Molecular Mechanisms Underlying Adaptor-Mediated Integrin Signaling in a Species-Specific Manner

项目摘要 这是对NIGMS授权的NIGMS行政补充的请求R35GM119560，以购买 怀亚特科技的mini DAWN多角光散射(MALS)探测器。我们以前的结构和 整合素信号通路的功能研究表明，整合素活性受少数几个信号的严格调控 接头蛋白。蛋白质复合体或蛋白质低聚物的形成介导了信号转导 这会导致整合素的激活和聚集。使用微型DAWN MALS探测器，我们可以监控队形 通过准确确定蛋白质的绝对摩尔质量和大小来确定这些络合物/低聚物 溶液中的大分子。该工具将提供必要的能力，以最大限度地提高生产率和 完全达到了拟议研究项目的目标。机构配对基金将为 购买这种工具。

Project Summary This is a request for an NIGMS Administrative Supplement to NIGMS grant R35GM119560, to purchase a miniDAWN Multi-Angle Light Scattering (MALS) Detector from Wyatt Technology. Our previous structural and functional studies of integrin signaling pathway reveal that integrin activity is tightly regulated by a few signaling adaptor proteins. Formation of protein complexes or protein homo-oligomers mediates the signal transduction that leads to integrin activation and clustering. With the miniDAWN MALS detector, we can monitor the formation of these complexes/oligomers by accurately determining the absolute molar masses and sizes of protein macromolecules in solution. The instrument will provide the necessary capabilities to maximize productivity and fully achieve the aims of the proposed research project. Institutional matching funds will contribute to the purchase of this instrument.