Macaque Resources

猕猴资源

• 批准号: 7663388
• 负责人: Louis J. Picker
• 金额: --
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-16 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7663388
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Animals; Antibodies; Antigens; Arts; Biological Assay; Blood; Budgets; CD8B1 gene; Cells; Cellular Immunology; Complex; Disease; Ensure; Flow Cytometry; Goals; HIV; HIV vaccine; Human; Human Resources; Immune response; Immune system; Immunity; Immunologic Monitoring; Infection prevention; Instruction; Investigation; Macaca; Macaca mulatta; Modeling; Oregon; Primates; Protocols documentation; Research; Research Design; Research Infrastructure; Research Personnel; Resources; SIV; Sampling; Structure; T-Lymphocyte; Technical Expertise; Tissues; Vaccination; Vaccine Research; Vaccines; Virus Diseases; animal care; cytokine; experience; immunogenicity; meetings; neutralizing antibody; nonhuman primate; pandemic disease; pre-clinical; programs; simian human immunodeficiency virus; vaccine development

The Indian rhesus macaque develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Because of the similarity of the immune systems of macaques and humans, preclinical vaccine development is heavily dependent on the SIV and SHIV macaque models. Their research value notwithstanding, nonhuman primates (NHPs) are complex, higher order species that require specialized infrastructure and expertise in a research setting. The NHP Core seeks to consolidate the experimental animal and cellular immunology portions of the HIV Vaccine Research and Design (HIVRAD) Program project, "Programming HIV Immune Response for Broadly Neutralizing Antibodies by Vaccination" into a single Core composed of highly experienced professional and technical personnel and state-of-the-art resources. The NHP Core, functioning within the Oregon National Primate Research Center (ONPRC), will provide infrastructure and professional and technical expertise for the NHP experimental protocol and the antigen (Ag)-specific CD4+ and CD8+ T cell analyses supporting the "HIV Quasispecies Vaccine Immunogenicity and SHIV Challenge Study". The Specific Aims are: 1) to provide specialized expertise and infrastructure for comprehensive, efficient and safe conduct of the nonhuman primate-related portions of the HIVRAD Program including animal selection, research protocol implementation and execution, sample acquisition and distribution, animal care, and project budget administration, and 2) to provide the HIVRAD Program with flow cytometric assays of Ag- specific T cell immunity and SHIV immunopathogenesis. The immune monitoring section of the Nonhuman Primate Core will provide sophisticated assays of SIV/HIV-specific T cell immunity (polychromatic cytokine flow cytometry) and SHIV immunopathogenesis (polychromatic phenotypic analysis) of SHIV target cell dynamics in blood and tissue. RELEVANCE (See instructions): A safe and effective vaccine remains the best hope of controlling the human immunodeficiency virus (HIV) pandemic. The studies in this proposal focus on developing vaccine strategies to promote broadly neutralizing anti-HIV antibody, a key component of the host immune response against HIV that may prevent infection.

印度恒河猴患上一种与人类获得性免疫缺陷极为相似的疾病 当被猴免疫缺陷病毒（SIV）或嵌合猴-人感染时， 免疫缺陷病毒（SHIV），并代表了最好的动物模型的HIV感染。因为 由于猕猴和人类免疫系统的相似性，临床前疫苗的开发在很大程度上 依赖于SIV和SHIV猕猴模型。尽管它们有研究价值， 灵长类动物（NHP）是复杂的高阶物种，需要专门的基础设施和专业知识， 研究设置。NHP核心旨在巩固实验动物和细胞免疫学 艾滋病毒疫苗研究与设计（HIVRAD）计划项目“艾滋病毒免疫程序设计“的部分内容 通过疫苗接种对广泛中和抗体的反应”的核心， 经验丰富的专业技术人员和最先进的资源。NHP核心，运行 在俄勒冈州国家灵长类动物研究中心（ONPRC），将提供基础设施和专业 NHP实验方案和抗原（Ag）特异性CD 4+和CD 8 + T细胞 支持“HIV准种疫苗免疫原性和SHIV攻毒研究”的细胞分析。的 具体目标是：1）提供专业知识和基础设施， 开展HIVRAD计划中与非人灵长类动物相关的部分，包括动物选择， 研究方案的实施和执行，样本采集和分发，动物护理，以及 项目预算管理，以及2）为HIVRAD计划提供流式细胞术检测Ag- 特异性T细胞免疫和SHIV免疫发病机制。非人免疫监测部分 Primate Core将提供SIV/HIV特异性T细胞免疫（多色细胞因子）的复杂检测 流式细胞术）和SHIV免疫发病机制（多色表型分析）的SHIV靶细胞 血液和组织中的动力学。 相关性（参见说明）： 一种安全有效的疫苗仍然是控制人类免疫缺陷病毒（艾滋病毒）的最大希望 流行病本提案中的研究重点是制定疫苗战略， 中和性抗HIV抗体是宿主对HIV免疫反应的关键成分， 感染