Multi-Omics Core

项目总结(多组学核心) 单细胞转录学是精密人类疼痛网络的核心组成部分，因为这些 强大的新工具为了解细胞类型、分子和细胞提供了前所未有的机会- 导致慢性疼痛的细胞相互作用。多元组学核心将由伦塔尔博士领导并集中 单细胞转录、表观基因组和下一代测序工作与经验丰富的团队在 单细胞和下一代测序工作的开发、优化和分析。这个 这些尖端技术的集中化将确保已建立的协议能够应用 系统地向该中心的所有样本提供信息。具体来说，核心将提供单细胞和单核 在项目1和项目2中都提供多组学服务来表征疼痛相关组织。然后他们将提供这两个 计划快速的下一代测序服务，按成本计算需要1-3天的周转时间。最后，核心将 保持在快速发展的单细胞基因组学领域的前沿，并继续带来创新 从而提高质量，降低成本，回到中心。核心还将与类似的核心进行协调 U19 Precision Human Pain Network协调协议和最佳实践。

PROJECT SUMMARY (Multi-omics Core) Single-cell transcriptomics is a central component of the PRECISION Human Pain Network because these powerful new tools provide unprecedented opportunities for understanding the cell types, molecules, and cell- cell interactions that contribute to chronic pain. The Multi-omics Core will be led by Dr. Renthal and centralizes the single-cell transcriptomic, epigenomic, and next-generation sequencing efforts with an experienced team in both the development, optimization, and analysis of single-cell and next-generation sequencing efforts. The centralization of these cutting-edge technologies will ensure that the established protocols can be applied systematically to all samples from the Center. Specifically, the Core will provide single-cell and single-nucleus multi-omic services to characterize pain-related tissues in both Projects 1 and 2. They will then provide both Projects rapid next-generation sequencing services with 1-3 day turnaround times at cost. Finally, the Core will remain at the cutting-edge of the rapidly evolving field of single-cell genomics and continue to bring innovations that improve quality and reduce costs back to the Center. The Core will also coordinate with similar Cores across the U19 PRECISION Human Pain Network to reconcile protocols and best practices.