Understanding Risk of Zoonotic Virus Emergence in EID Hotspots of Southeast Asia

了解东南亚 EID 热点地区人畜共患病毒出现的风险

• 批准号: 10708750
• 负责人: Peter Daszak
• 金额: $ 146.47万
• 依托单位: ECOHEALTH ALLIANCE, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-17 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10708750
• 关键词: Animal Model; Animals; Archives; Asia; Asian; Behavioral; Biological; Biological Assay; Biology; Cell Culture Techniques; China; Chiroptera; Clinic; Clinical; Clinical Management; Collaborations; Collection; Communicable Diseases; Communities; Coronavirus; Country; Dangerousness; Data; Data Analyses; Demography; Development; Disease; Disease Outbreaks; Emerging Communicable Diseases; Encephalitis; Enrollment; Epidemiology; Etiology; Event; Exposure to; Family; Family suidae; Far East; Frequencies; Geography; Goals; Hospitals; Human; Human Resources; In Vitro; Infection; Infectious Diseases Research; Influenza; Institution; International; Investigation; Laboratories; Leadership; Link; Livestock; Malaysian; Mammals; Metadata; Modeling; Molecular; Mus; Nipah Virus; Occupational; Pathogenicity; Pathway interactions; Patients; Persons; Phylogenetic Analysis; Population; Primates; Probability; Questionnaires; Reagent; Research; Research Institute; Research Personnel; Resources; Risk; Risk Assessment; Risk Factors; Rodent; Rural Community; Sampling; Scientist; Series; Serodiagnoses; Serology; Serology test; Severe Acute Respiratory Syndrome; Singapore; Southeastern Asia; Surveys; Symptoms; Syndrome; System; Taxonomy; Technology; Testing; Thailand; Translational Research; Viral; Virus; Work; Zoonoses; cohort; community clinic; design; diagnostic assay; experience; experimental study; follow-up; fundamental research; high risk; human data; humanized mouse; in vivo; influenza outbreak; influenza virus strain; intervention program; multidisciplinary; new outbreak; novel; novel coronavirus; novel virus; pandemic disease; pandemic potential; participant enrollment; pathogen; pathogenic virus; rapid detection; receptor binding; respiratory; scale up; serosurvey; skills; southeast Asian; syndromic surveillance; transmission process

PROJECT SUMMARY/ABSTRACT Southeast Asia is one of the world’s highest-risk EID hotspots, the origin of the SARS pandemic, Nipah virus, and repeated outbreaks of influenza. This is driven by high diversity of wildlife and rapidly expanding demography that brings human and wildlife populations closer. This proposal will launch the Emerging Infectious Diseases - South East Asia Research Collaboration Hub (EID-SEARCH), a collaboration among leaders in emerging disease research in the USA, Thailand, Singapore and the 3 major Malaysian administrative regions. These researchers have networks that span >50 clinics, laboratories and research institutions across almost all SE Asian countries and will use the EID-SEARCH as an early warning system for outbreaks involving exchange of information, reagents, samples and technology, and a collaborative power- house for fundamental and translational research. The EID-SEARCH will also act as a significant asset to scale-up and deploy resources in the case of an outbreak in the region. This EIDRC will conduct research to: 1) Identify, characterize and rank spillover risk of high zoonotic potential viruses from wildlife, by analyzing previously-archived wildlife samples, conducting targeted wildlife surveillance, and using serology & PCR assays to identify novel viruses. These will be characterized to assess risk of spillover to people, and a series of in vitro (receptor binding, cell culture) and in vivo (humanized mouse and collaborative cross models) assays used to assess their potential to infect people and cause disease; 2) Collect samples and questionnaire data from human communities that live in EID hotspots and have high cultural and behavioral risk of animal exposure (e.g. wildlife hunting, bat guano collection). These will be tested with serological assays to identify evidence of novel virus spillover, and analyzed against metadata to identify key risk pathways for transmission; 3) Identify and characterize viral etiology of ‘cryptic’ outbreaks in clinical cohorts. We will conduct syndromic surveillance at clinics serving the populations in Aim 2, enroll patients with undiagnosed illness and symptoms consistent with emerging viral pathogens, and test samples with molecular and follow-up serological assays to identify causal links between these syndromes and novel viruses. This research will advance our understanding of the risk of novel viral emergence in a uniquely important region. It will also strengthen in-country research capacity by linking local infectious disease scientists with an international collaborative network that has proven capacity to conduct this work and produce significant findings. The large body of high impact collaborative research from this EIDRC leadership team provides proof-of-concept that EID-SEARCH has the background, collaborative network, experience, and skillset to act as a unique early warning system for novel EIDs of any etiology threatening to emerge in this hottest of the EID hotspots.

项目摘要/摘要 东南亚是世界上最高风险的EID热点之一，是SARS大流行，Nipah病毒的起源， 并反复爆发影响力。这是由野生动植物多样性和迅速扩张的驱动 使人类和野生动植物种群更加接近的示意图。该建议将启动新兴 传染病 - 东南亚研究合作中心（EID -Search），这是一种合作 美国，泰国，新加坡和3个主要马来西亚的新兴疾病研究领导者 行政区域。这些研究人员的网络超过50个诊所，实验室和研究 几乎所有东南亚国家的机构 涉及信息，试剂，样品和技术的爆发爆发以及协作权力 - 基础研究和翻译研究之家。 eid-search还将充当重要资产 在该地区爆发的情况下，扩大和部署资源。这个EIDRC将进行研究 TO：1）通过分析仪，野生动植物的高人动势病毒的Spilover风险识别，表征和排名 以前构成的野生动植物样本，进行针对性的野生动植物监视，并使用血清学和PCR 鉴定新病毒的测定法。这些将被特征在于评估对人的Spilover的风险，以及一系列 体外（受体结合，细胞培养）和体内（人源化小鼠和协作横型）测定法 用于评估他们感染人并引起疾病的潜力； 2）收集样品和问卷数据 来自居住在开斋节中并且具有高文化和行为风险的人类社区 暴露（例如，野生动植物狩猎，蝙蝠鸟粪收集）。这些将通过血清学评估进行测试，以识别 新型病毒Spilover的证据，并针对元数据进行分析以识别传播的关键风险途径； 3）识别和表征临床队列中“隐秘”暴发的病毒病因。我们将进行综合征 在AIM 2中为人群提供服务的诊所的监视，招募无诊断疾病和症状的患者 与新兴的病毒病原体一致，并具有分子和随访的血清学测定样品 确定这些综合征与新病毒之间的因果关系。 这项研究将促进我们对独特重要的新型病毒出现风险的理解 地区。它还将通过联系当地传染病科学家来增强国内研究能力 拥有一个国际协作网络，该网络已证明可以进行这项工作并生产 重要的发现。这个EIDRC领导团队的大量高影响力协作研究 提供概念验证，即eid-search具有背景，协作网络，经验和 技能集成为任何威胁要出现的任何病因的新型EID的独特预警系统 这最热的热点。

项目成果

Early detection of neutralizing antibodies against SARS-CoV-2 in COVID-19 patients in Thailand.

• DOI: 10.1371/journal.pone.0246864
• 发表时间: 2021
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Putcharoen O;Wacharapluesadee S;Chia WN;Paitoonpong L;Tan CW;Suwanpimolkul G;Jantarabenjakul W;Ruchisrisarod C;Wanthong P;Sophonphan J;Chariyavilaskul P;Wang LF;Hemachudha T
• 通讯作者: Hemachudha T

A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand.

• DOI: 10.1186/s13071-021-05047-z
• 发表时间: 2021-11-08
• 期刊: Parasites & vectors
• 影响因子: 3.2
• 作者: Phumee A;Wacharapluesadee S;Petcharat S;Siriyasatien P
• 通讯作者: Siriyasatien P

Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia.

• DOI: 10.1038/s41467-021-21240-1
• 发表时间: 2021-02-09
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Wacharapluesadee S;Tan CW;Maneeorn P;Duengkae P;Zhu F;Joyjinda Y;Kaewpom T;Chia WN;Ampoot W;Lim BL;Worachotsueptrakun K;Chen VC;Sirichan N;Ruchisrisarod C;Rodpan A;Noradechanon K;Phaichana T;Jantarat N;Thongnumchaima B;Tu C;Crameri G;Stokes MM;Hemachudha T;Wang LF
• 通讯作者: Wang LF

Limited and Short-Lasting Virus Neutralizing Titers Induced by Inactivated SARS-CoV-2 Vaccine.

• DOI: 10.3201/eid2712.211772
• 发表时间: 2021-12
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Hunsawong T;Fernandez S;Buathong R;Khadthasrima N;Rungrojchareonkit K;Lohachanakul J;Suthangkornkul R;Tayong K;Huang AT;Klungthong C;Chinnawirotpisan P;Poolpanichupatam Y;Jones AR;Lombardini ED;Wacharapluesadee S;Putcharoen O
• 通讯作者: Putcharoen O

Homologous or Heterologous COVID-19 Booster Regimens Significantly Impact Sero-Neutralization of SARS-CoV-2 Virus and Its Variants.

• DOI: 10.3390/vaccines10081321
• 发表时间: 2022-08-15
• 期刊: VACCINES
• 影响因子: 7.8
• 作者: Buathong, Rome;Hunsawong, Taweewun;Wacharapluesadee, Supaporn;Guharat, Suriya;Jirapipatt, Ratthapat;Ninwattana, Sasiprapa;Thippamom, Nattakarn;Jitsatja, Anusara;Jones, Anthony R.;Rungrojchareonkit, Kamonthip;Lohachanakul, Jindarat;Suthangkornkul, Rungarun;Tayong, Kedsara;Klungthong, Chonticha;Fernandez, Stefan;Putcharoen, Opass
• 通讯作者: Putcharoen, Opass