Bioreagents & Resources Core

生物试剂

• 批准号: 10713943
• 负责人: JOSEPH EDWARD WILLIS
• 金额: $ 18.6万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-20 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10713943
• 关键词: Address; Animals; Archives; Barrett Esophagus; Barrett' s carcinogenesis; Basic Science; Biological Assay; Blood; Blood specimen; Body Fluids; Cell Line; Clinical; Clinical Research; Collaborations; Collection; Color; Complex; Comprehensive Cancer Center; Confidentiality of Patient Information; Consultations; Custom; DNA; DNA Library; Development; Diagnostic; Dissection; Dysplasia; EPHB2 gene; Ensure; Epithelium; Esophageal Adenocarcinoma; Esophageal Neoplasms; Esophageal Tissue; Esophageal injury; Esophagectomy; Esophagus; Faculty; Family; Family suidae; Formalin; Freezing; Fresh Tissue; Funding; Genomics; Gland; Goals; High grade dysplasia; Histology; Human; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Inherited; Institutional Review Boards; Investigation; Knock-in; Knockout Mice; Link; Medical Records; Metaplasia; Microdissection; Modeling; Molecular; Morphology; Mus; Outcome; Paraffin Embedding; Pathologist; Pathology; Pathway interactions; Patients; Play; Predisposing Factor; Predisposition; Preparation; Procedures; Program Research Project Grants; Quality Control; RNA; Reagent; Records; Research Personnel; Research Project Grants; Resource Sharing; Resources; Role; Sampling; Schedule; Services; Signal Transduction; Site; Specimen; Submucosa; Susceptibility Gene; Technology; Test Result; Tissue Embedding; Tissue Microarray; Tissue Procurements; Tissue Sample; Tissues; Translational Research; United States National Institutes of Health; Work; biobank; blood fractionation; cancer prevention; carcinogenesis; central database; design; esophageal carcinogenesis; experience; follow-up; gastrointestinal; human tissue; injury and repair; insight; laser capture microdissection; meetings; mouse model; novel; novel strategies; programs; quality assurance; repository; research study; screening; single cell sequencing; skills; success; technology/technique; tissue archive; tissue processing; tissue resource; transcriptomics; wound healing

PROJECT SUMMARY The Biospecimen and Reagents Core [BRC] provides high quality annotated specimens and expert pathology consultation to enhance the P01 projects. The BR&C CWRU and Duke components have long track records of supporting multi-investigator basic, translational and clinical research relating to multiple aspects of the Barrett's Esophagus [BE] and BE- neoplasia. The BRC is co-lead by two nationally recognized gastrointestinal pathologists with expertise in esophageal pathology who have well established track records in tissue-based translational research. The BRC co-leaders have worked together for many years and have longstanding collaborations with the P01 teams. The BRC components have differencing and complementary resources. Both the CWRU and the Duke components have large bioarchives which have been used to support multiple large scale NIH funded projects for over 20+ years. The BR&C has tissue and blood biospecimens from over 6,200 patients suitable for this P01. The BR&C leverages the Case CCC Shared Resources, the UH Pathology Translational Research Core and the Duke BioRepository & Precision Pathology Center shared resource to maximize efficiencies, utilize their expertise and advanced technologies. For Project 1 the BRC was integral to the discovery of germline abnormalities of VSIG10L as a predisposing factor in some patients with Familial Barrett's Esophagus. The BRC worked with Prj 1 to define the knock in and knock out mouse models which form the basis of this project and identified a multilayer epithelium complex in Prj 1 murine models – recognized as a robust marker of BE changes and linked to BE in humans. For Project 2 the BRC and collaborators developed the novel approach of using pigs as a model for esophageal injury – including the study of esophageal submucosal glands [ESMGs] as a potential site of origin of BE. Inspired by this effort, Prj 2 and the BRC identified and provided sections of ESMGs during screening of multiple esophagectomies and then worked with Prj 2 to immunoprofile the ESMGs. The molecular mechanisms identified formed the basis for the project. For Project 3 the BRC supplied high quality tissue samples and immunohistochemistry expertise in the investigation of EPHB2 signaling in esophageal carcinogenesis used to define potential pathways of development of BE – results of which formed the basis for the project. The BRC, with Core C, will continue to provide support to the P01 investigators and will enhance their projects by supplying quality controlled esophagus tissues, human and animal pathology expertise and facilitate state of the art tissue diagnostics including immunohistochemistry, ISH, tissue microdissection, spatial transcriptomics and single cell sequencing.

项目总结