The need for speed: Stimulated Raman Spectroscopy for human gut microbiome research

我请求对我的R35 MIRA奖进行补充，以帮助获得受激拉曼的激光源 显微镜，以帮助我的实验室的研究开发新的显微镜工具，为人类肠道微生物组 research.在我最初的MIRA应用程序中，为人类开发下一代生理学方法 肠道微生物组研究（奖项1 R35 GM 147166 -01），我描述了强大的，但目前未充分利用 拉曼显微光谱（拉曼）用于人类肠道微生物组研究的潜力。我特别 解决了如何自发拉曼显微镜将用于研究方向2和3，这将 结合联合收割机稳定同位素探测和拉曼来探测微生物原位功能（方向2）并将其与 拉曼与其他显微技术，以获得更全面的了解肠道微生物（方向 3）。受激拉曼光谱提供比自发光谱快1，000 - 1，000，000倍的采集速率 拉曼光谱，但直到最近还没有商业化;当徕卡推出 第一台推向市场的自发拉曼显微镜。这台仪器的能力将允许光谱 采集量将增加1，000倍，这将使结肠镜检查和其他人类肠道检查成为可能 微生物组样本将在几分钟内以亚细胞空间分辨率进行化学映射。此外，本发明还提供了一种方法， 这将使我的实验室能够扩大我们的研究，利用微生物细胞特性和合成代谢活性， 振动探针，这是不可能使用目前的自发拉曼技术，因此没有 在我最初的MIRA申请中。如果获奖，这款乐器将成为第三个受激励的乐器。 拉曼显微镜在全国任何一所大学。

I request a supplement to my R35 MIRA award to help acquire the laser source for a Stimulated Raman Microscope to aid my lab’s research on developing new microscopy tools for human gut microbiome research. In my original MIRA application, Developing Next-Generation Physiology approaches for human gut microbiome research (award 1R35GM147166-01), I described the strong but currently underutilized potential of Raman microspectroscopy (Raman) for human gut microbiome research. Specifically, I addressed how Spontaneous Raman microscopy will be used in research directions 2 and 3, which will combine stable isotope probing and Raman to probe microbial in situ function (direction 2) and correlate Raman with other microscopic techniques to gain a more holistic understanding of gut microbes (direction 3). Stimulated Raman spectroscopy offers 1,000-1,000,000 faster acquisition rates than Spontaneous Raman spectroscopy, but until recently was not commercially available; this changed when Leica brought the first Spontaneous Raman microscope to market. The capabilities of this instrument will allow spectral acquisition to be increased by a factor of 1,000, which will enable colonoscopy and other human gut microbiome samples to be chemically mapped at subcellular spatial resolution within minutes. In addition, it will allow my lab to expand our research to visualize microbial cell identity and anabolic activity using vibrational probes, which is not possible using currently Spontaneous Raman technology and thus was not included in my original MIRA application. If awarded, this instrument will become the 3rd Stimulated Raman microscope at any university in the nation.