Upgrade for a 600 MHz Structural Biology NMR

升级为 600 MHz 结构生物学 NMR

• 批准号: 9075568
• 负责人: JAMES H. PRESTEGARD
• 金额: $ 59.99万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075568
• 关键词: 17 year old; Adopted; Area; Carbon; Communities; Complex; Detection; Development; Faculty; Future; Grant; Heat shock proteins; Immune response; Metabolism; Minor; NMR Spectroscopy; Natural Products; Neoplasm Metastasis; Performance; Polysaccharides; Post-Translational Protein Processing; Proteins; Protocols documentation; Protons; Regulation; Research; Role; Scientist; Signal Transduction; Site; Structure; System; United States National Institutes of Health; Universities; Virulence Factors; Work; base; digital; drug efficacy; improved; instrument; instrumentation; pathogenic bacteria; programs; protein complex; research study; response; stress protein; structural biology

 DESCRIPTION (provided by applicant): An upgrade of an existing 600 MHz NMR spectrometer used primarily for structural biology applications is proposed. The upgrade will replace a 17 year old Varian INOVA console with a new Bruker Avance III HD 600 High Performance Digital NMR Console and an existing cryobay with a Bruker cryoplatform. The upgrade will allow the use of a high sensitivity cryoprobe that is optimized for 13C (and 15N) detection along with 1H and 2H decoupling. The instrument is being placed in a well-staffed and supported NMR facility that serves the University of Georgia (UGA) scientific community, as well as a number of additional scientists throughout the region. The ability to observe 13C directly with high sensitivity will add to the facility a capability that is unique, and one that is becomin increasingly important in the characterization of biomolecular systems. These systems include large proteins or proteins that are parts of large complexes, proteins with intrinsically disordere regions, and natural products (including some glycans) that have significant numbers of non- protonated carbon sites. In addition to this unique capability, the system will execute, with sensitivity comparable or greater than non-cryo systems, the traditional proton detected triple resonance experiments that have become so important to structure determination of biomedically important proteins. In the latter case the more reliable and up-to-date system will add important capacity to serve the growing number of NMR users who pursue NMR-based structural work at UGA. Seven major users and three minor users have provided research descriptions as a part of this proposal. Their projects impact on areas such as: the role of glycans in development and metastasis, protein modifications in response to environmental stress, protein interactions in cell signaling and immune response, regulation of virulence factors in pathogenic bacteria, and improving drug efficacy through regulation of transport and metabolism. Seven of the ten users are currently supported by NIH grants, and we expect additional NIH support as the newly added faculty establish their UGA research programs. The requested instrumentation will allow them that access to NMR instrumentation needed for their current research, and it will allow them to adopt new experimental protocols that allow them to attack ever more complex problems in the future.

 描述（由申请人提供）：建议对主要用于结构生物学应用的现有600 MHz NMR光谱仪进行升级。此次升级将用新的Bruker Avance III HD 600高性能数字NMR控制台取代已有17年历史的Varian INOVA控制台，并使用Bruker冷冻平台取代现有的冷冻通道。升级后将允许使用高灵敏度冷冻探针，该探针针对13 C（和15 N）检测进行了优化，同时沿着1H和2H去耦。该仪器被放置在一个人员配备齐全，支持核磁共振设施，服务于格鲁吉亚（UGA）科学界的大学，以及整个地区的一些其他科学家。以高灵敏度直接观察13 C的能力将为该设施增加一种独特的能力，这种能力在生物分子系统的表征中越来越重要。这些系统包括大蛋白质或作为大复合物的一部分的蛋白质、具有固有无序区域的蛋白质和具有大量非质子化碳位点的天然产物（包括一些聚糖）。除了这种独特的能力，该系统将执行，与非低温系统的灵敏度相当或更高，传统的质子检测三重共振实验，已成为如此重要的生物医学重要的蛋白质的结构确定。在后一种情况下，更可靠和最新的系统将增加重要的能力，为越来越多的NMR用户提供服务，这些用户在UGA从事基于NMR的结构工作。七个主要用户和三个次要用户提供了研究说明，作为本提案的一部分。他们的项目影响的领域包括：聚糖在发育和转移中的作用，响应环境压力的蛋白质修饰，细胞信号传导和免疫反应中的蛋白质相互作用，毒力因子的调节 在病原菌中，并通过调节转运和代谢来提高药效。十个用户中有七个目前由NIH赠款，我们希望更多的NIH支持，因为新增的教师建立他们的UGA研究计划。所要求的仪器将使他们能够获得当前研究所需的NMR仪器，并使他们能够采用新的实验方案，使他们能够在未来解决更复杂的问题。

项目成果

Dissolved organic metabolite extraction from high‐salt media

从高盐介质中提取溶解的有机代谢物

• DOI: 10.1002/nbm.4797
• 发表时间: 2022
• 期刊: NMR in Biomedicine
• 影响因子: 2.9
• 作者: Holderman, Nicole R.;Ferrer‐González, Frank X.;Glushka, John;Moran, Mary Ann;Edison, Arthur S.
• 通讯作者: Edison, Arthur S.