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Genome Sequencing

基因组测序

基本信息

批准号：
10245046
负责人：
Erik A Lundquist
金额：
$ 21.02万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-15 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10245046
关键词：
Affect Animal Model Area Bioinformatics Biological Sciences Biology Biomedical Engineering Biomedical Research Businesses Cell physiology Centers of Research Excellence Chromatin Communication Core Facility Custom DNA Sequencing Facility DNA sequencing Data Development Discipline Disease Disease Pathway Dissection Ecology Elements Experimental Designs Fluorescence Fluorescent Probes Food Generations Genes Genetic Genome Genomics Genotype Goals Infrastructure Institution Iowa Kansas Libraries Location Methods Microarray Analysis Molecular Molecular Analysis Museums Mutation Natural History Network Infrastructure Partner in relationship Pathway interactions Pharmacy Schools Preparation Protocols documentation Research Research Personnel Running Sequence Analysis Signal Pathway Site Small RNA Synthesis Chemistry Techniques Technology Training Universities Variant Washington Wichita Work chromatin immunoprecipitation cost experimental analysis genome sequencing higher education instrument interest next generation sequencing novel novel strategies outreach tool transcription factor transcriptome transcriptome sequencing whole genome

项目摘要

PROJECT SUMMARY Next generation sequencing is now a central tool in the analysis of signaling pathways important in development and disease. The Genome Sequencing Core (GSC) is aimed at providing researchers at the University of Kansas and the state of Kansas with next-generation sequencing technologies as well as expertise in experimental design and analysis of sequence data. Projects in the GSC include whole genome assembly, genome re-sequencing for identification of mutations important in development and disease, transcriptome analysis (RNA seq), variant mapping and genotyping, and identification of transcription factor interaction sites using chromatin immunoprecipitation combined with DNA sequencing (ChIP seq). The GSC houses an Illumina Hiseq 2500 sequencer and a MiSeq sequencer with dedicated processor and storage space to run the instrument. This core facility has enhanced the genomics infrastructure already at KU, in the KU Genomics Center and the KU DNA Sequencing Facility, which provides standard, first generation sequencing. Together, the three cores allow for a thorough, integrated dissection of disease pathways using novel fluorescence probes to define a cellular process, to find mutations affecting that cellular process, and to define genes involved in the cellular process. The novel approach of the CMADP is to combine the enabling technologies of fluorescent probe synthetic chemistry and next generation sequencing to set up a pipeline for target discovery in disease pathways. Next generation sequencing is an enabling technology in that it has the power to allow investigators from many different disciplines to ask new questions in their research areas of interest. The GSC enables genomics research at the University of Kansas by eliminating the barriers of cost and communication involved with using off-campus facilities. Interest in next generation sequencing at the University of Kansas is high, and includes investigators in the Pharmacy School, the Department of Molecular Biosciences, and the Department of Ecology and Evolutionary Biology. Use of the GSC will be by Projects in the COBRE Center for Molecular Analysis of Disease Pathways, as well as by researchers across the State of Kansas and in many different disciplines.

项目摘要下一代测序现在是分析信号通路的核心工具，发展和疾病。基因组测序核心（GSC）旨在为研究人员提供堪萨斯大学和堪萨斯州的下一代测序技术，以及实验设计和序列数据分析方面的专业知识。GSC的项目包括全基因组组装，基因组重新测序，用于鉴定在发育和疾病中重要的突变，转录组分析（RNA seq）、变体作图和基因分型以及转录因子的鉴定使用染色质免疫沉淀结合DNA测序（ChIP seq）检测相互作用位点。则GSC 包含Illumina Hiseq 2500测序仪和MiSeq测序仪，配有专用处理器和存储器空间来运行仪器。这一核心设施增强了KU基因组学中心的基因组学基础设施， KU DNA测序设施，提供标准的第一代测序。在一起，三个核心允许使用新的荧光探针对疾病途径进行彻底的、综合的解剖，定义一个细胞过程，找到影响细胞过程的突变，并定义参与细胞过程的基因。细胞过程CMADP的新方法是联合收割机结合荧光探针合成化学和下一代测序，为疾病中的靶点发现建立管道途径。下一代测序是一种使能技术，因为它有能力让研究人员从许多不同的学科在他们感兴趣的研究领域提出新的问题。GSC使基因组学研究在堪萨斯大学通过消除成本和沟通的障碍，涉及使用校外设施堪萨斯大学对下一代测序的兴趣很高，包括研究人员在药学院，分子生物科学系，和系生态学与进化生物学GSC将由COBRE分子中心的项目使用疾病途径的分析，以及堪萨斯州和许多不同地区的研究人员学科