Resource for Integrated Glycotechnology

整合糖类技术资源的首要目标是多学科的发展 糖生物学中问题的解决途径。特定焦点是与以下内容相关的问题的子集 糖胺多聚糖起作用。肝素、硫酸肝素和硫酸软骨素等糖胺多聚糖 在调节细胞内信号转导中起重要作用，影响免疫细胞向 感染，控制肿瘤的血管生成，调节神经元的再生。它们还可用作 病原体的受体。为了完成这些角色，结合蛋白与特定的区域相互作用 这些糖胺聚糖。了解这些相互作用是进行干预的重要一步。 人类疾病，然而关于识别的特定序列的信息很少，结构 相互作用的各个方面，或它们相互作用导致细胞反应的方式。缺乏 信息在一定程度上是由于这些碳水化合物的序列非常复杂 聚合物。该资源开发了通过结合以下方面的进展来提供此信息的技术 具有结合专一性的糖胺聚糖低聚物的分离与合成 核磁共振中基于光谱(MS)识别齐聚物结构的方法 基于光谱和质谱学的确定络合物三维结构的方法，在 糖胺聚糖-蛋白质相互作用的计算模型和预测，以及在细胞和 以生物化学为基础的监测生物反应的手段。推动技术发展的因素是 精选推动与外部合作者合作的生物医学项目。其中包括解决 Robo-Sit信号在血管生成中的作用以及DLB域在疟疾存活中的作用 胎盘感染中寄生虫与糖胺聚糖结合抗体检测细胞的特异性 异常，以及趋化因子对免疫细胞迁移的调节。技术被传播 通过广泛的培训计划和额外的协作以及由 资源。

The primary objective ofthe Resource for Integrated Glycotechnology is the development of multidisciplinary approaches to the solution of problems in glycobiology. A particular focus is a subset of problems related to glycosaminoglycan function. Glycosaminoglycans such as heparin, heparan sulfate, and chondroitin sulfate play important roles in modulating intracellular signaling, influencing the migration of immune cells to sites of infection, controlling angiogenesis in tumors, and regulating regeneration of neurons. They also serve as receptors for pathogenic organisms. To fulfill these roles, binding proteins interact with specific regions of these glycosaminoglycans. Understanding these interactions is an important step toward intervention in human disease, yet little information is available on the specific sequences recognized, the structural aspects of the interactions, or they way in which interactions result in cellular response. The lack of information is in part due to the extraordinary complexity of the sequences of these carbohydrate based polymers. The Resource develops technology to provide this information by combining advances in separation and synthesis of glycosaminoglycan oligomers that display binding specificity, in mass spectrometry (MS) based means of identifying oligomer structures, in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry based means of defining three dimensional structures of complexes, in computational modeling and prediction of glycosaminoglycan-protein interactions, and in cell and biochemically based means of monitoring a biological response. The technology development is driven by selected driving biomedical projects with external collaborators. These include ones that address the function of Robo-Slit signaling in angiogenesis, the function of DLB domains in survival of the malaria parasite in placental infection, the specificity of glycosaminoglycan binding antibodies in detection of cellular abnormalities, and the regulation of immune cell migration by chemokines. Technology is disseminated through extensive training programs and additional collaborations and service functions hosted by the Resource.