Bioreagents & Resources Core
生物试剂
基本信息
- 批准号:10713943
- 负责人:
- 金额:$ 18.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-20 至 2028-08-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAnimalsArchivesBarrett EsophagusBarrett&aposs carcinogenesisBasic ScienceBiological AssayBloodBlood specimenBody FluidsCell LineClinicalClinical ResearchCollaborationsCollectionColorComplexComprehensive Cancer CenterConfidentiality of Patient InformationConsultationsCustomDNADNA LibraryDevelopmentDiagnosticDissectionDysplasiaEPHB2 geneEnsureEpitheliumEsophageal AdenocarcinomaEsophageal NeoplasmsEsophageal TissueEsophageal injuryEsophagectomyEsophagusFacultyFamilyFamily suidaeFormalinFreezingFresh TissueFundingGenomicsGlandGoalsHigh grade dysplasiaHistologyHumanImmunofluorescence ImmunologicImmunohistochemistryIn Situ HybridizationInheritedInstitutional Review BoardsInvestigationKnock-inKnockout MiceLinkMedical RecordsMetaplasiaMicrodissectionModelingMolecularMorphologyMusOutcomeParaffin EmbeddingPathologistPathologyPathway interactionsPatientsPlayPredisposing FactorPredispositionPreparationProceduresProgram Research Project GrantsQuality ControlRNAReagentRecordsResearch PersonnelResearch Project GrantsResource SharingResourcesRoleSamplingScheduleServicesSignal TransductionSiteSpecimenSubmucosaSusceptibility GeneTechnologyTest ResultTissue EmbeddingTissue MicroarrayTissue ProcurementsTissue SampleTissuesTranslational ResearchUnited States National Institutes of HealthWorkbiobankblood fractionationcancer preventioncarcinogenesiscentral databasedesignesophageal carcinogenesisexperiencefollow-upgastrointestinalhuman tissueinjury and repairinsightlaser capture microdissectionmeetingsmouse modelnovelnovel strategiesprogramsquality assurancerepositoryresearch studyscreeningsingle cell sequencingskillssuccesstechnology/techniquetissue archivetissue processingtissue resourcetranscriptomicswound healing
项目摘要
PROJECT SUMMARY
The Biospecimen and Reagents Core [BRC] provides high quality annotated specimens and expert pathology
consultation to enhance the P01 projects. The BR&C CWRU and Duke components have long track records of
supporting multi-investigator basic, translational and clinical research relating to multiple aspects of the
Barrett's Esophagus [BE] and BE- neoplasia. The BRC is co-lead by two nationally recognized gastrointestinal
pathologists with expertise in esophageal pathology who have well established track records in tissue-based
translational research. The BRC co-leaders have worked together for many years and have longstanding
collaborations with the P01 teams. The BRC components have differencing and complementary resources.
Both the CWRU and the Duke components have large bioarchives which have been used to support multiple
large scale NIH funded projects for over 20+ years. The BR&C has tissue and blood biospecimens from over
6,200 patients suitable for this P01. The BR&C leverages the Case CCC Shared Resources, the UH Pathology
Translational Research Core and the Duke BioRepository & Precision Pathology Center shared resource to
maximize efficiencies, utilize their expertise and advanced technologies.
For Project 1 the BRC was integral to the discovery of germline abnormalities of VSIG10L as a predisposing
factor in some patients with Familial Barrett's Esophagus. The BRC worked with Prj 1 to define the knock in
and knock out mouse models which form the basis of this project and identified a multilayer epithelium complex
in Prj 1 murine models – recognized as a robust marker of BE changes and linked to BE in humans.
For Project 2 the BRC and collaborators developed the novel approach of using pigs as a model for
esophageal injury – including the study of esophageal submucosal glands [ESMGs] as a potential site of origin
of BE. Inspired by this effort, Prj 2 and the BRC identified and provided sections of ESMGs during screening of
multiple esophagectomies and then worked with Prj 2 to immunoprofile the ESMGs. The molecular
mechanisms identified formed the basis for the project.
For Project 3 the BRC supplied high quality tissue samples and immunohistochemistry expertise in the
investigation of EPHB2 signaling in esophageal carcinogenesis used to define potential pathways of
development of BE – results of which formed the basis for the project.
The BRC, with Core C, will continue to provide support to the P01 investigators and will enhance their projects
by supplying quality controlled esophagus tissues, human and animal pathology expertise and facilitate state of
the art tissue diagnostics including immunohistochemistry, ISH, tissue microdissection, spatial transcriptomics
and single cell sequencing.
项目总结
生物样品和试剂核心[BRC]提供高质量的带注释的标本和专家病理学
咨询以加强P01项目。BR&C CWRU和Duke组件拥有长期的记录
支持多名研究人员进行基础、转化和临床研究,这些研究涉及
Barrett‘s食道和BE瘤。BRC由两个国家公认的胃肠疾病患者共同领导
在食道病理学方面有专业知识的病理学家,在组织病理学方面有良好的跟踪记录
翻译研究。BRC联合领导人多年来一直在一起工作,长期
与P01团队的合作。预算资源中心各组成部分具有差异性和互补性资源。
CWRU和Duke组件都有大型生物档案,用于支持多个
美国国立卫生研究院资助的大型项目超过20年。BR&C的组织和血液中有来自
6,200名患者适用于此P01。BR&C利用Case CCC共享资源,UH病理
翻译研究核心和杜克生物信息库和精密病理中心共享资源以
最大限度地提高效率,利用他们的专业知识和先进技术。
在项目1中,BRC是发现VSIG10L胚系异常作为易感基因不可或缺的一部分
一些家族性Barrett‘s食道患者的因素。BRC与PRJ 1合作定义了敲击
并剔除了构成该项目基础的小鼠模型,并确定了一种多层上皮复合体
在Prj1小鼠模型中-被认为是BE变化的一个强有力的标记,并与人类的BE有关。
在项目2中,BRC和合作者开发了使用猪作为模型的新方法
食道损伤--包括将食道粘膜下腺[ESMGs]作为潜在起源部位的研究
当然是。在这一努力的启发下,PRJ 2和BRC在筛选期间确定并提供了ESMGs的部分
多次食道切除,然后与Prj2一起工作,以免疫分析ESMGs。分子
确定的机制构成了该项目的基础。
对于项目3,BRC提供了高质量的组织样本和免疫组织化学专业知识
食管癌发生过程中EPHB2信号转导途径的研究
其成果构成了该项目的基础。
BRC和核心C将继续为P01调查人员提供支持,并将加强他们的项目
通过提供质量受控的食道组织,人类和动物的病理专业知识,并促进状态
ART组织诊断学包括免疫组织化学、原位杂交、组织显微解剖、空间转录学
和单细胞测序。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOSEPH EDWARD WILLIS其他文献
JOSEPH EDWARD WILLIS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOSEPH EDWARD WILLIS', 18)}}的其他基金
Validation of Methylated Vimentin as a Diagnostic Test for Barrett's Esophagus
甲基化波形蛋白作为巴雷特食管诊断测试的验证
- 批准号:
9923576 - 财政年份:2016
- 资助金额:
$ 18.6万 - 项目类别:
Project 2: Translational Significance of a Mutational Signature of African American Colon Cancers
项目 2:非裔美国人结肠癌突变特征的转化意义
- 批准号:
10227753 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
Identification of Significant Race Associated Colon Cancer Drive Gene Mutations
显着种族相关结肠癌驱动基因突变的鉴定
- 批准号:
8097073 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
Patients Registry -Virtual Biorepository Core
患者登记处-虚拟生物样本库核心
- 批准号:
10153705 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
Identification of Significant Race Associated Colon Cancer Drive Gene Mutations
显着种族相关结肠癌驱动基因突变的鉴定
- 批准号:
8330850 - 财政年份:2011
- 资助金额:
$ 18.6万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 18.6万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 18.6万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 18.6万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 18.6万 - 项目类别:
Discovery Early Career Researcher Award
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 18.6万 - 项目类别:
Continuing Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 18.6万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 18.6万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Analysis of thermoregulatory mechanisms by the CNS using model animals of female-dominant infectious hypothermia
使用雌性传染性低体温模型动物分析中枢神经系统的体温调节机制
- 批准号:
23KK0126 - 财政年份:2023
- 资助金额:
$ 18.6万 - 项目类别:
Fund for the Promotion of Joint International Research (International Collaborative Research)
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 18.6万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 18.6万 - 项目类别:
Training Grant














{{item.name}}会员




