Core B: Analytical & Synthetic Chemistry Core
核心 B:分析
基本信息
- 批准号:10653040
- 负责人:
- 金额:$ 21.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-01 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcetatesAnalytical ChemistryAnimal ModelAnimalsBetaineBiochemicalBiologicalBiological AssayButyratesCarbonCardiometabolic DiseaseCardiovascular DiseasesCarnitineCellular biologyChemicalsCholineClinicClinicalCost SavingsCoupledData AnalysesDetectionDevelopmentEducationElementsEnzyme Inhibitor DrugsEnzymesEquipmentGas ChromatographyGenerationsGoalsHandHigh Pressure Liquid ChromatographyInstitutionIsomerismIsotope LabelingIsotopesKnowledgeLaboratoriesLiquid ChromatographyLiquid substanceLyaseMass Spectrum AnalysisMeasurementMetabolic DiseasesMethodsModernizationMolecular BiologyMultinuclear NMRNutrientPathway interactionsPatternPropionatesProtonsResearch DesignResearch PersonnelResolutionSamplingServicesSiteSourceSpectroscopy, Fourier Transform InfraredStructureStudentsStudy modelsSuccinatesSynthesis ChemistrySystemTechniquesTimeTissuesTracerTrainingTraining and EducationUniversitiesValidationVolatile Fatty AcidsVolatilizationWait Timeanalytical toolassay developmentcandidate identificationchemical synthesisdesigndetectorexperienceexperimental studygut microbiotain vivoinhibitorinstrumentationinterestinvestigator traininglight scatteringmetabolomicsmethod developmentmicrobialoperationprogramssmall moleculestable isotopesuccesssuicide substratestandem mass spectrometrytooltrimethyloxamine
项目摘要
Analytical and Chemical Synthesis Core (Core B): Abstract
The overall goals of the Analytical and Chemical Synthesis Core are to provide a diverse suite of
analytical and organic synthetic chemical services. The core will provide unique access to a large variety
of mass spectrometry assays, assay development expertise, as well as chemical synthesis support services for
compound identification, provision of synthetic microbial enzyme inhibitors, and synthesis of isotope labeled
standards and precursor molecules.
This core will provide multiple analytical services in support of all Projects in the PPG. Moreover, all Projects
rely heavily on mass spectrometry as both an investigative/discovery tool, and as a workhorse for quantitative
analyses of biological and clinical samples. Mass spectrometry support includes both targeted and untargeted
mass spectrometry platforms using as interface either liquid chromatography (LC) or gas chromatography (GC)
systems, depending on experiment and analyte requirements. Compound identification services include both
high resolution mass spectrometry platform capabilities, as well as chemical isolation, and multinuclear NMR
platforms. Following untargeted mass spectrometry analyses and candidate metabolite identification,
quantitative stable isotope dilution tandem mass spectrometry assays will be developed for metabolites of
interest to the Projects. If the candidates are not available in commercial form (unlabeled or isotope labeled),
they will be chemically synthesized as needed by the synthetic support services provided by the core. The core
also offers a broad suite of quantitative LC/MS/MS assays of panels of gut microbiota generated metabolites,
and GC/MS analyses of volatile analytes relevant to gut microbiota studies (e.g. short chain fatty acids). Heavy
isotope labeled precursor compounds for isotope tracer studies (both in culture and for provision to animals),
and synthesis of newly developed gut microbial enzyme inhibitors (cutC mechanism based inhibitors) will also
be provided in sufficient quantity by the chemical synthesis support services of the core for animal model
studies. The Core will also assist all Projects and Cores in the PPG in obtaining material for analysis and
verification of analyte identities in each Project. Finally, the core will serve as a training hub for PPG
investigators and students, providing expertise in operation of analytical and various other chemical equipment,
methods development, study design, and oversight in use and operation of analytical equipment.
分析和化学合成核心(核心B):摘要
分析和化学合成核心的总体目标是提供一套多样化的
分析和有机合成化学服务。核心将提供独特的途径,以获得大量的
质谱学分析、分析开发专业知识以及化学合成支持服务
化合物鉴定、合成微生物酶抑制剂的提供和同位素标记的合成
标准品和前体分子。
这一核心将提供多种分析服务,以支持PPG的所有项目。此外,所有项目
严重依赖质谱学作为研究/发现工具,也是定量分析的主力
生物样品和临床样品的分析。质谱学支持包括定向和非定向
使用液相色谱(LC)或气相色谱(GC)作为界面的质谱仪平台
系统,取决于实验和分析物要求。复合身份识别服务包括这两项
高分辨率质谱学平台能力,以及化学分离和多核核磁共振
站台。在非靶向质谱分析和候选代谢物鉴定之后,
将建立稳定的稳定同位素稀释串联质谱分析方法
对这些项目感兴趣。如果不能以商业形式(未标记或同位素标记)获得候选,
它们将根据需要由核心提供的合成支助服务进行化学合成。其核心是
还提供一套广泛的肠道微生物区系代谢产物的LC/MS/MS定量分析方法,
以及与肠道微生物区系研究相关的挥发性分析物的GC/MS分析(例如短链脂肪酸)。重的
用于同位素示踪研究的同位素标记前体化合物(在培养中和提供给动物),
而新开发的肠道微生物酶抑制剂(基于CutC机制的抑制剂)的合成也将
由动物模型核心的化学合成支持服务提供充足的数量
学习。核心还将协助PPG中的所有项目和核心获得材料以供分析和
验证每个项目中的分析物身份。最后,核心将作为PPG的培训中心
研究人员和学生,提供分析和各种其他化学设备的操作专业知识,
方法分析仪器的开发、研究设计、使用和操作中的监督。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Zeneng Wang的其他文献
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{{ truncateString('Zeneng Wang', 18)}}的其他基金
Microbial Trimethylamine Lyases and Atherosclerosis
微生物三甲胺裂解酶和动脉粥样硬化
- 批准号:
9006675 - 财政年份:2016
- 资助金额:
$ 21.11万 - 项目类别:
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