Molecular Mechanisms Underlying Adaptor-Mediated Integrin Signaling in a Species-Specific Manner

项目摘要 这是一份NIGMS行政补充申请，用于购买NIGMS赠款R35 GM 119560， 来自Wyatt Technology的miniDAWN多角度光散射（MALS）检测器。我们以前的结构和 对整合素信号通路的功能研究表明，整合素活性受到一些信号通路的严格调节， 衔接蛋白蛋白质复合物或蛋白质同源寡聚体的形成介导了信号转导 导致整合素活化和聚集。有了miniDAWN MALS探测器，我们可以监测地层 这些复合物/寡聚体的精确测定蛋白质的绝对摩尔质量和大小 溶液中的大分子。该工具将提供必要的能力，以最大限度地提高生产力， 充分实现拟议研究项目的目标。机构配套资金将有助于 购买此工具。

Project Summary This is a request for an NIGMS Administrative Supplement to NIGMS grant R35GM119560, to purchase a miniDAWN Multi-Angle Light Scattering (MALS) Detector from Wyatt Technology. Our previous structural and functional studies of integrin signaling pathway reveal that integrin activity is tightly regulated by a few signaling adaptor proteins. Formation of protein complexes or protein homo-oligomers mediates the signal transduction that leads to integrin activation and clustering. With the miniDAWN MALS detector, we can monitor the formation of these complexes/oligomers by accurately determining the absolute molar masses and sizes of protein macromolecules in solution. The instrument will provide the necessary capabilities to maximize productivity and fully achieve the aims of the proposed research project. Institutional matching funds will contribute to the purchase of this instrument.