The experience of human subjects with brain organoid research

人类受试者进行脑类器官研究的经验

• 批准号: 10660220
• 负责人: Kate E. MacDuffie
• 金额: $ 24.9万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10660220
• 关键词: 3-Dimensional; Address; Alzheimer' s Disease; Animal Model; Animals; Attitude; Award; BRAIN initiative; Back; Base of the Brain; Basic Science; Belief; Belief System; Bioethics; Biological; Brain; Brain Diseases; Cell Line; Cell model; Cells; Child; Childhood; Clinical; Clinical Data; Code; Communication; Consent Forms; DNA; Data; Data Collection; Development; Disease; Ensure; Environment; Ethical Issues; Ethics; Faculty; Family; Family member; Functional disorder; Future; Genes; Goals; Human; Imaging Techniques; In Vitro; Individual; Informed Consent; Interview; Joubert syndrome; Kidney; Knowledge; Laboratories; Learning; Link; Lung; Master' s Degree; Measures; Mentors; Modeling; Modification; Morals; Nerve Degeneration; Neurodegenerative Disorders; Neurodevelopmental Disorder; Neurosciences; Neurosciences Research; Organ; Organoids; Participant; Pathology; Patients; Pattern; Phase; Play; Policies; Positioning Attribute; Process; Protocols documentation; Registries; Research; Research Training; Role; Sampling; Scientist; Specimen; Structure; Surveys; Technology; Time; Tissues; Training; Universities; Washington; autism spectrum disorder; biobank; brain behavior; career; clinical application; efficacy testing; experience; human model; human subject; improved; in vitro Model; induced pluripotent stem cell; innovation; insight; multidisciplinary; nervous system disorder; neural network; neurodevelopment; neuroethics; neurotechnology; non-invasive imaging; pilot test; preference; relating to nervous system; skills; sociodemographic factors; sociodemographics; stakeholder perspectives; stem cells; surrogate decision maker; three-dimensional modeling; working group

PROJECT SUMMARY Efforts to understand the mechanisms of brain-based disease have been hindered by the limited ability of animal models to reflect the full complexity of human brain and behavior. Brain organoids represent a potential solution, allowing scientists to model human neurodevelopmental and disease processes in-vitro. These multicellular, three-dimensional tissue structures are derived from induced pluripotent stem cells and self-organize to recapitulate aspects of human cortical development. Brain organoids are linked to their original human donor through shared DNA, and also through what they can reveal about individual-specific patterns of brain development and dysfunction. Given the central role the brain plays in personal identity, it is critical to understand what human donors think and feel about brain organoids grown from their cells, and consider whether organoid models of the brain deserve unique moral status compared to, for example, lung or kidney organoids. The proposed research seeks to engage participants and other stakeholders to explore ethical issues in brain organoid research, and thus addresses a core principle of BRAIN Initiative: considering the ethical implications of neuroscience research. In Aim 1, qualitative interviews with current participants will be conducted to explore their perspectives towards brain organoid research including beliefs about meaning, moral status, and permissible uses of organoid tissue. In Aim 2, an educational video that describes brain organoids will be imbedded into an online survey and used to measure attitudes in larger groups of stakeholders who represent future participants in brain organoid research. In Aim 3, insights learned from participants and stakeholders about preferences for brain organoid research will be compared to current research practices and modifications to guide the field going forward will be proposed. The long-term goal is to ensure that brain organoid research can achieve its scientific potential and proceed in accordance with donor beliefs and values. The proposed research and training plan will leverage expertise of an interdisciplinary team of mentors and strong bioethics/neuroethics research environment across the University of Washington Center for Neurotechnology and the Treuman Katz Center for Pediatric Bioethics at Seattle Children’s. During the mentored phase of the award, the applicant will gain new content knowledge in the basic science of brain organoids, learn new skills for neuroscience communication, and complete a master’s degree in bioethics. This additional training will equip the applicant with the skills necessary to conduct the proposed research and transition into an independent faculty position.

项目摘要 有限的 动物模型反映人脑和行为的全部复杂性的能力。脑器官 代表潜在的解决方案，使科学家能够对人类神经发育和 疾病过程中。这些多细胞的三维组织结构被得出 从诱导的多能干细胞和自组织以概括人皮质方面 发展。脑器官通过共享DNA与其原始的人类供体有关，并且 还可以通过他们可以揭示有关大脑发育的个体特定模式和 功能障碍。鉴于大脑在个人身份中扮演的核心作用，了解 人类捐助者对从细胞中生长的脑类器官的想法和感受，并考虑 与 肺或肾脏器官。拟议的研究旨在吸引参与者和其他 利益相关者探索脑器官研究中的道德问题，从而解决核心 大脑倡议原则：考虑神经科学研究的道德意义。目标 1，将对当前参与者进行定性访谈以探索他们的观点 朝大脑器官研究，包括对意义，道德地位和许可的信念 器官组织的使用。在AIM 2中，描述脑器官的教育视频将是 嵌入到在线调查中，并用于衡量大型利益相关者的参与者 代表未来的脑器官研究参与者。在AIM 3中，从中学到的见解 将参与者和利益相关者将有关脑器官研究偏好的偏好进行比较 将提出当前的研究实践和修改以指导未来的领域。 长期目标是确保大脑器官研究能够发挥其科学潜力 并根据捐助者的信念和价值观进行。拟议的研究和培训 计划将利用跨学科的导师团队和强大的专业知识 华盛顿大学中心的生物伦理学/神经伦理学研究环境 西雅图儿童的神经技术和小儿生物伦理学中心。 在奖励的指导阶段，申请人将获得新的内容知识 脑器官的基础科学，学习神经科学沟通的新技能，并完成 生物伦理学硕士学位。这种额外的培训将使申请人具备技能 进行拟议的研究和过渡到独立的教师职位所必需的。