Multi-Omics Core

多组学核心

基本信息

批准号：
10594340
负责人：
William Russell Renthal
金额：
$ 81.06万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-19 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10594340
关键词：
Back Benchmarking Biological Assay Cell Communication Cell Nucleus Cells Chromium Consultations Core Facility DNA Data Development Dissociation Drops Ecosystem Emergency Situation Ensure Freezing Fresh Tissue Gene Expression Genomics Genotype High-Throughput Nucleotide Sequencing Hour Human Individual Infrastructure Institutes Laboratories Learning Libraries Output Pain Pain Clinics Process Protocols documentation Quality Control RNA Reporting Research Research Personnel Resources Sampling Sensory Ganglia Services Technology Time Tissues Training XCL1 gene assay development cell type chronic pain cost cost effective cost effectiveness data standards epigenomics experience human tissue improved innovation member multi-ethnic multidisciplinary multiple omics next generation sequencing pain processing single cell analysis tool transcriptomics whole genome

项目摘要

PROJECT SUMMARY (Multi-omics Core) Single-cell transcriptomics is a central component of the PRECISION Human Pain Network because these powerful new tools provide unprecedented opportunities for understanding the cell types, molecules, and cell- cell interactions that contribute to chronic pain. The Multi-omics Core will be led by Dr. Renthal and centralizes the single-cell transcriptomic, epigenomic, and next-generation sequencing efforts with an experienced team in both the development, optimization, and analysis of single-cell and next-generation sequencing efforts. The centralization of these cutting-edge technologies will ensure that the established protocols can be applied systematically to all samples from the Center. Specifically, the Core will provide single-cell and single-nucleus multi-omic services to characterize pain-related tissues in both Projects 1 and 2. They will then provide both Projects rapid next-generation sequencing services with 1-3 day turnaround times at cost. Finally, the Core will remain at the cutting-edge of the rapidly evolving field of single-cell genomics and continue to bring innovations that improve quality and reduce costs back to the Center. The Core will also coordinate with similar Cores across the U19 PRECISION Human Pain Network to reconcile protocols and best practices.

项目摘要（多词核心）单细胞转录组学是精确人类疼痛网络的核心组成部分强大的新工具提供了前所未有的机会，可以理解细胞类型，分子和细胞 - 导致慢性疼痛的细胞相互作用。多派核心将由Renthal博士领导，并集中与经验丰富的团队一起进行的单细胞转录组，表观基因组和下一代测序工作单细胞和下一代测序工作的开发，优化和分析。这这些尖端技术的集中化将确保可以应用已建立的协议系统地到中心的所有样本。具体而言，核心将提供单细胞和单核在两个项目1和2中表征与疼痛相关的组织表征的多摩尼克服务。然后，它们将提供两者计划以1-3天的周转时间的价格计划快速下一代测序服务。最后，核心将留在单细胞基因组迅速发展的领域的前沿，并继续带来创新这提高了质量并将成本降低到中心。核心还将与跨越类似核心协调 U19精确的人痛网与协议和最佳实践调和。