Studies of Yeast BTN1P and Human CLN3P in Yeast

酵母中酵母BTN1P和人CLN3P的研究

• 批准号: 7564094
• 负责人: DAVID A. PEARCE
• 金额: $ 12.71万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-28 至 2009-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7564094
• 关键词: Adolescent; Amino Acids; Arginine; Biochemical; Biochemistry; Biological Assay; Biology; CLN3 gene; Cell physiology; Cells; Child; Codon Nucleotides; Collection; Complement; Coupled; Coupling; Data; Defect; Disease; Electrophoresis; Equilibrium; Functional disorder; Gene Expression; Gene Proteins; Genes; Genetic; Genetic Screening; Genotype; Goals; Grant; Growth; Homologous Gene; Human; Incidence; Individual; Lead; Live Birth; MALDI-TOF Mass Spectrometry; Mammalian Cell; Measures; Mediating; Membrane; Molecular; Mutation; Neurodegenerative Disorders; Neuronal Ceroid-Lipofuscinosis; Oligonucleotide Microarrays; Organism; Pathogenesis; Pathway interactions; Phase; Phenotype; Phosphorylation Site; Polyamines; Polyphosphates; Principal Investigator; Process; Proteins; Proton Pump; Protons; Regulation; Reporting; Research; Role; Screening procedure; Sorting - Cell Movement; Spielmeyer-Vogt Disease; Staging; Translations; Trehalose; Vacuole; Variant; Work; Yeast Model System; Yeasts; base; disease-causing mutation; enzyme activity; extracellular; glycosylation; overexpression; pH Homeostasis; prenylation; programs; protein transport; small molecule; sugar; trafficking; vacuolar H+-ATPase

DESCRIPTION (provided by applicant): The neuronal ceroid-lipofuscinoses (NCL) are possibly the most common group of progressive neurodegenerative diseases in children, with an incidence as high as one in 12,500 live births, and with about 440,000 carriers in the USA. Juvenile NCL/Batten disease is the most common of these disorders and the subject of this proposal. Individuals with the disease were found to harbor a 1 kb deletion, which introduces a frameshift that leads to a predicted translation product of 181 amino acids, of which only the first 153 residues correspond to the first 153 of the normal 438 amino acid CLN3 gene product. The yeast homolog to CLN3 was identified and designated BTN1. We had previously shown that Btn1 p may be involved in maintaining pH homeostasis. Importantly, CLN3 is able to complement the alteration in vacuolar pH in the yeast model lacking Btn1 p, indicating that they have similar, if not the same cellular functions. Our more recent studies indicated that lacking Btnlp resulted in a defect in vacuolar transport of arginine, and again CLN3 is able to complement the defect in arginine transport. Overall our studies indicate that yeast cells work to maintain pH homeostasis, and that Btn1 p is an integral part of the biology of this process. This proposal sets out to investigate bfn1-/l-mediated disruption of pH homeostasis within the single cell of yeast. By elucidating the mechanisms by which this single celled organism balances intracellular pH and by uncovering the specific function of Btnlp and other proteins in the BTN-pathway we will establish a basis for understanding pH homeostasis in mammalian cells. We propose to further characterize the biochemistry of Btn1 p-dependent regulation of vacuolar pH. Moreover by exploiting assays that correlate to Btn1 p function such as vacuolar arginine transport and vacuolar proton pumping we will further define the structural requirements of Btn1p/CLN3. Concomitant to these studies we will identify components of the BTN1- pathway through use of a variety of genetic screens such as phenotypic suppression and synthetic lethality. Finally we will characterize the pathway of trafficking Btnlp to the vacuole. Further understanding of Btnlp (and ClnSp) in yeast will provide valuable information on the pathogenesis of Batten disease.

描述（由申请人提供）：神经元蜡样脂褐质病（NCL）可能是儿童中最常见的一组进行性神经退行性疾病，其发病率高达1/12，500活产，在美国约有440，000携带者。青少年NCL/Batten病是这些疾病中最常见的，也是本提案的主题。发现患有该疾病的个体具有1 kb缺失，其引入移码，导致预测的181个氨基酸的翻译产物，其中仅前153个残基对应于正常的438个氨基酸CLN 3基因产物的前153个。CLN 3的酵母同源物被鉴定并命名为BTN 1。我们先前已经表明，Btn 1 p可能参与维持pH稳态。重要的是，CLN 3能够补充缺乏Btn 1 p的酵母模型中液泡pH的改变，表明它们具有相似的细胞功能。我们最近的研究表明，缺乏Btnlp导致精氨酸的液泡转运缺陷，并且CLN 3再次能够补充精氨酸转运的缺陷。总的来说，我们的研究表明，酵母细胞的工作，以保持pH值的稳态，Btn 1 p是生物学的这一过程中不可或缺的一部分。该提议旨在研究bfn 1-/l介导的酵母单细胞内pH稳态的破坏。通过阐明这种单细胞生物平衡细胞内pH的机制，并通过揭示Btnlp和其他蛋白质在BTN途径中的特定功能，我们将为理解哺乳动物细胞中的pH稳态奠定基础。我们建议进一步表征Btn 1 p依赖的液泡pH调节的生物化学。此外，通过利用与Btn 1 p功能相关的测定，如液泡精氨酸转运和液泡质子泵，我们将进一步定义Btn 1 p/CLN 3的结构要求。伴随着这些研究，我们将通过使用各种遗传筛选，如表型抑制和合成致死率，确定BTN 1途径的组成部分。最后，我们将描述Btnlp运输到液泡的途径。进一步了解Btnlp（和ClnSp）在酵母中的作用将为Batten病的发病机制提供有价值的信息。