Establish and Characterize an Air Liquid Interface In Vitro Exposure System

体外暴露系统气液界面的建立和表征

• 批准号: 10788077
• 负责人: Barney Sparrow
• 金额: $ 149万
• 依托单位: BATTELLE MEMORIAL INSTITU
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2023-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10788077
• 关键词: Aerosols; Air; Air Pollutants; Animals; Cells; Complex Mixtures; Data; Data Correlations; Dryness; Evaluation; Fiber; Goals; Government; Health Hazards; Human; In Vitro; Industrialization; Inhalation; Inhalation Exposure; Liquid substance; Lung; Population; Powder dose form; Rattus; System; Testing; Tissues; Toxicity Tests; Toxicology; design; exposure route; in vitro testing; in vivo; pilot test; programs; toxicant; vapor

The Division of Translational Toxicology (DTT) continued to optimize use of the government owned Vitrocell 48 2.0 plus exposure system to provide the program with in vitro capabilities that mimic inhalation exposure. The inhalation route of exposure is commonly seen among air pollutants as well as with industrial settings, and until recently has been very difficult to simulate in vitro. This system allows in vitro testing of potential toxicants for inhalation exposure which contributes to the program's goals of reducing the use of animals. This system provides high throughput and can be used for airborne substances such as vapors, complex mixtures of liquid aerosol, dry powders, and fibers. DTT is conducting additional evaluation of the system to increase confidence that the system will function as intended. This will be achieved through the design and conduct of system characterization and pilot in vitro toxicity testing of selected test agents with robust in vivo data for correlation, using tissue from representative human populations. The system also allows testing of cells from humans and rats to provide data supporting species extrapolation. Keyword: in vitro, inhalation exposure

翻译毒理学部门(DTT)继续优化使用政府拥有的Vitrocell 48 2.0 plus暴露系统，为该计划提供模拟吸入暴露的体外能力。暴露在空气污染物和工业环境中的吸入途径是常见的，直到最近还很难在体外模拟。该系统允许对吸入暴露的潜在毒物进行体外测试，这有助于实现该计划减少使用动物的目标。该系统提供高通量，可用于空气中的物质，如蒸气、液体气雾剂、干粉和纤维的复杂混合物。DTT正在对该系统进行额外的评估，以增加对该系统将按预期运行的信心。这将通过设计和进行系统表征和试验性体外毒性测试来实现，选定的测试剂具有可靠的活体数据用于相关，使用来自代表性人类群体的组织。该系统还允许测试来自人类和大鼠的细胞，以提供支持物种外推的数据。 关键词：体外，吸入暴露