Core 2: Laboratory Services Core

核心 2：实验室服务核心

• 批准号: 10471454
• 负责人: DANIEL S MCCONNELL
• 金额: $ 115.7万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10471454
• 关键词: Address; Affect; Aging; Alzheimer' s Disease; American; Biological; Biological Assay; Biological Markers; Biological Specimen Banks; Cardiac health; Cardiovascular Diseases; Cardiovascular system; Characteristics; Clinic Visits; Clinical; Cognition; Cognitive; Cognitive deficits; Collection; Data Analyses; Data Collection; Dementia; Elderly; Endocrine; Ensure; Event; Fracture; Freezing; Galectin 3; Genitourinary system; Glucose; Goals; Gonadal Steroid Hormones; Guidelines; Health; Health Personnel; Hormonal; Hormones; Hospitals; Impaired cognition; Impairment; Insulin; Interleukin-10; Laboratories; Laboratory Study; Language; Leadership; Life Experience; Ligands; Lipids; Liquid Chromatography; Literature; Longevity; Mass Spectrum Analysis; Measurement; Measures; Menopause; Metabolic; Michigan; Musculoskeletal; Natural History; Osteoporosis; Outcome; Ovarian hormone; Pathologist; Pathology; Pattern; Physical Function; Positioning Attribute; Preparation; Quality of life; Research Personnel; Resources; Risk; Role; Running; Sampling; Science; Services; Sex Functioning; Shipping; Site; Sleep; Sleep disturbances; Specimen; Study of Women' s Health Across the Nation; Testing; Time; Translating; Universities; Vascular Dementia; Visuospatial; Woman; Women' s Health; base; biobank; bone health; cardiometabolism; clinical research site; cognitive function; college; cost; critical period; cytokine; data cleaning; data exchange; data management; dementia risk; design; disability; ethnic difference; falls; health disparity; healthy aging; human old age (65+); instrumental activity of daily living; meetings; metabolomics; middle age; mild cognitive impairment; mortality; multiple chronic conditions; novel; preservation; prevent; psychosocial; psychosocial wellbeing; racial and ethnic; racial and ethnic disparities; repository; sample collection; socioeconomics

SWAN-Aging will test the relation of midlife health and the menopause transition (MT) to successful aging in women. The Laboratory Services Core (Core 2) provides critical support for addressing the scientific aims of three integrated Projects: Project 1 will evaluate how the MT and midlife health relate to preservation of cognitive function (avoiding cognitive decline and onset of mild cognitive impairment), sleep, genitourinary and sexual function, and quality of life in early old age; Project 2 will test how the MT relates to cardiac health and cardiovascular disease (CVD) events in women in early old age and test how cardiac health relates to early markers of physical impairment, Alzheimer’s disease, and vascular dementia at a critical period in the lifespan; and Project 3 will examine the role of the MT and midlife health on musculoskeletal and physical function. Core 2 will be based at The Clinical Ligand Assay Satellite Services (CLASS) Laboratory which will directly support SWAN-Aging aims by coordinating biomarker assays run at CLASS and at two other labs. CLASS has been the Central Laboratory for endocrine and cytokine assays since SWAN’s inception and has assumed responsibility for creating biosample collection kits, coordination of shipping to and from clinical sites, receiving frozen samples, biobanking, and coordinating or performing all metabolic and cardiovascular assays. Core 2 will provide oversight for all laboratory components and will support the Projects by providing reliable and valid measures of sex hormones (by Liquid Chromatography Tandem Mass Spectroscopy), cardio-metabolic health (glucose, insulin, lipids, galectin-3), and biomarkers relevant to multisystem aging (IL-10, NTproBNP). Core 2 will coordinate with the Administrative Core (Core 1) and the Data Collection and Data Management Core (Core 3) to ensure the quality of specimen collections and data transfer, and will: 1) Oversee preparation and transfer of 46,500 new specimens collected at clinic visits and retrieve 16,400 stored samples from the Repository; 2) Conduct high quality assays meeting the standards of CAP and Biorepository guidelines, including more sensitive sex hormone assays; 3) Provide assay results to Core 3 in a timely fashion for data cleaning and analysis; 4) Provide leadership on the interpretation of assay results for scientific aims testing relationships of hormones, cardiovascular markers, and cytokines to CVD events, bone health, sleep, psychosocial well-being, physical function, and multiple domains of cognition to detect cognitive impairment (executive and visual-spatial function, language fluency) and the functional impact of cognitive deficits (cognitively demanding instrumental activities of daily living). Rigorous measurement of the proposed markers over midlife uniquely position SWAN-Aging to test novel predictors of changes in physical and cognitive function and the onset of multi-morbidity, disability, and cognitive impairment in women. Identifying remediable risk factors for dementia risk early in the natural history of cognitive impairment is critical to promoting healthy aging and to preventing dementias, including Alzheimer’s Disease and vascular dementia, in women.

天鹅老龄化将测试中年健康和更年期过渡（MT）的关系，以成功的老龄化妇女。实验室服务核心（核心2）为实现三个综合项目的科学目标提供关键支持：项目1将评估MT和中年健康与认知功能保护的关系（避免认知能力下降和轻度认知障碍的发生）、睡眠、泌尿生殖和性功能以及老年早期的生活质量;项目2将测试MT如何与老年早期妇女的心脏健康和心血管疾病事件相关，并测试心脏健康如何与生命关键时期的身体损伤、阿尔茨海默病和血管性痴呆的早期标志物相关;项目3将研究MT和中年健康对肌肉骨骼和身体功能的作用。核心2将设在临床配体测定卫星服务（CLASS）实验室，该实验室将通过协调在CLASS和其他两个实验室运行的生物标志物测定来直接支持天鹅老化目标。自SWAN成立以来，CLASS一直是内分泌和细胞因子检测的中心实验室，并负责创建生物样本采集试剂盒，协调往返临床研究中心的运输，接收冷冻样本，生物库，以及协调或执行所有代谢和心血管检测。核心2将对所有实验室组成部分进行监督，并将通过提供可靠有效的性激素（通过液相色谱串联质谱法）、心脏代谢健康（葡萄糖、胰岛素、脂质、半乳糖凝集素-3）和多系统衰老相关生物标志物（IL-10、NTproBNP）的测量来支持项目。核心2将与管理核心（核心1）和数据收集和数据管理核心（核心3）协调，以确保样本采集和数据传输的质量，并将：1）监督在临床访视时采集的46，500份新样本的制备和传输，并从储存库中检索16，400份储存样本; 2）进行符合CAP和生物储存指南标准的高质量检测，包括更敏感的性激素检测; 3）及时向Core 3提供检测结果，以进行数据清理和分析; 4）为科学目的的分析结果的解释提供领导，测试激素、心血管标志物和细胞因子与CVD事件、骨骼健康、睡眠、心理社会健康、身体功能的关系，和多个认知领域，以检测认知障碍（执行和视觉空间功能、语言流畅性）以及认知缺陷的功能影响（日常生活中对认知要求很高的工具性活动）。严格测量建议的标志物在中年独特的地位天鹅老化测试新的预测因子的变化，身体和认知功能和发病的多发病率，残疾和认知障碍的妇女。在认知障碍的自然史早期确定痴呆风险的可补救风险因素对于促进健康老龄化和预防痴呆症，包括阿尔茨海默病和血管性痴呆症，在女性中至关重要。