Genome Sequencing Core

基因组测序核心

• 批准号: 10414317
• 负责人: Erik A Lundquist
• 金额: $ 22.95万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10414317
• 关键词: Affect; Animal Model; Area; Biological Sciences; Biology; Centers of Research Excellence; Chromatin; Communication; Custom; DNA Sequencing Facility; DNA sequencing; Data; Development; Discipline; Disease; Disease Pathway; Ecology; Engineering; Experimental Designs; Future; Genome; Genomics; Genotype; Institution; Kansas; Laboratory Research; Length; Libraries; Medical center; Methods; Molecular; Molecular Analysis; Museums; Mutation; Natural History; Pharmacy facility; Phase; Pilot Projects; Positioning Attribute; Preparation; Protocols documentation; Publishing; Research; Research Peer Review; Research Personnel; Research Project Grants; Running; Sequence Analysis; Services; Signal Pathway; Site; Small RNA; Techniques; Technology; Training; Universities; Variant; Wichita; Work; base; chromatin immunoprecipitation; cost; experimental analysis; flexibility; genome sequencing; instrument; instrumentation; interest; next generation sequencing; novel; outreach; programs; tool; transcription factor; transcriptome; transcriptome sequencing; whole genome

PROJECT SUMMARY – CORE B Next generation sequencing is a central tool in the analysis of signaling pathways important in development and disease. The Genome Sequencing Core (GSC) was established in Phase 1 of the CMADP to provide researchers at the University of Kansas and the state of Kansas with next-generation sequencing technologies as well as expertise in experimental design and analysis of sequence data. Projects in the GSC include whole genome assembly, genome re-sequencing for identification of mutations important in development and disease, transcriptome analysis (RNA-seq), variant mapping and genotyping, and identification of transcription factor interaction sites using chromatin immunoprecipitation combined with DNA sequencing (ChIP seq). The GSC houses Illumina MiSeq and NextSeq 550 sequencers with dedicated processor and storage space to run the instruments. To keep the GSC on the cutting edge of technology, a NextSeq 2000 instrument will be installed in the summer of 2021 to enhance and expand services. Next generation sequencing is an enabling technology in that it has the power to allow investigators from many different disciplines to ask new questions in their research areas of interest. The GSC enables genomics research at the University of Kansas by eliminating the barriers of cost and communication involved with using off-campus facilities. The GSC has been utilized by a broad user base at the University of Kansas, including Molecular Biosciences, Ecology and Evolutionary Biology, the Natural History Museum, Engineering, Pharmacy, and the KU Medical Center. Researchers at other universities in Kansas, including Kansas State University, Wichita State University, and Fort Hays State University have used the GSC. The GSC works with other IDeA program projects including the K-INBRE and the CBID COBRE. This broad and diverse user base will be key to the future sustainability of the GSC after Phase 3 as a University Core Research Laboratory.

项目摘要--核心B 下一代测序是分析在发育和发育中重要的信号通路的中心工具 疾病。基因组测序核心(GSC)是在CMADP的第一阶段建立的，以提供 堪萨斯大学和堪萨斯州的研究人员使用下一代测序技术 以及在实验设计和序列数据分析方面的专业知识。GSC中的项目包括 基因组组装，基因组重新测序以识别在发育和疾病中重要的突变， 转录组分析(rna-seq)、变异图谱和基因分型以及转录因子的鉴定 染色质免疫沉淀结合DNA测序的相互作用位点(CHIP SEQ)。GSC 配备Illumina MiSeq和NextSeq 550定序器，配备专用处理器和存储空间，以运行 乐器。为了使GSC保持在技术的前沿，将在 2021年夏季提升和拓展服务。 下一代测序是一项使能技术，因为它有能力让来自许多国家的研究人员 不同的学科在他们感兴趣的研究领域提出新的问题。GSC使基因组学成为可能 堪萨斯大学的一项研究，通过消除使用时涉及的成本和沟通障碍 校外设施。GSC已被堪萨斯大学的广泛用户基础所利用，包括 分子生物科学、生态学和进化生物学、自然历史博物馆、工程学、药学、 和三菱东京大学医疗中心。堪萨斯州其他大学的研究人员，包括堪萨斯州立大学， 威奇托州立大学和海斯堡州立大学已经使用了GSC。GSC与其他想法合作 项目包括K-INBRE和CBID Cobre。这一广泛和多样化的用户基础将是 作为大学核心研究实验室的GSC在第三阶段后的未来可持续性。