Development of Sensitive and Specific Proteomic Biomarkers of Aging, Health, Frailty, and Morbidity in Human Cohorts

基于我们先前开发衰老生物标志物特征的努力，我们将努力集中在发现单核细胞中发现的一组新的衰老生物标志物上，单核细胞是一种既接近血液又已知随着人类年龄增长而衰老的细胞类型。在过去的一年中，我们建立了一个衰老的单核细胞培养，并进行了大规模的蛋白质组学分析衰老的人单核细胞。此外，为了测试这组细胞类型特异性生物标志物及其与人类衰老和疾病的关系，我们正在几个队列中启动研究：GESTALT，BLSA和InCHIANTI，以将一组新的生物标志物候选物与人类健康，虚弱和其他临床参数相关联。 该研究项目的目的和范围是开发和应用最先进的基于质谱的蛋白质组学技术，以发现和验证代表人体组织，血液和其他生物流体中衰老和疾病负担的蛋白质和蛋白质形式的子集。

Building on our previous efforts to develop senescence biomarker signatures, we have focused our efforts on the discovery of a novel set of senescence biomarkers found in monocytes, a cell type that is both proximal to the blood and known to increase in senescence with age in humans. In the past year, we have established a senescent monocyte culture and performed large-scale proteomic analysis of senescent human monocytes. Further, to test this cell-type specific set of biomarkers and its relationship to aging and diseases in humans, we are initiating studies in several cohorts: GESTALT, BLSA, and InCHIANTI, to correlate a new set of biomarker candidates with human health, frailty, and other clinical parameters. The purpose and scope of this research project going forward is to develop and apply state-of-the-art mass spectrometry based proteomic techniques to discover and validate subsets of proteins and protein forms that are representative of aging and disease burdens in human tissues, blood, and other biofluids.