3D Bioprinted Omentum tissue to model ovarian cancer metastasis

3D 生物打印网膜组织用于模拟卵巢癌转移

• 批准号: 10683668
• 负责人: Marc Ferrer
• 金额: $ 90.89万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10683668
• 关键词: 3-Dimensional; Animal Model; Antineoplastic Agents; Biological Assay; Blood Vessels; Cancer Cell Growth; Cell Line; Cell model; Cell-Matrix Junction; Cells; Development; Endothelial Cells; Fibroblasts; Fluorescence Microscopy; Functional disorder; Genetically Engineered Mouse; Goals; Growth; Human; Immunodeficient Mouse; In Vitro; Label; Location; Malignant Neoplasms; Malignant neoplasm of ovary; Mesothelial Cell; Metastatic Malignant Neoplasm to the Ovary; Modeling; Monitor; Morphology; Neoplasm Metastasis; Omentum; Patients; Pericytes; Physiological; Physiology; Preclinical Testing; Production; Protocols documentation; Site; Surface; Suspensions; Three-Dimensional Imaging; Tissues; Toxicity Tests; Xenograft procedure; biofabrication; bioprinting; cancer cell; cell growth; cellular imaging; clinical efficacy; drug development; drug discovery; efficacy testing; monolayer; neoplastic cell; novel therapeutics; ovarian neoplasm; predictive test; prevent; screening; tumor

We have developed biofabrication protocols for the production of a vascularized omentum model that includes patient derived fibroblasts, endothelial cells and pericytes, and a layer of patient mesothelial cells. Patient cells were obtained from our collaborators Dr. Ernst Lengyel and Hilary Kenny. Addition of a suspension of fluorescently labeled ovarian tumor cells to the top of mesothelial cell layer on the tissue and used fluorescence microscopy to monitor tumor formation inside the tissue. 3D imaging analysis have been developed to quantiate size and number of tumors, and their location. These analytics will be used to screen a focused set of compounds for compounds that prevent tumor formation in the tissue.

我们已经开发了生物制造方案，用于生产包括患者衍生的成纤维细胞，内皮细胞和周细胞以及一层患者间皮细胞的血管化足体模型。 从我们的合作者Ernst Lengyel博士和Hilary Kenny获得患者细胞。 将荧光标记的卵巢肿瘤细胞的悬浮液添加到组织上的间皮细胞层顶部，并使用荧光显微镜监测组织内部的肿瘤形成。 已经开发出3D成像分析以量化肿瘤的大小和数量及其位置。 这些分析将用于筛选一组聚焦的化合物，以防止组织中肿瘤形成。