PROTEOMICS CORE

蛋白质组学核心的中心目标是提供蛋白质组学专业知识和最先进的技术 可通过国家中心获取、挖掘和解释蛋白质组学数据的资源 为项目蛋白质组学研究L-11测试个别假设。每个单独的项目都将利用 蛋白质组学核心资源的特定目的和蛋白质组学图谱研究是至关重要的 每个单独项目的组件。蛋白质组学核心将与所有项目互动，以协助样本 蛋白质组学实验前和实验中的准备、质量控制以及分析和解释 数据。位于MCW的国家蛋白质组学研究中心已建立的基础设施 开发的优化工具非常适合于蛋白质组的定量分析和蛋白质组的表征 蛋白质磷酸化的变化。这些工具、资源和专业知识将推进这三个项目 有效并生成独特的数据集，突出麻醉剂复杂的潜在机制 预适应。 对于这个PPG，蛋白质组学核心将 1)建立和完善质谱分析的标准分析程序 细胞亚蛋白质组和蛋白质磷酸化的定量。 2)对由以下机构提供的线粒体和胞质样本进行质谱分析 L一号项目的调查人员。 3)利用同位素标记和串联质谱仪对蛋白质组的变化进行定量。 4)用质谱学方法分析蛋白质磷酸化的变化。 5)提供可靠、可重复、统一和及时的蛋白质组样本分析 项目。 6)维护数据库和自动化分析程序，用于蛋白质鉴定、量化、 和通径分析。 7)提供专业知识和设备，协助调查人员进行数据分析和解释。

The central objective of the Proteomics Core is to provide proteomics expertise and state-of-the-art resources for proteomics data acquisition, mining, and interpretation available through the National Center for Proteomics Research for Projects l-lll to test individual hypotheses. Each individual project will utilize the resources of the Proteomics Core in their specific aims, and the proteomic profiling studies are critical components of each individual Project. The Proteomics Core will interact with all projects to assist in sample preparation, quality control before and during proteomic experiments, and with the analysis and interpretation of data. The established infrastructure of the National Center for Proteomics Research at MCW has developed optimized tools ideally suited for the quantitative analysis of proteomes and the characterization of changes in protein phosphorylation. These tools, resources, and expertise will advance all three projects effectively and generate unique datasets highlighting the complex underlying mechanisms of anesthetic preconditioning. For this PPG, the Proteomics Core will 1) Establish and improve standard analytical procedures for mass spectrometric analysis and quantification of cellular sub-proteomes and protein phosphorylation. 2) Perform mass spectrometric analyses of mitochondrial and cytosolic samples provided by the investigators of Projects l-lll. 3) Quantify proteomic changes using isotopic labeling and tandem mass spectrometry. 4) Analyze changes in protein phosphorylation using mass spectrometric approaches. 5) Provide reliable, reproducible, uniform and timely analyses of proteomic samples for all three projects. 6) Maintain databases and automated analysis procedures for protein identification, quantification, and pathway analysis. 7) Provide expertise and equipment to assist investigators in data analysis and interpretation.