2022 CCS Oral Microbiome and Pulmonary Function Supplement

抽象的。2022财年，我们要求OAR战略基金对 口腔微生物群、免疫反应和HIV与慢性阻塞性肺疾病的关系 肺部疾病(COPD)。慢性阻塞性肺疾病，包括呼吸道阻塞和肺气肿，是一种 即使在目前的抗逆转录病毒时代，艾滋病毒携带者(PWH)的健康问题也在增加 治疗(ART)。我们的初步数据表明，口腔微生物群与肺部有关。 功能，但我们缺乏对特定微生物群落作用的详细了解 以及支撑这种联系的潜在机制，这目前阻碍了 制定干预策略。我们将利用口腔数据，微生物组分析，血液 样本和肺功能测量已经在整个MWCCS中收集 访问102和103，以分析口腔健康和口腔微生物群之间的关系 肺功能。我们还将进行外周细胞因子的测量，炎症性 反应，和口腔环境，以评估潜在的机制联系 微生物群对肺部健康的影响。这些调查将在所有符合以下条件的个人中进行 完成了MWCCS的肺功能测试。然后我们将进行详细的口头表演 评估和收集参与者子组中多个口腔壁龛的微生物样本 更好地描述PIT和PIT的特定口腔健康成分与肺功能的关系 北卡罗来纳大学。使用一种新技术来鉴定免疫球蛋白结合的细菌和真菌，我们将 确定驱动宿主反应并与慢性阻塞性肺疾病相关的关键微生物。这些调查 将确定导致威斯康星医院慢性阻塞性肺病的潜在可修改目标。 我们将解决NIH高度优先的艾滋病指定主题：1)常见和高负担的艾滋病毒- 相关的共病；2)肺部疾病专家之间的跨学科合作， 微生物组、牙科医学和分析；3)由于社会的影响，艾滋病毒的差异 健康对肺功能和口腔健康的决定因素；4)交叉研究的基本发现 尖端科学；以及5)培训下一代艾滋病毒调查人员。

Abstract. FY 2022 OAR Strategic Funds are requested to perform a detailed investigation of the relationship of the oral microbiome, immune response, and HIV to chronic obstructive pulmonary disease (COPD). COPD, including airway obstruction and emphysema, is an increasing health problem in people with HIV (PWH) even in the current era of antiretroviral therapy (ART). Our preliminary data suggest that the oral microbiome is associated with lung function, but we lack a detailed understanding of the role of particular microbial communities and the potential mechanisms underpinning the association, which currently impedes development of intervention strategies. We will utilize oral data, microbiome analyses, blood specimens, and pulmonary function measures already being collected throughout MWCCS in visits 102 and 103 in order to analyze the relationship of oral health and the oral microbiome to lung function. We will also perform measurements of peripheral cytokines, inflammatory responses, and the oral cavity environment to assess potential mechanisms linking the microbiome to lung health. These investigations will be performed in all individuals who have completed pulmonary function testing across MWCCS. We will then perform detailed oral evaluations and collect microbial samples from multiple oral niches in a subset of participants to better delineate relationships of specific oral health components and lung function at Pitt and UNC. Using a novel technique to identify immunoglobulin-bound bacteria and fungi, we will identify key microbes that drive host response and are related to COPD. These investigations will identify potentially modifiable targets contributing to COPD in PWH. We will address NIH high priority AIDS-designated topics of: 1) a common and high-burden HIV- associated comorbidity; 2) interdisciplinary collaborations among experts in pulmonary disease, microbiome, dental medicine, and analytics; 3) disparities in HIV given impact of social determinants of health on both lung function and oral health; 4) fundamental discovery in cross- cutting science; and 5) training the next generation of HIV investigators.