Bioreagents & Resources Core
生物试剂
基本信息
- 批准号:10713943
- 负责人:
- 金额:$ 18.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-20 至 2028-08-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAnimalsArchivesBarrett EsophagusBarrett&aposs carcinogenesisBasic ScienceBiological AssayBloodBlood specimenBody FluidsCell LineClinicalClinical ResearchCollaborationsCollectionColorComplexComprehensive Cancer CenterConfidentiality of Patient InformationConsultationsCustomDNADNA LibraryDevelopmentDiagnosticDissectionDysplasiaEPHB2 geneEnsureEpitheliumEsophageal AdenocarcinomaEsophageal NeoplasmsEsophageal TissueEsophageal injuryEsophagectomyEsophagusFacultyFamilyFamily suidaeFormalinFreezingFresh TissueFundingGenomicsGlandGoalsHigh grade dysplasiaHistologyHumanImmunofluorescence ImmunologicImmunohistochemistryIn Situ HybridizationInheritedInstitutional Review BoardsInvestigationKnock-inKnockout MiceLinkMedical RecordsMetaplasiaMicrodissectionModelingMolecularMorphologyMusOutcomeParaffin EmbeddingPathologistPathologyPathway interactionsPatientsPlayPredisposing FactorPredispositionPreparationProceduresProgram Research Project GrantsQuality ControlRNAReagentRecordsResearch PersonnelResearch Project GrantsResource SharingResourcesRoleSamplingScheduleServicesSignal TransductionSiteSpecimenSubmucosaSusceptibility GeneTechnologyTest ResultTissue EmbeddingTissue MicroarrayTissue ProcurementsTissue SampleTissuesTranslational ResearchUnited States National Institutes of HealthWorkbiobankblood fractionationcancer preventioncarcinogenesiscentral databasedesignesophageal carcinogenesisexperiencefollow-upgastrointestinalhuman tissueinjury and repairinsightlaser capture microdissectionmeetingsmouse modelnovelnovel strategiesprogramsquality assurancerepositoryresearch studyscreeningsingle cell sequencingskillssuccesstechnology/techniquetissue archivetissue processingtissue resourcetranscriptomicswound healing
项目摘要
PROJECT SUMMARY
The Biospecimen and Reagents Core [BRC] provides high quality annotated specimens and expert pathology
consultation to enhance the P01 projects. The BR&C CWRU and Duke components have long track records of
supporting multi-investigator basic, translational and clinical research relating to multiple aspects of the
Barrett's Esophagus [BE] and BE- neoplasia. The BRC is co-lead by two nationally recognized gastrointestinal
pathologists with expertise in esophageal pathology who have well established track records in tissue-based
translational research. The BRC co-leaders have worked together for many years and have longstanding
collaborations with the P01 teams. The BRC components have differencing and complementary resources.
Both the CWRU and the Duke components have large bioarchives which have been used to support multiple
large scale NIH funded projects for over 20+ years. The BR&C has tissue and blood biospecimens from over
6,200 patients suitable for this P01. The BR&C leverages the Case CCC Shared Resources, the UH Pathology
Translational Research Core and the Duke BioRepository & Precision Pathology Center shared resource to
maximize efficiencies, utilize their expertise and advanced technologies.
For Project 1 the BRC was integral to the discovery of germline abnormalities of VSIG10L as a predisposing
factor in some patients with Familial Barrett's Esophagus. The BRC worked with Prj 1 to define the knock in
and knock out mouse models which form the basis of this project and identified a multilayer epithelium complex
in Prj 1 murine models – recognized as a robust marker of BE changes and linked to BE in humans.
For Project 2 the BRC and collaborators developed the novel approach of using pigs as a model for
esophageal injury – including the study of esophageal submucosal glands [ESMGs] as a potential site of origin
of BE. Inspired by this effort, Prj 2 and the BRC identified and provided sections of ESMGs during screening of
multiple esophagectomies and then worked with Prj 2 to immunoprofile the ESMGs. The molecular
mechanisms identified formed the basis for the project.
For Project 3 the BRC supplied high quality tissue samples and immunohistochemistry expertise in the
investigation of EPHB2 signaling in esophageal carcinogenesis used to define potential pathways of
development of BE – results of which formed the basis for the project.
The BRC, with Core C, will continue to provide support to the P01 investigators and will enhance their projects
by supplying quality controlled esophagus tissues, human and animal pathology expertise and facilitate state of
the art tissue diagnostics including immunohistochemistry, ISH, tissue microdissection, spatial transcriptomics
and single cell sequencing.
项目摘要
生物标本和试剂核心[BRC]提供高质量的注释标本和专家病理学
加强P01项目的咨询。BR&C CWRU和杜克组件具有长期的跟踪记录,
支持多研究者基础,转化和临床研究,涉及的多个方面,
Barrett食管[BE]和BE-瘤形成。BRC由两个国家认可的胃肠
具有食管病理学专业知识的病理学家,他们在基于组织的
翻译研究布拉迪斯拉发区域中心共同领导人已经合作多年,
与P01团队合作。布拉迪斯拉发区域中心各组成部分拥有不同和互补的资源。
CWRU和杜克组件都有大量的生物档案,用于支持多个
美国国立卫生研究院资助的大型项目超过20年。BR&C有超过1000名
6,200例患者适合本P01。BR&C利用案例CCC共享资源,UH病理学
转化研究中心和杜克生物库和精密病理学中心共享资源,
最大限度地提高效率,利用他们的专业知识和先进技术。
对于项目1,BRC对于发现VSIG 10 L的生殖系异常是不可或缺的,
家族性Barrett食管患者中的某些因素。BRC与Prj 1一起定义了敲入
并敲除小鼠模型,这些模型构成了本项目的基础,并确定了多层上皮复合体,
在Prj 1鼠模型中-被认为是BE变化的稳健标志物,并与人类BE相关。
对于项目2,BRC和合作者开发了一种新的方法,使用猪作为模型,
食管损伤-包括食管粘膜下腺体[ESMG]作为潜在起源部位的研究
的BE。在这一努力的启发下,项目2和布拉迪斯拉发区域中心在筛选
多次食管切除术,然后与Prj 2一起进行ESMG免疫分析。分子
所确定的机制构成了该项目的基础。
对于项目3,BRC提供了高质量的组织样本和免疫组织化学专业知识,
食管癌发生中EPHB 2信号通路的研究,用于确定
BE的发展-其结果构成了该项目的基础。
布拉迪斯拉发区域中心与核心C将继续向P01调查员提供支持,并将加强他们的项目
通过提供质量受控的食管组织,人类和动物病理学专业知识,
现有技术组织诊断学包括免疫组织化学、ISH、组织显微切割、空间转录组学
和单细胞测序。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JOSEPH EDWARD WILLIS其他文献
JOSEPH EDWARD WILLIS的其他文献
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{{ truncateString('JOSEPH EDWARD WILLIS', 18)}}的其他基金
Validation of Methylated Vimentin as a Diagnostic Test for Barrett's Esophagus
甲基化波形蛋白作为巴雷特食管诊断测试的验证
- 批准号:
9923576 - 财政年份:2016
- 资助金额:
$ 18.6万 - 项目类别:
Project 2: Translational Significance of a Mutational Signature of African American Colon Cancers
项目 2:非裔美国人结肠癌突变特征的转化意义
- 批准号:
10227753 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
Identification of Significant Race Associated Colon Cancer Drive Gene Mutations
显着种族相关结肠癌驱动基因突变的鉴定
- 批准号:
8097073 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
Patients Registry -Virtual Biorepository Core
患者登记处-虚拟生物样本库核心
- 批准号:
10153705 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
Identification of Significant Race Associated Colon Cancer Drive Gene Mutations
显着种族相关结肠癌驱动基因突变的鉴定
- 批准号:
8330850 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
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