Genome Sequencing Core

基因组测序核心

基本信息

批准号：
10654646
负责人：
Erik A Lundquist
金额：
$ 22.95万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-01 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10654646
关键词：
Affect Area Biological Sciences Biology Centers of Research Excellence Chromatin Communication Custom DNA Resequencing DNA Sequencing Facility DNA sequencing Data Dedications Development Discipline Disease Disease Pathway Ecology Engineering Experimental Designs Future Genome Genomics Genotype Institution Kansas Laboratory Research Length Libraries Maps Medical center Methods Molecular Molecular Analysis Museums Mutation Natural History Pharmacy facility Phase Pilot Projects Positioning Attribute Preparation Protocols documentation Publishing Research Research Peer Review Research Personnel Research Project Grants Running Sequence Analysis Services Signal Pathway Site Small RNA Techniques Technology Training Universities Variant Wichita Work base chromatin immunoprecipitation cost experimental analysis flexibility genome sequencing instrument instrumentation interest model organism next generation sequencing novel outreach programs tool transcription factor transcriptome transcriptome sequencing whole genome

项目摘要

PROJECT SUMMARY – CORE B Next generation sequencing is a central tool in the analysis of signaling pathways important in development and disease. The Genome Sequencing Core (GSC) was established in Phase 1 of the CMADP to provide researchers at the University of Kansas and the state of Kansas with next-generation sequencing technologies as well as expertise in experimental design and analysis of sequence data. Projects in the GSC include whole genome assembly, genome re-sequencing for identification of mutations important in development and disease, transcriptome analysis (RNA-seq), variant mapping and genotyping, and identification of transcription factor interaction sites using chromatin immunoprecipitation combined with DNA sequencing (ChIP seq). The GSC houses Illumina MiSeq and NextSeq 550 sequencers with dedicated processor and storage space to run the instruments. To keep the GSC on the cutting edge of technology, a NextSeq 2000 instrument will be installed in the summer of 2021 to enhance and expand services. Next generation sequencing is an enabling technology in that it has the power to allow investigators from many different disciplines to ask new questions in their research areas of interest. The GSC enables genomics research at the University of Kansas by eliminating the barriers of cost and communication involved with using off-campus facilities. The GSC has been utilized by a broad user base at the University of Kansas, including Molecular Biosciences, Ecology and Evolutionary Biology, the Natural History Museum, Engineering, Pharmacy, and the KU Medical Center. Researchers at other universities in Kansas, including Kansas State University, Wichita State University, and Fort Hays State University have used the GSC. The GSC works with other IDeA program projects including the K-INBRE and the CBID COBRE. This broad and diverse user base will be key to the future sustainability of the GSC after Phase 3 as a University Core Research Laboratory.

项目摘要 - 核心B 下一代测序是分析在开发中重要的信号通路的中心工具疾病。基因组测序核心（GSC）在CMADP的阶段1中建立下一代测序技术的堪萨斯大学和堪萨斯州的研究人员以及序列数据实验设计和分析方面的专业知识。 GSC中的项目包括整体基因组组装，重新测序以鉴定在发育和疾病中重要的突变，转录组分析（RNA-SEQ），变体映射和基因分型以及转录因子的鉴定使用染色质免疫沉淀与DNA测序（CHIP SEQ）结合的相互作用位点。 GSC 设有Illumina Miseq和NextSeq 550序列，带有专用处理器和存储空间来运行乐器。为了将GSC保持在技术的最前沿，将安装NextSeq 2000仪器 2021年夏天，以增强和扩展服务。下一代测序是一种促成技术，因为它有能力允许许多人的调查员不同的学科在其感兴趣的研究领域提出新问题。 GSC启用基因组学通过消除使用的成本和沟通障碍，在堪萨斯大学的研究校外设施。 GSC已被堪萨斯大学的广泛用户群使用，包括分子生物科学，生态学和进化生物学，自然历史博物馆，工程，药房，和KU医疗中心。堪萨斯州其他大学的研究人员，包括堪萨斯州立大学，威奇托州立大学和海斯堡州立大学使用了GSC。 GSC与其他想法一起工作计划项目，包括K-Inbre和CBID Cobre。这个广泛而潜水的用户群将是第三阶段后，GSC作为大学核心研究实验室的未来可持续性。