improving CaMPARI’s dynamic recording properties and sensitivity. In Aim 3, we will combine beneficial mutations from Aims 1-2 to generate a new CaMPARI with optimized photoconversion and dynamic recording capabilities. Our proof-of-concept experiments will demonstrate multi- regional cortical mapping during a battery of behavioral and cognitive tests to detect cellular-resolution changes in cortex-wide activity patterns. This optimized CAMPARI is expected to facilitate new hypothesis-driven studies by providing volumetric, multi-regional brain activity data of genetically-targeted neurons during cognitive and behavioral testing of freely-moving mice, enabling studies that involve both head-fixation and free movement in the same mice, and to utilize complementary techniques like optogenetic stimulation and single-cell sequencing methods to enable studying the properties of active (red-labeled) cells during behavioral studies.

项目总结